Platelet oxidative stress and systemic inflammation in chronic spontaneous urticaria

Medha Rajappa; Laxmisha Chandrashekar; Indhumathi Sundar; Malathi Munisamy; P. H. Ananthanarayanan; Devinder Mohan Thappa; Pampa Ch Toi

doi:10.1515/cclm-2012-0897

Article

Platelet oxidative stress and systemic inflammation in chronic spontaneous urticaria

Medha Rajappa , Laxmisha Chandrashekar , Indhumathi Sundar , Malathi Munisamy , P. H. Ananthanarayanan , Devinder Mohan Thappa and Pampa Ch Toi

Published/Copyright: April 9, 2013

Published by

Become an author with De Gruyter Brill

Submit Manuscript Author Information

From the journal Clinical Chemistry and Laboratory Medicine Volume 51 Issue 9

Abstract

Background: Recent studies implicate the role of immune-inflammatory responses in chronic spontaneous urticaria (CSU). Although it is well known that platelets release inflammatory mediators and reactive oxygen species upon activation, their role in CSU is poorly characterized. The present study was designed to evaluate platelet oxidative stress [platelet malondialdehyde (MDA), platelet superoxide dismutase (SOD), platelet glutathione peroxidase (GPx)] and systemic inflammatory markers [plasma Interleukin-6 (IL-6), high sensitivity C-reactive protein (hs-CRP)] in patients with CSU and their association with disease severity.

Methods: Forty-five patients with CSU and 45 age- and gender-matched healthy controls were included in the study. Severity grading was completed according to the urticaria severity score (USS). Autologous plasma skin test (APST) was done in all patients with CSU. Platelet MDA, SOD and GPx and inflammatory markers plasma IL-6 and hs-CRP were assayed in all study participants.

Results: In patients with CSU, platelet SOD and GPx were significantly lowered, while platelet MDA levels were significantly elevated in comparison to healthy controls. Both IL-6 and hs-CRP were significantly elevated in patients with CSU and correlated with platelet oxidative stress parameters (p<0.05). Platelet MDA, SOD and GPx and inflammatory markers (plasma IL-6 and hs-CRP) showed a significant correlation with USS in patients with CSU.

Conclusions: Our results indicate significant systemic inflammation and platelet oxidative stress in patients with CSU.

Keywords: chronic spontaneous urticaria; glutathione peroxidase; high sensitivity C- reactive protein; interleukin-6; malondialdehyde; platelet oxidative stress; superoxide dismutase

Corresponding author: Dr. Laxmisha Chandrashekar, Assistant Professor, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Department of Dermatology, Puducherry 605006, India, Phone: +91 9894119058, Fax: +91 413 2272067

The present study was funded by the JIPMER Intramural Research Grant. Funding from JIPMER, Puducherry, India is gratefully acknowledged.

Conflict of interest statement

Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

Research funding: The present study was funded by the JIPMER Intramural Research Grant. Funding from JIPMER, Puducherry, India is gratefully acknowledged.

Employment or leadership: None declared.

Honorarium: None declared.

References

1. Kasperska-Zajac A, Brzoza Z, Polaniak R, Rogala B, Birkner E. Markers of antioxidant defence system and lipid peroxidation in peripheral blood of female patients with chronic idiopathic urticaria. Arch Dermatol Res 2007;298:499–503.10.1007/s00403-006-0724-7Search in Google Scholar

2. Briganti S, Cristaudo A, D’Argento V, Cassano N, Turbino L, Guarrera M, et al. Oxidative stress in physical urticarias. Clin Exp Dermatol 2001;26:284–8.10.1046/j.1365-2230.2001.00817.xSearch in Google Scholar

3. Raho G, Cassano N, D’Argento V, Vena GA, Zanotti F. Over-expression of Mn-superoxide dismutase as a marker of oxidative stress in lesional skin of chronic idiopathic urticaria. Clin Exp Dermatol 2003;28:318–20.10.1046/j.1365-2230.2003.01264.xSearch in Google Scholar

4. Cassano N, Raho G, Filieri M, D’Argento V, Amoruso A, Filotico R, et al. Influence of desloratadine on oxidative stress markers in patients with chronic idiopathic urticaria. Int J Dermatol 2006;45:394–6.10.1111/j.1365-4632.2006.02467.xSearch in Google Scholar

5. Sagdic A, Sener O, Bulucu F, Karadurmus N, Yamanel L, Tasci C, et al. Oxidative stress status in patients with chronic idiopathic urticaria. Allergol Immunopathol (Madr) 2011;39:150–3.10.1016/j.aller.2010.06.012Search in Google Scholar

6. Xiaoming L, Wanxiang S, Khalaf AT, Jiquan S, Jinquan T. Relation between autoimmunity chronic urticaria and the levels of plasma prothrombin F1+2. Am J App Sci 2007;4:965–9.10.3844/ajassp.2007.965.969Search in Google Scholar

7. Kasperska-Zajac A. Acute-phase response in chronic urticaria.J Eur Acad Dermatol Venereol 2012;26:665–72.10.1111/j.1468-3083.2011.04366.xSearch in Google Scholar

8. Zuberbier T, Bindslev-Jensen C, Canonica W, Grattan CE, Greaves MW, Henz BM, et al. EAACI/GA2LEN/EDF guideline: definition, classification and diagnosis of urticaria. Allergy 2006;61:316–20.10.1111/j.1398-9995.2005.00964.xSearch in Google Scholar

9. Asero R, Tedeschi A, Riboldi P, Cugno M. Plasma of patients with chronic urticaria shows signs of thrombin generation, and its intradermal injection causes wheal-and-flare reactions much more frequently than autologous serum. J Allergy Clin Immunol 2006;117:1113–7.10.1016/j.jaci.2005.12.1343Search in Google Scholar

10. Erel O, Vural H, Aksoy N, Aslan G, Ulukanligil M. Oxidative stress of platelets and thrombocytopenia in patients with vivax malaria. Clin Biochem 2001;34:341–4.10.1016/S0009-9120(01)00221-1Search in Google Scholar

11. Agarwal R, Chase SD. Rapid, fluorimetric-liquid chromatographic determination of malondialdehyde in biological samples. J Chromatogr B Analyt Technol Biomed Life Sci 2002;75:121–6.10.1016/S1570-0232(02)00273-8Search in Google Scholar

12. Lowry OH, Rosebrough NL, Farr AL, Randall RF. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75.10.1016/S0021-9258(19)52451-6Search in Google Scholar

13. Dos Santos JC, Azor MH, Nojima VY, Lourenço FD, Prearo E, Maruta CW, et al. Increased circulating pro-inflammatory cytokines and imbalanced regulatory T-cell cytokines production in chronic idiopathic urticaria. Int Immunopharmacol 2008;8:1433–40.10.1016/j.intimp.2008.05.016Search in Google Scholar PubMed

14. Magen E, Mishal J, Zeldin Y, Feldman V, Kidon M, Schlesinger M, et al. Increased mean platelet volume and C-reactive protein levels in patients with chronic urticaria with a positive autologous serum skin test. Am J Med Sci 2010;339:504–810.1097/MAJ.0b013e3181db6ed5Search in Google Scholar PubMed

15. Kasperska-Zajac A, Brzoza Z, Rogala B. Plasma concentration of interleukin-6 (IL-6), and its relationship with circulating concentration of dehydroepiandrosterone sulfate (DHEA-S) in patients with chronic idiopathic urticaria. Cytokine 2007;39:142–6.10.1016/j.cyto.2007.06.008Search in Google Scholar PubMed

16. Kasperska-Zajac A, Sztylc J, Machura E, Jop G. Plasma IL-6 concentration correlates with clinical disease activity and serum C-reactive protein concentration in chronic urticaria patients. Clin Exp Allergy 2011;41:1386–91.10.1111/j.1365-2222.2011.03789.xSearch in Google Scholar PubMed

17. Pashkow FJ. Oxidative stress and inflammation in heart disease: do antioxidants have a role in treatment and/or prevention? Int J Inflam 2011;2011:514623.10.4061/2011/514623Search in Google Scholar PubMed PubMed Central

18. Kasperska-Zajac A, Rogala B, Nowakowski M. Assessment of platelet activity as expressed by plasma levels of platelet factor 4 and beta-thromboglobulin in patients with chronic idiopathic urticaria. Exp Dermatol 2005;14:515–8.10.1111/j.0906-6705.2005.00311.xSearch in Google Scholar PubMed

Received: 2012-12-22

Accepted: 2013-3-18

Published Online: 2013-04-09

Published in Print: 2013-09-01

You are currently not able to access this content.

Articles in the same Issue

https://doi.org/10.1515/cclm-2012-0897

Keywords for this article

chronic spontaneous urticaria; glutathione peroxidase; high sensitivity C- reactive protein; interleukin-6; malondialdehyde; platelet oxidative stress; superoxide dismutase