Molecular and morphological characterization of Digenea (Rhodomelaceae, Rhodophyta) in the Mexican Atlantic
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Martha Isabel Vilchis
,
Oscar E. Hernández
Abstract
Digenea is a widely distributed genus in the Mexican Atlantic, and until a few years ago the only morphospecies D. simplex was recorded. Recent molecular research on this species from a locality in the Mexican Caribbean revealed the new species D. mexicana, whose morphological similarity with specimens from Gulf of Mexico suggests that its distribution range could extend to this area. The aim of this work was to characterize the molecular and morphological characters of Digenea specimens from different sites in the Mexican Atlantic, to confirm this hypothesis and even reveal the presence of new species or records for the country. Our phylogenetic analysis of COI-5P and rbcL markers revealed a new record of D. arenahauriens in Mexico and confirm the presence of D. mexicana in the southern Gulf of Mexico. The species delimitation methods showed potential new species for Australia, Japan, and Brazil. The true D. simplex was not recorded molecularly for Mexico. Morphological comparisons indicated an overlap among most of the species of the genus. Future studies about molecular and morphological characterization of Digenea specimens from around the world could help to clarify the problem of specific delimitation, and even reveal new species for the genus.
About the authors
Martha Isabel Vilchis is currently a postdoctoral researcher at Universidad Autónoma de Yucatán. She is interested in biogeography, systematics, and conservation of marine algae.
Oscar E. Hernández is currently doing his PhD degree at Universidad Autónoma Metropolitana, where he is working in biogeography and systematics of marine algal species in Mexico.
Abel Sentíes is currently a professor-researcher of marine botany at Universidad Autónoma Metropolitana. He has spent over 35 years at the Marine Macroalgae Laboratory, focusing his research on systematics, biogeography, and conservation of seaweeds.
Kurt M. Dreckmann is a full-time professor at the Universidad Autonoma Metropolitana, where he received his PhD degree. For more than 35 years, his research has focused on ecology, taxonomy, biogeography, and phylogeny of marine algae.
Ileana Ortegón-Aznar is currently a full professor at Universidad Autónoma de Yucatán. She obtained her Ph.D. from Universidad Nacional Autónoma de México and has been dedicated to the study of marine macroalgae for 30 years, focusing her research on systematic, ecology and biogeography of algae.
Acknowledgements
We thank the anonymous reviewers for their valuable comments throughout the text.
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Author contributions: All the authors have actively contributed to this study and accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: OEH thanks the Consejo Nacional de Ciencia y Tecnología (CONACyT) for the scholarship granted to carry out the postgraduate studies (no. 302024). This study was supported by the Consejo Nacional de Ciencia y Tecnología through the Estancias Posdoctorales por México program, a scholarship granted to MIV (CVU: 566380). KM and AS thank for support the projects UAMI-CBS2019-2022 sesión 15.18-281118 y UAMI-CA-117, Programa para el Desarrollo Profesional Docente de la Secretaría de Educación Pública (PRODEP-SEP).
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Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this article.
References
Agardh, C.A. (1822). Species algarum rite cognitae, cum synonymis, differentiis specificis et descriptionibus succinctis. Volumen primum pars posterior. Ex officina Berlingiana, Lundae.10.5962/bhl.title.45326Search in Google Scholar
Boo, G.H., Robledo, D., Andrade-Sorcia, G., and Boo, S.M. (2018). Genetic discontinuity of Digenea (Rhodomelaceae, Rhodophyta) from Mexico supports recognition of two new species, D. mexicana and D. rafaelii. Algae 33: 231–241, https://doi.org/10.4490/algae.2018.33.8.20.Search in Google Scholar
Dawson, E.Y. (1963). Marine red algae of Pacific Mexico. Part 8. Ceramiales: Dasyaceae, Rhodomelaceae. Nova Hedwigia 6: 401–481.Search in Google Scholar
Díaz-Tapia, P., Maggs, C.A., West, J.A., and Verbruggen, H. (2017). Analysis of chloroplast genomes and a supermatrix inform reclassification of the Rhodomelaceae (Rhodophyta). J. Phycol. 53: 920–937, https://doi.org/10.1111/jpy.12553.Search in Google Scholar PubMed
Dreckmann, K.M. and Sentíes, A. (1994). El alga Digenea simplex (Ceramiales: Rhodophyta) en México: variación biogeográfica. Rev. Biol. Trop. 42: 443–453.Search in Google Scholar
Drummond, A.J., Suchard, M.A., Xie, D., and Rambaut, A. (2012). Bayesian phylogenetic with BEAUTi and the BEAST 1.7. Mol. Biol. Evol. 29: 1969–1973, https://doi.org/10.1093/molbev/mss075.Search in Google Scholar PubMed PubMed Central
Freshwater, D.W. and Rueness, J. (1994). Phylogenetics relationships of some European Gelidium (Gelidiales, Rhodophyta) species based on rbcL nucleotide sequences analysis. Phycologia 33: 187–194, https://doi.org/10.2216/i0031-8884-33-3-187.1.Search in Google Scholar
García-Soto, G. and Lopez-Bautista, J. (2018). Taxonomic notes of the genus Alsidium C. Agardh, including the merging of Bryothamnion Kützing (Rhodomelaceae). Algae 33: 215–229, https://doi.org/10.4490/algae.2018.33.6.25.Search in Google Scholar
Gouy, M., Guindon, S., and Gascuel, O. (2010). SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol. Biol. Evol. 27: 221–224, https://doi.org/10.1093/molbev/msp259.Search in Google Scholar PubMed
Guiry, M.D. and Guiry, G.M. (2022). AlgaeBase, Galway: world-wide electronic publication. National University of Ireland, Available at: <https://www.algaebase.org> (Accessed 24 January 2022).Search in Google Scholar
Leliaert, F., Verbruggen, H., Vanormelingen, P., Steen, F., López-Bautista, J.M., Zuccarello, G.C., and De Clerck, O. (2014). DNA-based species delimitation in algae. Eur. J. Phycol. 49: 179–196, https://doi.org/10.1080/09670262.2014.904524.Search in Google Scholar
Manghisi, A., Miladi, R., Minicante, S.A., Genovese, G., Le Gall, L., Abdelkafi, S., Saunders, G.W., and Morabito, M. (2019). DNA Barcoding sheds light on novel records in the Tunisia red algal flora. Cryptogam. Algol. 40: 5–27, https://doi.org/10.5252/cryptogamie-algologie2019v40a3.Search in Google Scholar
Milne, I., Lindner, D., Bayer, M., Husmeier, D., McGuire, G., Marshall, D.F., and Wright, F. (2009). TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPS clusters and multi-core desktops. Bioinformatics 25: 126–127, https://doi.org/10.1093/bioinformatics/btn575.Search in Google Scholar PubMed PubMed Central
Morrone, J.J. (2005). Hacia una síntesis biogeográfica de México. Rev. Mex. Biodivers. 76: 207–252, https://doi.org/10.22201/ib.20078706e.2005.002.303.Search in Google Scholar
Norris, R.E. (1994). Some cumophytic Rhodomelaceae (Rhodophyta) occurring in Hawaiian surf. Phycologia 33: 434–443, https://doi.org/10.2216/i0031-8884-33-6-434.1.Search in Google Scholar
Norris, J.N. (2014). Marine algae of the northern Gulf of California, II: Rhodophyta, Smithsonian Contributions to Botany 96. Smithsonian Institution Scholarly Press, Washington, D.C.10.5479/si.19382812.96Search in Google Scholar
Pons, J., Barraclough, T.G., Gómez-Zurita, J., Cardoso, A., Duran, D.P., Hazell, S., Kamoun, S., Sumlin, W.D., and Vogler, A.P. (2006). Sequences-based species delimitation for the DNA taxonomy of undescribed insects. Syst. Biol. 55: 595–609, https://doi.org/10.1080/10635150600852011.Search in Google Scholar PubMed
Puillandre, N., Lambert, A., Brouillet, S., and Achaz, G. (2012). ABGD, Automatic barcode gap discovery for primary species delimitation. Mol. Ecol. 21: 1864–1877, https://doi.org/10.1111/j.1365-294x.2011.05239.x.Search in Google Scholar PubMed
Rambaut, A., Drummond, A.J., Xie, D., Baele, G., and Suchard, M.A. (2018). Posterior summarization in Bayesian phylogenetics using Tracer v1.7. Syst. Biol. 67: 901–904, https://doi.org/10.1093/sysbio/syy032.Search in Google Scholar PubMed PubMed Central
Ronquist, F., Teslenko, M., Van der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A., and Huelsenbeck, J.P. (2012). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61: 539–542, https://doi.org/10.1093/sysbio/sys029.Search in Google Scholar PubMed PubMed Central
Saunders, G.W. (2005). Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future application. Philos. Trans. R. Soc. Lond. B Biol. Sci. 360: 1879–1888, https://doi.org/10.1098/rstb.2005.1719.Search in Google Scholar PubMed PubMed Central
Schneider, C.W., Hamzeh, B.F., Lane, C.E., and Saunders, G.W. (2018). A new species of Digenea (Rhodomelaceae, Ceramiales) based upon a molecular assessment and morphological observations of plants historically known as D. simplex in Bermuda. Phytotaxa 338: 90–98, https://doi.org/10.11646/phytotaxa.338.1.7.Search in Google Scholar
Sentíes, A., Dreckmann, K.M., Hernández, O.E., Núñez Resendiz, M.L., Le Gall, L., and Cassano, V. (2019). Diversity and distribution of Laurencia sensu stricto (Rhodomelaceae, Rhodophyta) from the Mexican Pacific, including L. mutueae sp. nov. Phycol. Res. 67: 267–278, https://doi.org/10.1111/pre.12382.Search in Google Scholar
Shimada, S., Horiguchi, T., and Masuda, M. (1999). Phylogenetic affinities of genera Acanthopeltis and Yatabella (Gelidiales, Rhodophyta) inferred from molecular analysis. Phycologia 38: 528–540, https://doi.org/10.2216/i0031-8884-38-6-528.1.Search in Google Scholar
Simons, R.H. (1970). Marine algae from southern Africa. 1. Six new species from the inter-and infra-tidal zones. Invest. Rep. Div. Sea Fish. S. Afr. 88: 1–13.Search in Google Scholar
Soares, L.P., Díaz-Tapía, P., Duran, A., Fujii, M.T., and Collado-Vides, L. (2022). Digenea nana sp. nov. (Rhodomelaceae, Rhodophyta), a new turf-forming, coral reef species from the Western Tropical Atlantic. Phycologia 61: 444–451, https://doi.org/10.1080/00318884.2022.2073422.Search in Google Scholar
Stamatakis, A. (2006). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690, https://doi.org/10.1093/bioinformatics/btl446.Search in Google Scholar PubMed
Suárez, A.M. and Martínez-Daranas, B. (2020). Similitud de la ficoflora marina en zonas del Atlántico Occidental Tropical y Subtropical. Caldasia 42: 85–95, https://doi.org/10.15446/caldasia.v42n1.73372.Search in Google Scholar
Tamura, K., Stecher, G., and Sudhir, K. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Mol. Biol. Evol. 38: 3022–3027, https://doi.org/10.1093/molbev/msab120.Search in Google Scholar PubMed PubMed Central
Vilchis, M.I., Dreckmann, K.M., García-Trejo, E.A., Hernández, O.E., and Sentíes, A. (2018). Patrones de distribución de las grandes macroalgas en el Golfo de México y el Caribe mexicano: una contribución a la biología de la conservación. Rev. Mex. Biodivers. 89: 183–192, https://doi.org/10.22201/ib.20078706e.2018.1.2226.Search in Google Scholar
Wang, H.W., Kawaguchi, S., Horiguchi, T., and Masuda, M. (2000). Reinstatement of Grateloupia catenata (Rhodophyta, Halymeniaceae) on the basis of morphology and rbcL sequences. Phycologia 39: 228–237, https://doi.org/10.2216/i0031-8884-39-3-228.1.Search in Google Scholar
Zhang, J., Kapli, P., Pavlidis, P., and Stamatakis, A. (2013). A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29: 1969–1973, https://doi.org/10.1093/bioinformatics/btt499.Search in Google Scholar PubMed PubMed Central
Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/bot-2022-0039).
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Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and Ecology
- Brazil-Malvinas Confluence in the South-West Atlantic Ocean: phytoplankton species, life forms and trophic mode
- Spatial and temporal variations of macroalgal vegetation in the north-western Red Sea
- Taxonomy/Phylogeny and Biogeography
- Phylogeographic patterns in attached and free-living marine macroalga Fucus vesiculosus (Fucaceae, Phaeophyceae) in the Baltic Sea
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- Chemistry and Applications
- Kelp nursery optimisation: density-dependent effects in early life-cycle stages of Ecklonia radiata (Laminariales)
- Corrigendum
- Corrigendum to: Morphological and molecular studies on the genus Gayralia (Ulotrichales, Chlorophyta) in northeastern Brazil with expansion of its species distribution
