Proposal of Cryptonemia floridana comb. nov. (Halymeniaceae, Rhodophyta) based on rbcL gene analysis of collections from type locality and female reproductive morphology
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Luanda Pereira Soares
Luanda Pereira Soares is Adjunct Professor at the Department of Systematics and Ecology (DSE), Universidade Federal da Paraíba (UFPB), Brazil. She was awarded a PhD degree in Plant Biodiversity from the Institute of Environmental Research (IPA, formerly Institute of Botany), São Paulo, Brazil. Her main expertise is taxonomy and molecular phylogeny of marine macroalgae, especially red algae. Her current research includes reassessment of type specimens, endemic and rare marine algae occurring in Brazil and American Atlantic.
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Silvia M.P.B. Guimarães
Silvia M.P.B. Guimarães is Associate Researcher at the Institute of Environmental Research (IPA, formerly Institute of Botany), São Paulo, Brazil. She received her Master’s degree and her PhD degree in Botany from the University of São Paulo for her work on the taxonomy of marine algae, mainly Rhodophyta. Presently, she works in the following areas: taxonomy of marine algae, algae of the infralittoral region and algae associated with rhodoliths beds.
Thomas Sauvage is a freelance researcher and entrepreneur in the field of Genomics. He received his PhD degree in Environmental and Evolutionary Biology at the University of Louisiana at Lafayette for his studies on cryptic diversity of siphonous green algae with next generation sequencing. His research interests lie in the molecular diversity of marine algae, biogeography and emerging sequencing technologies such as Nanopore sequencing.
Larissa A.H. dos Santos is a freelance researcher. She received her Master degree in Oceans and Earth Dynamics at the Fluminense Federal University in Rio de Janeiro, Brazil. Her research interests bear on the chemical and molecular diversity of marine organisms.
Mutue T. Fujii is Scientific Researcher at the Environmental Research Institute (IPA, formerly Institute of Botany), São Paulo, Brazil and Director of the Knowledge Management Department, at the same institution. She received her Master and PhD degrees in Plant Biology from the São Paulo State University for her studies on morphological, chemical and cytogenetical approaches on
Laurencia s.l. (Rhodophyta) from Brazil. Her areas of interest are biodiversity of marine algae, with emphasis on taxonomy, molecular phylogeny, phylogeography and biogeography.
Abstract
The taxonomic position of Halymenia floridana is a long-standing issue that needed to be assessed using DNA sequences of material from the type locality (Florida, USA). Here, we examined the vegetative and reproductive morpho-anatomy, and rbcL sequence data of topotypes and additional specimens from Brazil. Our results resolved the topotypes of ‘H. floridana’ within the genus Cryptonemia, in a clade harboring two closely related sister species, C. bermudensis and C. lacunicola. The rbcL haplotype of ‘H. floridana’ from Florida was also identical to previously and newly sequenced specimens from Brazil. Therefore, the new combination Cryptonemia floridana comb. nov. is herein established. Cryptonemia floridana exhibits entire to lobed foliose blades up to 12 cm high, a short basal midrib, medulla with conspicuous stellate ganglia and mostly periclinal filaments. The auxiliary cell is the first or the second cell of a third-order ampullar filament, and secondary connecting filaments are rare and cut off from the fusion cell. Our study is the first to provide the pre- and postfertilization stages in a Cryptonemia species, with generic status confirmed by DNA sequences. Numerous tropical Cryptonemia species await molecular confirmation and assessment of reproductive structures towards a better circumscription of the genus and its species diversity.
Funding source: Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
Award Identifier / Grant number: 2016/50370-7
Award Identifier / Grant number: 2017/50341-0
Funding source: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Award Identifier / Grant number: Bolsista CAPES/BRASIL 88887.515974/2020-00
Funding source: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Award Identifier / Grant number: Productivity Fellowship (311195/2021-0)
Funding source: G.E. Burch postdoctoral fellowship award
About the authors
Luanda Pereira Soares is Adjunct Professor at the Department of Systematics and Ecology (DSE), Universidade Federal da Paraíba (UFPB), Brazil. She was awarded a PhD degree in Plant Biodiversity from the Institute of Environmental Research (IPA, formerly Institute of Botany), São Paulo, Brazil. Her main expertise is taxonomy and molecular phylogeny of marine macroalgae, especially red algae. Her current research includes reassessment of type specimens, endemic and rare marine algae occurring in Brazil and American Atlantic.
Silvia M.P.B. Guimarães is Associate Researcher at the Institute of Environmental Research (IPA, formerly Institute of Botany), São Paulo, Brazil. She received her Master’s degree and her PhD degree in Botany from the University of São Paulo for her work on the taxonomy of marine algae, mainly Rhodophyta. Presently, she works in the following areas: taxonomy of marine algae, algae of the infralittoral region and algae associated with rhodoliths beds.
Thomas Sauvage is a freelance researcher and entrepreneur in the field of Genomics. He received his PhD degree in Environmental and Evolutionary Biology at the University of Louisiana at Lafayette for his studies on cryptic diversity of siphonous green algae with next generation sequencing. His research interests lie in the molecular diversity of marine algae, biogeography and emerging sequencing technologies such as Nanopore sequencing.
Larissa A.H. dos Santos is a freelance researcher. She received her Master degree in Oceans and Earth Dynamics at the Fluminense Federal University in Rio de Janeiro, Brazil. Her research interests bear on the chemical and molecular diversity of marine organisms.
Mutue T. Fujii is Scientific Researcher at the Environmental Research Institute (IPA, formerly Institute of Botany), São Paulo, Brazil and Director of the Knowledge Management Department, at the same institution. She received her Master and PhD degrees in Plant Biology from the São Paulo State University for her studies on morphological, chemical and cytogenetical approaches on Laurencia s.l. (Rhodophyta) from Brazil. Her areas of interest are biodiversity of marine algae, with emphasis on taxonomy, molecular phylogeny, phylogeography and biogeography.
Acknowledgements
We thank Patrik Frödén, Assistant Curator of the LD Herbarium, Lund University, for providing images of Agardh’s specimens of Halymenia floridana.
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Research ethics: This article is original and has not been submitted elsewhere. It does not violate any copyright or other personal proprietary right of any person or entity.
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Author contributions: Luanda P. Soares: investigation, sampling, morphological and molecular data acquisition, writing – original draft. Silvia M.P.B. Guimarães: sampling, morphological data acquisition, writing – review. Thomas Sauvage: sampling, molecular data acquisition, writing – review. Larrisa A.H. dos Santos: sampling, molecular data acquisition. Helena R. Fragoso: molecular data acquisition. Mutue T. Fujii: conceptualization, supervision, sampling, funding acquisition, data analysis, writing – review. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors declare no competing interests regarding this article.
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Research funding: LPS thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Bolsista CAPES/BRASIL 88887.515974/2020-00) and MTF thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the Productivity Fellowship (311195/2021-0). TS acknowledges support from a G.E. Burch postdoctoral fellowship award that allowed residency and research in Florida at SMS from 2016 to 2019. This study was partially supported by research grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2016/50370-7, and 2017/50341-0).
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Data availability: The raw data can be obtained on request from the corresponding author.
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Supplementary Material
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Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and Ecology
- Gambierdiscus carpenteri (Dinophyceae) from Bahía de La Paz, Gulf of California: morphology, genetic affinities, and mouse toxicity
- New record of the green macroalga Gayralia brasiliensis (Ulotrichales, Chlorophyta) in Singapore
- Taxonomy/Phylogeny and Biogeography
- Three new Lobophora species (Dictyotales, Phaeophyceae) from Phuket on the west coast of Thailand
- Proposal of Cryptonemia floridana comb. nov. (Halymeniaceae, Rhodophyta) based on rbcL gene analysis of collections from type locality and female reproductive morphology
- Kapraunia silviae (Rhodomelaceae, Rhodophyta), a new species from the South Atlantic Ocean
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- Morphology and molecular phylogeny of Gonyaulax kunsanensis sp. nov. (Gonyaulacales, Dinophyceae) from Korean coastal waters
- Introducing a new species, Vaginatispora acrostichi (Lophiostomataceae), based on morphology and multigene phylogeny
- Genomics
- Evaluation of cryptic invasion in Japanese Undaria populations based on mitochondrial haplotypic analysis
