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Optimizing centrifugation of coagulation samples in laboratory automation

  • Juliane Suchsland , Nele Friedrich , Anne Grotevendt , Anders Kallner , Jan Lüdemann , Matthias Nauck and Astrid Petersmann EMAIL logo
Published/Copyright: April 2, 2014
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Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 52 Issue 8

Abstract

Background: High acceleration centrifugation conditions are used in laboratory automation systems to reduce the turnaround time (TAT) of clinical chemistry samples, but not of coagulation samples. This often requires separate sample flows. The CLSI guideline and manufacturers recommendations for coagulation assays aim at reducing platelet counts. For measurement of prothrombin time (PT) and activated partial thromboplastin time (APTT) platelet counts (Plt) below 200×109/L are recommended. Other coagulation assays may require even lower platelet counts, e.g., less than 10×109/L. Unifying centrifugation conditions can facilitate the integration of coagulation samples in the overall workflow of a laboratory automation system.

Methods: We evaluated centrifugation conditions of coagulation samples by using high acceleration centrifugation conditions (5 min; 3280×g) in a single and two consecutive runs. Results of coagulation assays [PT, APTT, coagulation factor VIII (F. VIII) and protein S] and platelet counts were compared after the first and second centrifugation.

Results: Platelet counts below 200×109/L were obtained in all samples after the first centrifugation and less than 10×109/L was obtained in 73% of the samples after a second centrifugation. Passing-Bablok regression analyses showed an equal performance of PT, APTT and F. VIII after first and second centrifugation whereas protein S measurements require a second centrifugation.

Conclusions: Coagulation samples can be integrated into the workflow of a laboratory automation system using high acceleration centrifugation. A single centrifugation was sufficient for PT, APTT and F. VIII whereas two successive centrifugations appear to be sufficient for protein S activity.

Keywords: centrifugation; coagulation; laboratory automation system; pre-analytic phase; robotics; turnaround time; work flow
Corresponding author: Dr. med. Dipl. Biol. Astrid Petersmann, Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Ferdinand-Sauerbruch-Str., 17475 Greifswald, Germany, Phone: +49 3834 865670, E-mail:

Acknowledgments

We wish to thank BD Diagnostics for sponsoring parts of the reagents used for this study. Besides this there is no other conflict of interest.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research sponsoring played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

References

1. Mensel B, Wenzel U, Roser M, Ludemann J, Nauck M. Considerably reduced centrifugation time without increased hemolysis: evaluation of the new BD Vacutainer SSTTMII Advance. Clin Chem 2007;53:794–5.10.1373/clinchem.2006.079582Search in Google Scholar PubMed

2. Adcock DM. Collection, transport, and processing of blood specimens for testing plasma-based coagulation assays and molecular hemostasis assays: approved guideline, 5th ed. Wayne, PA: Clinical and Laboratory Standards Institute, 2008.Search in Google Scholar

3. Nelson S, Pritt A, Marlar RA. Rapid preparation of plasma for ‘Stat’ coagulation testing. Arch Pathol Lab Med 1994;118:175–6.Search in Google Scholar

4. Sedille-Mostafaie N, Engler H, Lutz S, Korte W. Advancing haemostasis automation – successful implementation of robotic centrifugation and sample processing in a tertiary service hospital. Clin Chem Lab Med 2013;51:1273–8.10.1515/cclm-2012-0625Search in Google Scholar PubMed

5. Streichert T, Otto B, Schnabel C, Nordholt G, Haddad M, Maric M, et al. Determination of hemolysis thresholds by the use of data loggers in pneumatic tube systems. Clin Chem 2011;57:1390–7.10.1373/clinchem.2011.167932Search in Google Scholar PubMed

6. Bundesärztekammer BÄ. Richtlinie der Bundesärztekammer zur Qualitätssicherung laboratoriumsmedizinischer Untersuchungen: BÄK Bundesärztekammer; 2013 Available from: http://www.bundesaerztekammer.de/page.asp?his=1.120.121.1047.6009. Accessed 11 September, 2013.Search in Google Scholar

7. Brien WF, Schaus MR, Cooper KE, O’Keefe BT, Inwood M. Lupus anticoagulant testing: effect of the platelet count on the activated partial thromboplastin time. Br J Biomed Sci 1993;50:114–6.Search in Google Scholar

8. Tripodi A, Valsecchi C, Chantarangkul V, Battaglioli T, Mannucci PM. Standardization of activated protein C resistance testing: effect of residual platelets in frozen plasmas assessed by commercial and home-made methods. Br J Haematol 2003;120:825–8.10.1046/j.1365-2141.2003.04171.xSearch in Google Scholar PubMed

9. Lippi G, Salvagno GL, Montagnana M, Manzato F, Guidi GC. Influence of the centrifuge time of primary plasma tubes on routine coagulation testing. Blood Coagul Fibrinolysis 2007;18:525–8.10.1097/MBC.0b013e3281eec945Search in Google Scholar PubMed

10. Boudaoud L, Divaret G, Marie P, Bezeaud A. Rapid centrifugation for routine coagulation testing. Ann Biol Clin (Paris) 2006;64:315–7.Search in Google Scholar

11. Sultan A. Five-minute preparation of platelet-poor plasma for routine coagulation testing. East Mediterr Health J 2010;16: 233–6.10.26719/2010.16.2.233Search in Google Scholar

Received: 2014-1-10
Accepted: 2014-3-5
Published Online: 2014-4-2
Published in Print: 2014-8-1

©2014 by Walter de Gruyter Berlin/Boston

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  7. Genetics and Molecular Diagnostics
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https://doi.org/10.1515/cclm-2014-0038
Keywords for this article
centrifugation; coagulation; laboratory automation system; pre-analytic phase; robotics; turnaround time; work flow

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. Colorectal cancer and screening programs: not only analytical issues
  4. Reviews
  5. Laboratory diagnostics of inherited platelet disorders
  6. Reticulated platelets: analytical aspects and clinical utility
  7. Genetics and Molecular Diagnostics
  8. Advanced tools for BRCA1/2 mutational screening: comparison between two methods for large genomic rearrangements (LGRs) detection
  9. General Clinical Chemistry and Laboratory Medicine
  10. Establishing, harmonizing and analyzing critical values in a large academic health center
  11. Standardization of DiaSorin and Roche automated third generation PTH assays with an International Standard: impact on clinical populations
  12. First fully automated immunoassay for anti-Müllerian hormone
  13. A multicenter evaluation of dysthyroxinemia in a defined patient cohort
  14. New biomarkers in diagnosis of early onset preeclampsia and imminent delivery prognosis
  15. Soluble Fms-like tyrosine kinase-1 to placental growth factor ratio in mid-pregnancy as a predictor of preterm preeclampsia in asymptomatic pregnant women
  16. Development of a new immunoassay for the detection of ethyl glucuronide (EtG) in meconium: validation with authentic specimens analyzed using LC-MS/MS. Preliminary results
  17. Optimizing centrifugation of coagulation samples in laboratory automation
  18. Evaluation of the automated coagulation analyzer CS-5100 and its utility in high throughput laboratories
  19. A new sampling device for faecal immunochemical testing: haemoglobin stability is still an open issue
  20. Reference Values
  21. Faecal haemoglobin concentrations vary with sex and age, but data are not transferable across geography for colorectal cancer screening
  22. Cancer Diagnostics
  23. Enhanced miR-182 transcription is a predictor of poor overall survival in colorectal adenocarcinoma patients
  24. Importance of promoter methylation of GATA4 and TP53 genes in endometrioid carcinoma of endometrium
  25. Frequent methylation of HOXA9 gene in tumor tissues and plasma samples from human hepatocellular carcinomas
  26. Letters to the Editor
  27. Further comments on “Critical review of laboratory investigations in clinical practice guidelines: proposals for the description of investigation”
  28. A questionnaire study among nurses: awareness of blood and urine sample collection procedures
  29. Measurement uncertainty and clinical interpretation of measurement results
  30. Laboratory automation: how will you select the boarding assays?
  31. Improvement and evaluation of a 1,2-dioleoylglycerol method for measuring pancreatic lipase catalytic activity in serum
  32. The novel variant p.Ser465Leu in the PCSK9 gene does not account for the decreased LDLR activity in members of a FH family
  33. 1,5 Anhydroglucitol serum concentration as a biomarker for screening gestational diabetes in early pregnancy
  34. A rare condition: IgE type monoclonal gammopathy of undetermined significance
  35. Laboratory analysis of intraosseous blood: bad to the bone?
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