Influence of pancreatic status on circulating plasma sterols in patients with cystic fibrosis

Monica Gelzo; Paola Iacotucci; Concetta Sica; Renato Liguori; Marika Comegna; Vincenzo Carnovale; Antonio Dello Russo; Gaetano Corso; Giuseppe Castaldo

doi:10.1515/cclm-2019-1112

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Article

Influence of pancreatic status on circulating plasma sterols in patients with cystic fibrosis

Monica Gelzo , Paola Iacotucci , Concetta Sica , Renato Liguori , Marika Comegna , Vincenzo Carnovale , Antonio Dello Russo , Gaetano Corso and Giuseppe Castaldo

Published/Copyright: February 14, 2020

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From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 58 Issue 10

Abstract

Background

Patients with cystic fibrosis (CF) have a reduced intestinal absorption of cholesterol and in a preliminary study we observed differences in plasma sterol profile between patients with pancreatic sufficiency (PS) and those with pancreatic insufficiency (PI). Therefore, we hypothesized that the sterol analysis may contribute to study the digestion and absorption state of lipids in patients with CF. To this aim we evaluated plasma sterols in a significant number of adult patients with CF in relation to the pancreatic status.

Methods

Beside cholesterol, we measured phytosterols and lathosterol as markers of intestinal absorption and hepatic biosynthesis, respectively, by gas-chromatography in plasma of adult CF patients with pancreatic sufficiency (PS-CF, n = 57), insufficiency (PI-CF, n = 97) and healthy subjects (control group, CT, n = 71).

Results

PI-CF patients had cholesterol and phytosterols levels significantly lower than PS-CF and CT (p < 5 × 10⁻¹⁰) suggesting a reduced intestinal absorption of sterols related to PI. Instead, lathosterol was significantly higher in PI-CF patients than PS-CF and CT (p < 0.0003) indicating an enhanced cholesterol biosynthesis. In PI-CF patients, phytosterols positively correlate with vitamin E (p = 0.004). Both the classes of molecules need cholesterol esterase for the intestinal digestion, thus the reduced levels of such lipids in serum from PI-CF patients may depend on a reduced enzyme activity, despite the pancreatic enzyme supplementation in all PI-CF patients.

Conclusions

A plasma sterols profile may be useful to evaluate the metabolic status of lipids in adult patients with CF and could help to manage the pancreatic enzyme supplementation therapy.

Keywords: cholesterol; cystic fibrosis; gas chromatography; pancreatic insufficiency

Funding source: Regione Campania

Award Identifier / Grant number: Quota vincolata per la prevenzione e cura della Fi

Funding statement: Funder Name: Regione Campania, Funder Id: http://dx.doi.org/10.13039/501100003852, Grant Number: Quota vincolata per la prevenzione e cura della Fi.

Acknowledgments

Grants from Ministero della Salute (Rome, Italy) L.548/93 and Campania Region (regional research funding quote of years 2013–15) are gratefully acknowledged.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2019-10-28

Accepted: 2019-12-26

Published Online: 2020-02-14

Published in Print: 2020-09-25

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Articles in the same Issue

https://doi.org/10.1515/cclm-2019-1112

Keywords for this article

cholesterol; cystic fibrosis; gas chromatography; pancreatic insufficiency