Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng Salt Lake, China
-
Xin Li
and
Ying Hui Yu
Abstract
A total of 152 halophilic bacteria were isolated from Yuncheng Salt Lake, China. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that thirty-four strains were related to the phylum Firmicutes and belonged to three families, Bacillaceae, Clostridiaceae and Staphylococcaceae. The other strains were identified as the members of Halomonadaceae and Idiomarinaceae, which belonged to the phylum γ-Proteobacteria. Nine strains showed <97% similarity of 16S rRNA gene sequence compared to other published species, which might represent novel species. The halophilic isolates exhibited various hydrolytic activities. A total of 74, 15, 70, 18, 23 and 3 strains were found to produce extracellular amylase, protease, lipase, cellulase, pectinase and DNAase, respectively. Most hydrolase-producers were members of the genus Halomonas. Combined hydrolytic activities were shown by some strains. Screening of antimicrobial activity indicated that 3, 6, 15, 12, 15 and 16 of halophilic isolates could inhibit Staphylococcus aureus, Escherichia coli, Candida albicans, Fusarium moniliforme, Fusarium semitectum and Fusarium oxysporum, respectively. Results from the present study indicated that halophilic bacteria may be developed as promising sources for novel biocatalysts or bioactive substances in the field of biotechnology.
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Articles in the same Issue
- Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng Salt Lake, China
- Prokaryotic expression and characterization of a keratinolytic protease from Aspergillus niger
- ABA-dependent sucrose regulation of antioxidant metabolism in wheat cultivars varying in ABA-sensitivity
- Identification of salt tolerant Acacia species for saline land utilisation
- Phylogenetic relationships among Elymus and related diploid genera (Triticeae: Poaceae) based on nuclear rDNA ITS sequences
- Adaptations to oxidative stress in Zea mays roots under short-term Pb2+ exposure
- Zinc alleviates cadmium effects on growth, membrane lipid biosynthesis and peroxidation in Solanum lycopersicum leaves
- Comparison of the floodplain forest floristic composition of two riparian corridors: species richness, alien species and the effect of water regime changes
- Red list of ferns and flowering plants of Slovakia, 5th edition (October 2014)
- Distribution and abundance of Hemolivia mauritanica (Apicomplexa: Haemogregarinidae) and its vector Hyalomma aegyptium in tortoises of Iran
- First record of mite genus Ledermuelleriopsis (Acari: Stigmaeidae) in South America with redescription of L. verricula from Brazil
- The influence of agricultural management on the structure of ground beetle (Coleoptera: Carabidae) assemblages
- Insect community on Jurinea cyanoides (Asteraceae), a plant species protected under NATURA 2000
- Chlorella pyrenoidosa, young barley and fruit peel polyphenols in rat breast cancer model – the effects on plasma lipid metabolism
- Increased endogenous serotonin level in diabetic conditions may lead to cardiac valvulopathy via reactive oxygen species regulation
- Parasitic helminths in grey heron (Ardea cinerea) chicks
