Phylogenetic relationships within the genus Hypnea (Cystocloniaceae, Rhodophyta): convergent evolution and its implications in the infrageneric classification
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Priscila Barreto de Jesus
Priscila Barreto de Jesus is currently a temporary lecturer at the Department of Technology and Social Sciences (DTCS), Bahia State University (UNEB), Brazil. She was awarded a Master’s and a PhD degree in Botany from the State University of Feira de Santana, Brazil, and a postdoctoral degree from the University of São Paulo, Brazil. Her research interests are species delimitation, molecular systematics, phylogeography, phylogeny and genomics of marine macroalgae, especially the genus
Hypnea .
,
Fabio Nauer
Fabio Nauer is a postdoctoral researcher at the Botany Institute of São Paulo. PhD degree in Science, Botany sub-area, obtained at the Botany Department of the Institute of Biosciences, University of São Paulo (IB-USP), under supervision of Dr. Mariana Cabral de Oliveira. He is a specialist in the genus
Hypnea , focusing on biogeography and polyphasic taxonomy and joining molecular, morphological and eco-physiological data to understand the delimitations and distributions of the species along the Brazilian coast.José Marcos de C. Nunes has been a Professor at the Universidade Federal da Bahia (UFBA), Brazil since 1990. With a PhD in Science from the Universidade de São Paulo, Brazil (2005), his research is focused on marine algal taxonomy, systematics and ecology. He is the curator of the Cryptogam collection of the Herbarium Alexandre Leal Costa (ALCB/UFBA), and the coordinator of the Laboratório de Algas Marinhas (LAMAR).
Mariana C. Oliveira is a Professor at the University of São Paulo, Brazil. She is interested in the origin, evolution, diversity, and distribution of different groups of algae. She was on the Editorial Board of
Journal of Phycology , International Vice-President of the Phycological Society of America, Associate Editor ofPhycologia , in the Botany committee of CNPq and on the coordination committee of the Biota-FAPESP Program. Presently She is part of Area Coordination for the Biosciences of FAPESP.Alessandra Selbach Schnadelbach is an Associate Professor at Biology Institute of the Federal University of Bahia (UFBA) and coordinator of the PPG Biodiversity and Evolution. She specializes in Genetics and Plant Evolution, mainly on the following topics: genetic variability, molecular markers, phylogeny, evolution, biosystematics and genetics of populations.
Abstract
Hypnea is a monophyletic genus with a complex nomenclatural and taxonomic history, and is an important commercial source of carrageenan. Phylogenies of this genus have been accessed based primarily on Asian species; however, recent studies performed in South America revealed a great diversity of species, for which phylogenetic relationships need to be evaluated. Three infrageneric sections are recognized in the genus: Pulvinatae, Spinuligerae, and Virgatae; however, morphological and molecular circumscriptions within each section lack clarity. In this study, we analyzed three distinct markers to establish phylogenetic relationships among Hypnea species. To assign each species to the correct section, morphological data were obtained from original descriptions, reference literature, and comparisons with type/topotype and herbaria specimens. Our analyses recovered robust phylogenies for the genus and provided new insights on the taxonomic status and relationships among and within Hypnea species. The combination of three genetic markers increased the resolution and support, resulting in the largest and best-resolved phylogeny of the genus to date. Single and combined analyses revealed that the three sections of the genus Hypnea are taxonomically irrelevant, as currently recognized. Morphological differences are not associated with monophyletic groups and similarities among clades could be better explained by convergent evolution in thallus habit.
Funding source: Fundação de Amparo à Pesquisa do Estado de São Paulo
Award Identifier / Grant number: 2013/11833-3
Funding source: Fundação de Amparo à Pesquisa do Estado da Bahia
Award Identifier / Grant number: PRONEM T.O. PNE. 0020/2011
Award Identifier / Grant number: REDES T.O. RED006/2012
Funding statement: This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, BrBOL 564945/2010-2 and UNIVERSAL 477614/2013-2), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Funder Id: http://dx.doi.org/10.13039/501100001807, Biota Program, 2013/11833-3), and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB, Funder Id: http://dx.doi.org/10.13039/501100006181, PRONEM T.O. PNE. 0020/2011 and REDES T.O. RED006/2012). FN and ALC acknowledge scholarships from CNPq. IASC acknowledges scholarships from FAPESB. PBJ (150068/2017-4), JMCN (308542/2018-5), VC (302549/2017-0) and MCO (301491/2013-5) acknowledge CNPq. Many thanks to Dr. Kazuiro Kogame (SAP), Dr. Patrik Frödén (LD), Dr. Richard Searles (DUKE), Jo Wilbraham (BM), John Parnell (TCD), Dr. Heroen Verbruggen (MELU), and Dr. Daniel Robledo (MEXU) for the loan of sequences and samples.
About the authors
Priscila Barreto de Jesus is currently a temporary lecturer at the Department of Technology and Social Sciences (DTCS), Bahia State University (UNEB), Brazil. She was awarded a Master’s and a PhD degree in Botany from the State University of Feira de Santana, Brazil, and a postdoctoral degree from the University of São Paulo, Brazil. Her research interests are species delimitation, molecular systematics, phylogeography, phylogeny and genomics of marine macroalgae, especially the genus Hypnea.
Fabio Nauer is a postdoctoral researcher at the Botany Institute of São Paulo. PhD degree in Science, Botany sub-area, obtained at the Botany Department of the Institute of Biosciences, University of São Paulo (IB-USP), under supervision of Dr. Mariana Cabral de Oliveira. He is a specialist in the genus Hypnea, focusing on biogeography and polyphasic taxonomy and joining molecular, morphological and eco-physiological data to understand the delimitations and distributions of the species along the Brazilian coast.
José Marcos de C. Nunes has been a Professor at the Universidade Federal da Bahia (UFBA), Brazil since 1990. With a PhD in Science from the Universidade de São Paulo, Brazil (2005), his research is focused on marine algal taxonomy, systematics and ecology. He is the curator of the Cryptogam collection of the Herbarium Alexandre Leal Costa (ALCB/UFBA), and the coordinator of the Laboratório de Algas Marinhas (LAMAR).
Mariana C. Oliveira is a Professor at the University of São Paulo, Brazil. She is interested in the origin, evolution, diversity, and distribution of different groups of algae. She was on the Editorial Board of Journal of Phycology, International Vice-President of the Phycological Society of America, Associate Editor of Phycologia, in the Botany committee of CNPq and on the coordination committee of the Biota-FAPESP Program. Presently She is part of Area Coordination for the Biosciences of FAPESP.
Alessandra Selbach Schnadelbach is an Associate Professor at Biology Institute of the Federal University of Bahia (UFBA) and coordinator of the PPG Biodiversity and Evolution. She specializes in Genetics and Plant Evolution, mainly on the following topics: genetic variability, molecular markers, phylogeny, evolution, biosystematics and genetics of populations.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/bot-2019-0018).
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Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and ecology
- Isolate-specific resistance to the algicidal bacterium Kordia algicida in the diatom Chaetoceros genus
- Microalgal blooms in a eutrophic estuary (Golden Horn, Sea of Marmara) following a remediation effort
- Temporal succession of community structure and biphase ratio in a natural population of Ulva spp. (Chlorophyta) attached to Pyropia rafts in the Yellow Sea, China
- Taxonomy/phylogeny and biogeography
- Phylogenetic relationships within the genus Hypnea (Cystocloniaceae, Rhodophyta): convergent evolution and its implications in the infrageneric classification
- New records of the rare deep-water alga Sebdenia monnardiana (Rhodophyta) and the alien Dictyota cyanoloma (Phaeophyceae) and the unresolved case of deep-water kelp in the Ionian and Aegean Seas (Greece)
- Some rarely reported deep-water macroalgal species from Bonaire, Caribbean Sea, including Verdigellas discoidea sp. nov. (Palmophyllaceae, Chlorophyta) based on submersible collections
- On reproduction in Crouania pumila (Callithamniaceae, Rhodophyta), a new record for the Red Sea
- New record of the rare brown alga Dictyota hauckiana from Vietnam
- Solar irradiation as an alternative bleaching process for agar extracted from Gracilariopsis heteroclada in Iloilo, Philippines
- Annual reviewer acknowledgement
- Reviewer acknowledgement Botanica Marina volume 62 (2019)
Articles in the same Issue
- Frontmatter
- In this issue
- Physiology and ecology
- Isolate-specific resistance to the algicidal bacterium Kordia algicida in the diatom Chaetoceros genus
- Microalgal blooms in a eutrophic estuary (Golden Horn, Sea of Marmara) following a remediation effort
- Temporal succession of community structure and biphase ratio in a natural population of Ulva spp. (Chlorophyta) attached to Pyropia rafts in the Yellow Sea, China
- Taxonomy/phylogeny and biogeography
- Phylogenetic relationships within the genus Hypnea (Cystocloniaceae, Rhodophyta): convergent evolution and its implications in the infrageneric classification
- New records of the rare deep-water alga Sebdenia monnardiana (Rhodophyta) and the alien Dictyota cyanoloma (Phaeophyceae) and the unresolved case of deep-water kelp in the Ionian and Aegean Seas (Greece)
- Some rarely reported deep-water macroalgal species from Bonaire, Caribbean Sea, including Verdigellas discoidea sp. nov. (Palmophyllaceae, Chlorophyta) based on submersible collections
- On reproduction in Crouania pumila (Callithamniaceae, Rhodophyta), a new record for the Red Sea
- New record of the rare brown alga Dictyota hauckiana from Vietnam
- Solar irradiation as an alternative bleaching process for agar extracted from Gracilariopsis heteroclada in Iloilo, Philippines
- Annual reviewer acknowledgement
- Reviewer acknowledgement Botanica Marina volume 62 (2019)
