Antimicrobial activities of marine fungi from Malaysia
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Nazura Zainuddin
Abstract
In a systematic screening effort, extracts of marine fungi from Malaysia were investigated for antimicrobial activity and potentially active secondary metabolites. In preliminary experiments, the plug assay method was employed to screen 152 strains for antimicrobial activity. Of these, 82 exhibited biological activity, with most of the active strains (90.2%) having antibacterial activity, mainly towards Gram-positive bacteria, while only 9.6% had antifungal activity. On the basis of these results, five marine fungi, Fasciatispora nypae, Caryosporella rhizophorae, Melaspilea mangrovei, Leptosphaeria sp. and ascomycete strain 19 (NF) were selected for further investigation to confirm their biological activity by the disc diffusion assay method. The selected species had various degrees of activity against the test microorganisms, depending on culture conditions (stationary vs. shaking cultures) and incubation time (10–25 days). Only F. nypae showed a wider range of antifungal and antibacterial activity as compared to the remaining fungal strains under investigation. Therefore, bioactivity-guided fractionation was undertaken to isolate the active principles, resulting in the characterisation of 2,2,7-trimethyl-2H-chromen-5-ol (1) which had antimicrobial activity towards test microorganisms. The structure of 1, which previously had only been reported as a synthetic intermediate, but not as a natural product, was elucidated by mass spectrometry in conjunction with one- and two-dimensional NMR spectroscopy.
©2010 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Guest editorial
- 11th International Marine and Freshwater Mycology Symposium, Taichung, Taiwan R.O.C., November 2009
- Review
- A review on deep-sea fungi: occurrence, diversity and adaptations
- Research articles
- Sedecimiella taiwanensis gen. et sp. nov., a marine mangrove fungus in the Hypocreales (Hypocreomycetidae, Ascomycota)
- Dibenzofurans from the marine sponge-derived ascomycete Super1F1-09
- Antimicrobial activities of marine fungi from Malaysia
- Diversity and abundance of lignicolous marine fungi from the east and west coasts of Peninsular Malaysia and Sabah (Borneo Island)
- Fungal colonization and breakdown of sedge (Cyperus malaccensis Lam.) in an Indian mangrove
- Occurrence and distribution of fungi in a mangrove forest on Siargao Island, Philippines
- Biodiversity of marine fungi in Malaysian mangroves
- Endophytic fungi from mangrove plant species of Thailand: their antimicrobial and anticancer potentials
- Fungal communities in bunker C oil-impacted sites off southern Guimaras, Philippines: a post-spill assessment of Solar 1 oil spill
- Potential use of marine arenicolous ascomycetes as bioindicators of ecosystem disturbance on sandy Cancun beaches: Corollospora maritima as a candidate species
- Effects of Cu(II) and Zn(II) on growth and cell morphology of thraustochytrids isolated from fallen mangrove leaves in Taiwan
- Marine-derived fungi from Kappaphycus alvarezii and K. striatum as potential causative agents of ice-ice disease in farmed seaweeds
- Short communication
- Fungal diversity in bottom sediments of the Kara Sea
- Reviewer acknowledgement Bot. Mar. volume 53 (2010)
Articles in the same Issue
- Guest editorial
- 11th International Marine and Freshwater Mycology Symposium, Taichung, Taiwan R.O.C., November 2009
- Review
- A review on deep-sea fungi: occurrence, diversity and adaptations
- Research articles
- Sedecimiella taiwanensis gen. et sp. nov., a marine mangrove fungus in the Hypocreales (Hypocreomycetidae, Ascomycota)
- Dibenzofurans from the marine sponge-derived ascomycete Super1F1-09
- Antimicrobial activities of marine fungi from Malaysia
- Diversity and abundance of lignicolous marine fungi from the east and west coasts of Peninsular Malaysia and Sabah (Borneo Island)
- Fungal colonization and breakdown of sedge (Cyperus malaccensis Lam.) in an Indian mangrove
- Occurrence and distribution of fungi in a mangrove forest on Siargao Island, Philippines
- Biodiversity of marine fungi in Malaysian mangroves
- Endophytic fungi from mangrove plant species of Thailand: their antimicrobial and anticancer potentials
- Fungal communities in bunker C oil-impacted sites off southern Guimaras, Philippines: a post-spill assessment of Solar 1 oil spill
- Potential use of marine arenicolous ascomycetes as bioindicators of ecosystem disturbance on sandy Cancun beaches: Corollospora maritima as a candidate species
- Effects of Cu(II) and Zn(II) on growth and cell morphology of thraustochytrids isolated from fallen mangrove leaves in Taiwan
- Marine-derived fungi from Kappaphycus alvarezii and K. striatum as potential causative agents of ice-ice disease in farmed seaweeds
- Short communication
- Fungal diversity in bottom sediments of the Kara Sea
- Reviewer acknowledgement Bot. Mar. volume 53 (2010)
