Cultivable bacterial flora of Indian oil reservoir: isolation, identification and characterization of the biotechnological potential
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Neha Saxena
, Soham Pore , Preeti Arora , Neelam Kapse , Anupama Engineer , Dilip R. Ranade and Prashant K. Dhakephalkar
Abstract
‘Produced water’ is a term used in oil industry to describe water produced along with oil and gas from oil reservoir. Microorganisms have been frequently isolated from produced water/oil reservoirs; however, there is paucity of information regarding the diversity and characterization of bacterial flora from Indian oil reservoirs. The present investigation was undertaken to study bacterial diversity associated with Indian oil reservoirs and to investigate their potential as a source of industrially valuable enzymes. A total of 103 strains were isolated from five oil reservoirs. PCR-based DNA fingerprinting grouped these strains into 72 genovars. These isolates were identified using morphological, phenotypical and phylogenetic analyses. Most of these isolates were thermophiles (growing at 45◦C or higher), halotolerant (growth at 5% salinity) and were distributed through a variety of genera including but not limited to Bacillus, Chelatococcus, Paenibacillus and Pseudomonas species. The 16S rRNA gene sequence of several strains shared less than 97% homology with the reference sequences in the GenBank database indicating taxonomic novelty of these strains. Assessment of the biotechnological potential of 72 genovars revealed that majority of strains produce one or many of the valuable enzymes including amylase, cellulase, xylanase, pectinase, inulinase, protease, alcohol dehydrogenase and urease. Most of the isolates also degraded crude oil or petroleum hydrocarbons. The vast pool of phenotypic, genetic and functional diversity of the strains retrieved in this study suggested oil reservoirs as yet largely untapped and potent source of taxonomically novel and biotechnologically valuable microorganisms.
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© 2015 Institute of Molecular Biology, Slovak Academy of Sciences
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Articles in the same Issue
- Cultivable bacterial flora of Indian oil reservoir: isolation, identification and characterization of the biotechnological potential
- Evaluation of the efficiency of isolated bacterial consortium PMB11 in removal of colour, degradation and reduction of toxicity from textile dye effluent
- Penicillium piceum: a potential source for antimicrobial agents and β-lactamase inhibitors
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- Retention of drug yielding potential of micropropagated Hedychium coronarium
- Occurrence and ecology of Geissleria declivis
- Genetic relationships of Chinese prickly ash as revealed by ISSR markers
- Genetic diversity of diploid Triticum species in Iran assessed using inter-retroelement amplified polymorphisms (IRAP) markers
- Diversity analysis of serpentine and non-serpentine flora – or, is serpentinite inhabited by a smaller number of species compared to different rock types?
- Systematic discovery and characterization of stress-related microRNA genes in Oryza sativa
- Two new and one already known species of the genus Thelohanellus (Myxozoa: Myxosporea: Bivalvulida) parasitizing fresh water fishes in wetlands of Punjab, India
- Palm house – biodiversity hotspot or risk of invasion? Aquatic invertebrates: The special case of Monogononta (Rotifera) under greenhouse conditions
- The effect of the invasive Asclepias syriaca on the ground-dwelling arthropod fauna
- Effect of reintroduced manual mowing on biodiversity in abandoned fen meadows
- Morphology of the only known population of Kessler’s gudgeon Romanogobio kesslerii (Teleostei: Cyprinidae) outside the Black Sea basin
- Factors predicting summer nest construction of Muscardinus avellanarius in deciduous woodland edges in Slovakia
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