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Comparison of capillary electrophoresis and high performance liquid chromatography for detection and quantification of hemoglobin New York

  • Li You-Qiong , Huang Hui-Ping , Chen Zhi-Zhong , Zhao Lin , Liang Liang , Qin Gui-Fang and Mo Yun EMAIL logo
Published/Copyright: June 6, 2015
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 54 Issue 1

Abstract

Background: Hemoglobin (Hb) New York [β113 (G15) Val→Glu, GTG→GAG], also known as Hb Kaohsiung, is one of the most common Hb variants in South China. Currently, most used screening methods for hemoglobinopathies in South China are high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). However, there is no study comparing the performance of CE and HPLC in the detection of Hb New York.

Methods: In total 15 samples (including 13 adult blood samples and 2 cord blood samples) with heterozygous Hb New York were analyzed by CE and HPLC. Levels of Hb New York, HbA2, Hb, mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were compared within the two groups.

Results: All 15 cases (100%) were detected by CE, whereas none was detected by HPLC. Mean values of Hb New York and HbA2 in adult heterozygous group were (43.82±2.47)% and (2.85±0.31)%, respectively; mean values for cord blood group were (7.95±0.78)% and (0.30±0.14)%.

Conclusions: CE allows the detection of Hb New York, while this variant is not separated from Hb A on HPLC. CE may be the preferred method for hemoglobinopathy screening in areas with high prevalence of Hb New York.

Keywords: capillary electrophoresis (CE); hemoglobin New York; high performance liquid chromatography (HPLC)
Corresponding author: Prof. Mo Yun, Department of Obstetrics and Gynecology, People’s Hospital of Guangxi Zhuang Autonomous Region, 6 Taoyuan Road, Nanning 530021, P.R. China, Phone: +86 0771 2186127, E-mail:

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. Xiong F, Sun M, Zhang X, Cai R, Zhou Y, Lou J, et al. Molecular epidemiological survey of haemoglobinopathies in the Guangxi Zhuang Autonomous region of southern China. Clin Genet 2010;78:139–48.10.1111/j.1399-0004.2010.01430.xSearch in Google Scholar PubMed

2. Li YQ, Huang HP, Qin GF, Yang WH, Lao ZC. Phenotype and genotype analysis of hemoglobin E. Zhonghua Xue Ye Xue Za Zhi 2012;33:861–4.Search in Google Scholar

3. Li YQ, Huang HP, Yang WH, Chen ZZ, Zhao L, Huang HY, et al. Clinical and molecular characteristics of hemoglobin New York in Guangxi populations. Zhonghua Xue Ye Xue Za Zhi 2013;34:696–9.Search in Google Scholar

4. McFarlane A, Warkentin TE, Cartín W. A novel sickling hemoglobinopathy. N Engl J Med 2011;365:1548–9.10.1056/NEJMc1109418Search in Google Scholar PubMed

5. Calder D, Etienne-Julan M, Romana M, Watkins N, Knight-Madden JM. Sickle retinopathy in a person with hemoglobin s/New York disease. Case Rep Gent 2012;2012:136582.10.1155/2012/136582Search in Google Scholar PubMed PubMed Central

6. Keren DF, Hedstrom D, Gulbranson R, Ou CN, Bak R. Comparison of Sebia Capillarys capillary electrophoresis with the Primus high-pressure liquid chromatography in the evaluation of hemoglobinopathies. Am J Clin Pathol 2008;130:824–31.10.1309/AJCPQY80HZWHHGZFSearch in Google Scholar PubMed

7. Higgins T, Mack M, Khajuria A. Comparison of two methods for the quantification and identification of hemoglobin variants. Clin Biochem 2009;42:701–5.10.1016/j.clinbiochem.2009.01.004Search in Google Scholar PubMed

8. Winichagoon P, Svasti S, Munkongdee T, Chaiya W, Boonmongkol P, Chantrakul N, et al. Rapid diagnosis of thalassemias and other hemoglobinopathies by capillary electrophoresis system. Transl Res 2008;152:178–84.10.1016/j.trsl.2008.08.004Search in Google Scholar PubMed

9. Li DZ. Hemoglobin New York is not a matter in prenatal screening and diagnosis for beta-thalassemia. Pediatr Hematol Oncol 2008;25:769–71.10.1080/08880010802435435Search in Google Scholar PubMed

10. Joutovsky A, Hadzi-Nesic J, Nardi MA. HPLC retention time as a diagnostic tool for hemoglobin variants and hemoglobinopathies: a study of 60000 samples in a clinical diagnostic laboratory. Clin Chem 2004;50:1736–47.10.1373/clinchem.2004.034991Search in Google Scholar PubMed

11. Uaprasert N, Rojnuckarin P, Settapiboon R, Amornsiriwat S, Sutcharitchan P. Clinical and hematological characteristics of uncommon beta-globin variants in Thailand. Int J Hematol 2009;89:568–71.10.1007/s12185-009-0334-1Search in Google Scholar PubMed

12. Li DZ, Zhou JY, Xie XM, Liao C. Association of Hb New York with Hb E and alpha(0)-thalassemia in a Chinese woman identified by Sebia CapillaryS2 system. Hemoglobin 2012;36:157–60.10.3109/03630269.2012.658129Search in Google Scholar PubMed

Received: 2015-3-8
Accepted: 2015-4-27
Published Online: 2015-6-6
Published in Print: 2016-1-1

©2016 by De Gruyter

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https://doi.org/10.1515/cclm-2015-0238
Keywords for this article
capillary electrophoresis (CE); hemoglobin New York; high performance liquid chromatography (HPLC)

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Building a bridge to safe diagnosis in health care. The role of the clinical laboratory
  4. Capillary electrophoresis for the screening and diagnosis of inherited hemoglobin disorders. Ready for prime time?
  5. Reviews
  6. Hyperuricemia as risk factor for coronary heart disease incidence and mortality in the general population: a systematic review and meta-analysis
  7. Towards biomarker-based tests that can facilitate decisions about prevention and management of preeclampsia in low-resource settings
  8. Mini Review
  9. Clinical relevance of sST2 in cardiac diseases
  10. General Clinical Chemistry and Laboratory Medicine
  11. Preservation of urine free catecholamines and their free O-methylated metabolites with citric acid as an alternative to hydrochloric acid for LC-MS/MS-based analyses
  12. Performance characteristics of the VIDAS® 25-OH Vitamin D Total assay – comparison with four immunoassays and two liquid chromatography-tandem mass spectrometry methods in a multicentric study
  13. Differentiation of acute pyelonephritis from other febrile states in children using urinary neutrophil gelatinase-associated lipocalin (uNGAL)
  14. Early postoperative C-terminal agrin fragment (CAF) serum levels predict graft loss and proteinuria in renal transplant recipients
  15. Homocitrulline: a new marker for differentiating acute from chronic renal failure
  16. Hematology and Coagulation
  17. Development of hemoglobin typing control materials for laboratory investigation of thalassemia and hemoglobinopathies
  18. Comparison of capillary electrophoresis and high performance liquid chromatography for detection and quantification of hemoglobin New York
  19. Prevalence of hemostatic alterations in patients with recurrent spontaneous subconjunctival hemorrhage
  20. Reference Values and Biological Variations
  21. Reference intervals for 24 laboratory parameters determined in 24-hour urine collections
  22. First trimester PAPP-A2, PAPP-A and hCGβ in small-for-gestational-age pregnancies
  23. Cancer Diagnostics
  24. Clinical utility of one versus two faecal immunochemical test samples in the detection of advanced colorectal neoplasia in symptomatic patients
  25. Cardiovascular Diseases
  26. Time from symptom onset influences high-sensitivity troponin T diagnostic accuracy for the diagnosis of acute myocardial infarction
  27. Prognostic significance of serum albumin level changes in acute ischemic stroke: the role of biological and analytical variation
  28. Correlates of serum hepcidin levels and its association with cardiovascular disease in an elderly general population
  29. Infectious Diseases
  30. Usefulness of several biomarkers in the management of septic patients: C-reactive protein, procalcitonin, presepsin and mid-regional pro-adrenomedullin
  31. Diabetes
  32. Performance of strip-based glucose meters and cassette-based blood gas analyzer for monitoring glucose levels in a surgical intensive care setting
  33. Accuracy and precision assessment of a new blood glucose monitoring system
  34. Acknowledgment
  35. Acknowledgment
  36. Letter to the Editors
  37. Glypican-1 as a highly sensitive and specific pancreatic cancer biomarker
  38. Platelet microRNAs are not modulated by systemic heparin in acute coronary syndromes
  39. Concomitant appearance of two false positive peaks with a true monoclonal one in a patient with plasma cell myeloma
  40. Anti-streptavidin interferences in Roche thyroid immunoassays: a case report
  41. Evaluation of the new Methotrexate CMIA assay on the Architect i2000SR
  42. Liposomal interference on Sysmex XN-series body fluid mode
  43. Development of an immunomagnetic beads-based test and its application in influenza surveillance
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