Cloning differentially expressed salt induced cDNAs from Dunaliella salina under super saturated salt stress using subtractive hybridization
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Avinash Mishra
Abstract
Differentially expressed genes in Dunaliella salina grown under super saturated salt stress (5.5 m) were isolated by subtractive hybridization, cloned and classified for further biotechnological and industrial exploration. Sequence homology analysis showed that 26% of the library represented salt responsive genes for metabolic enzymes, viz., fructose-1,6-diphosphate aldolase (AldP), glucose-6-phosphate dehydrogenase, carbonic anhydrase, phosphoglycerate kinase etc., while 6% of library clones contained hypothetical/unknown genes. Six clones (8% of the library) contained genes that differentially expressed under salt stress, however, 6–8% of the library represented genes over expressed under salt stress and involved in photosynthesis, defense, cell rescue (chaperon/protease inhibitor) and protein sorting. About 39% were other genes of which 6% and 14% were transcription factors and house keeping genes (putative enzymes/signaling) respectively, while the remaining 19% of genes had miscellaneous functions.
©2011 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Research Articles
- Succession patterns in algal turf vegetation on a Caribbean coral reef
- Carpospore and tetraspore release and survival in Chondracanthus squarrulosus (Rhodophyta: Gigartinaceae) from the Gulf of California
- Morphology and taxonomy of Anadyomene species (Cladophorales, Chlorophyta) from Bahia, Brazil
- Sargassum polyceratium (Phaeophyceae, Fucaceae) surface molecule activity towards fouling organisms and embryonic development of benthic species
- Colpomenia claytonii sp. nov. (Scytosiphonaceae, Phaeophyceae) based on morphology and mitochondrial cox3 sequences
- A survey of the benthic aquatic flora in transitional water systems of Greece and Cyprus (Mediterranean Sea)
- Morphological study of the genus Cocconeis Ehrenberg (Bacillariophyceae) collected during the 1897–1899 Belgian Antarctic Expedition
- Cloning differentially expressed salt induced cDNAs from Dunaliella salina under super saturated salt stress using subtractive hybridization
- Antioxidant response of the microalga Dunaliella salina under salt stress
- Temporal variation in growth and reproduction of Enhalus acoroides (L.f.) Royle in a monospecific meadow in Haad Chao Mai National Park, Trang Province, Thailand
- Lignicolous marine fungi from Yokosuka, Japan
