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Development of a tunable drug delivery system based on chitosan/polyacrylic acid polyelectrolyte multilayers from biodegradable scaffolds

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Published/Copyright: August 12, 2015
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BioNanoMaterials
From the journal BioNanoMaterials Volume 16 Issue 4

Abstract

Polyelectrolyte multilayer (PEM) films, established by layer-by-layer deposition, are attractive for drug delivery systems as drug delivery can be systematically controlled. However, one limitation of this coating method is the time-consuming character. The adsorption time of one layer typically takes minutes and commonly, investigated multilayers loaded with drugs contain more than 80 layers. Within our study we systematically evaluate parameters allowing the generation of a PEM-based drug delivery system on poly(L-lactide) (PLLA) with a reduced time consuming construction process and a controlled delivery of physiological amounts of active drugs. Therefore, several PEM based on PLLA with only five tetralayers consisting of polycation/polyanion/drug/polyanion are established and investigated regarding the release profiles of avidin fluorescein isothiocyanate as model substance and vascular endothelial growth factor (VEGF). We demonstrate that the polyelectrolyte nature and molecular weight of polyanions (hyaluronic acid, polyacrylic acid) and polycations (poly-L-lysine, chitosan) strongly influence the drug release profiles. Furthermore, in vitro cell culture studies show that the released VEGF amount and the cover layer from the favorite coating systems can beneficially enhance human umbilical vein endothelial cell (HUVEC) proliferation.

Keywords: biofunctionalization; layer-by-layer; poly- (L-lactide); vascular endothelial growth factor
Corresponding author: Dr. Katharina Wulf, Institute for Biomedical Engineering, University of Rostock, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany, Phone: +49-381-54345521, Fax: +49-381-54345602, E-mail:

Acknowledgments

The authors would like to thank Andrea Rohde, Caroline Dudda and Kim-Sarah Koek for their practical assistance. Furthermore, we thank Roswitha Dressler (University of Greifswald, Clinic for Internal Medicine B) for her excellent technical assistance. Financial support by the Bundesministerium für Bildung und Forschung (BMBF) within REMEDIS “Höhere Lebensqualität durch neuartige Mikroimplantate” (FKZ: 03IS2081) is gratefully acknowledged.

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Received: 2015-4-10
Accepted: 2015-7-23
Published Online: 2015-8-12
Published in Print: 2015-11-1

©2015 by De Gruyter

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. (Bio)Functionalization as keyfactor in implantology
  4. Special Issue Articles
  5. Microgel-functionalised fibres with pH-optimised degradation behaviour – a promising approach for short-term medical applications
  6. Development of a tunable drug delivery system based on chitosan/polyacrylic acid polyelectrolyte multilayers from biodegradable scaffolds
  7. Implications for the biofunctionalization of drug-eluting devices at the example of a site-selective antibody modification for drug eluting stents
  8. Direct sprayed endothelialization, basement membrane and cell junction development on biological and artificial products are highly substrate-dependent and require optimized biofunctionalization
  9. Endoxy – development and cultivation of textile-based gas membrane assemblies for endothelialized oxygenators
  10. Evaluation of autologous tissue sources for the isolation of endothelial cells and adipose tissue-derived mesenchymal stem cells to pre-vascularize tissue-engineered vascular grafts
https://doi.org/10.1515/bnm-2015-0011
Keywords for this article
biofunctionalization; layer-by-layer; poly- (L-lactide); vascular endothelial growth factor

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. (Bio)Functionalization as keyfactor in implantology
  4. Special Issue Articles
  5. Microgel-functionalised fibres with pH-optimised degradation behaviour – a promising approach for short-term medical applications
  6. Development of a tunable drug delivery system based on chitosan/polyacrylic acid polyelectrolyte multilayers from biodegradable scaffolds
  7. Implications for the biofunctionalization of drug-eluting devices at the example of a site-selective antibody modification for drug eluting stents
  8. Direct sprayed endothelialization, basement membrane and cell junction development on biological and artificial products are highly substrate-dependent and require optimized biofunctionalization
  9. Endoxy – development and cultivation of textile-based gas membrane assemblies for endothelialized oxygenators
  10. Evaluation of autologous tissue sources for the isolation of endothelial cells and adipose tissue-derived mesenchymal stem cells to pre-vascularize tissue-engineered vascular grafts
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