Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
-
Carmen Guzmán-Cornejo
,
Luis García-Prieto
Abstract
Ornithodoros brodyi and Ornithodoros yumatensis are two species distributed in the Americas and associated with bats and caves. Both species have similar morphology, and the diagnostic traits of adults have not been detailed or illustrated accurately. In this study, the independence of both species is validated on the basis of molecular evidence (using partial sequences of 16S rDNA gene), and the morphological differences between them (dentition of the hypostome and traits of individual mammillae) are confirmed through light and scanning electron microscopy. In addition to the above characteristics, we observed other traits that may serve to differentiate both species: dorsal setae are short and thick in O. yumatensis and are thin and moderate in size in O. brodyi. We also observed a conspicuous hood in O. brodyi, which was absent in O. yumatensis. Another characteristic observed is a line of setae, near the end of Tarsi II-IV, which in O. brodyi is formed by less than five setae and in O. yumatensis by more than five. The main morphological difference between larvae of the 2 species is the number of ventral setae [9 (4 circumanal pairs) in O. brodyi and 8 (3 circumanal pairs) in O. yumatensis]. The genetic divergence in 16S rDNA sequences between these two species ranges from 9.7 to 10.6%.
Acknowledgments
We thank Laura Del Castillo Martínez for her assistance in mounting process; Silvia Espinosa Matias for preparing the scanning electron micrographs; Laura Márquez for processing samples for sequencing; Anabel Bieler Antolin for editing our photomicrographs and SEM images; Lorenza Beati for the loan of specimens from the USNTC and for allowing the use of collection facilities; Livia León Paniagua for hosts identification; Griselda Montiel-Parra, Gerardo Contreras, Josue López; Ali Lira, Martin Cabrera, Luis Darci Verde for field assistance during our biospeleological expeditions; Gerardo Rivas for statistical advice. This study was supported by the Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica, Universidad Nacional Autónoma de México (PAPIIT-UNAM No. IN214114). This manuscript was edited for proper English language by American Journal Experts editors (certificate 896F-44F5-47C5-D61C-701C).
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© 2017 W. Stefański Institute of Parasitology, PAS
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- Pseudopecoelus mccauleyi n. sp. and Podocotyle sp. (Digenea: Opecoelidae) from the deep waters off Oregon and British Columbia with an updated key to the species of Pseudopecoelus von Wicklen, 1946 and checklist of parasites from Lycodes cortezianus (Perciformes: Zoarcidae)
- New record of Steinernema arenarium (Artyukhovsky) (Rhabditida: Steinernematidae) from Ukraine and a note on its distribution
- Isolation and molecular characterization of a Naegleria strain from a recreational water fountain in Tenerife, Canary Islands, Spain
- PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks
- Three new species of Pharygodonidae (Nematoda: Oxyuridea) in Laudakia tuberculata (Squamata: Agamidae) from Dehradun, Uttarakhand, India
- Amoebicidal activity of α-bisabolol, the main sesquiterpene in chamomile (Matricaria recutita L.) essential oil against the trophozoite stage of Acanthamoeba castellani Neff
- A new species of Oochoristica (Cyclophyllidea: Linstowiidae) from non-native Mediterranean geckos, Hemidactylus turcicus (Sauria: Gekkonidae), from Texas, USA
- The ruby-crowned tanager Tachyphonus coronatus Vieillot, 1822 (Passeriformes: Thraupidae) as a new host for Isospora ramphoceli Berto, Flausino, Luz, Ferreira, Lopes, 2010 in Brazil
- Ecological implications of floods on the parasite communities of two freshwater catfishes in a Neotropical floodplain
- Morphological re-description of Electrotaenia malapteruri (Cestoda: Proteocephalidae) and Dujardinnascarismalapteruri (Nematoda: Heterocheilidae) infecting the Electric catfish Malapterurus electricus and heavy metal accumulation in host and parasites in relation to water and sediment analysis in Lake Manzala, North Delta, Egypt
- Disruption of egg production by triclabendazole-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (Mirazid)
- How does host ecology influence sampling effort in parasite diversity estimates? A case study using Neotropical freshwater fishes
- cDNA library construction of two human Demodexspecies
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- Molecular identification of Thelandros scleratus and Thelastoma icemi (Nematoda: Oxyruida) using mitochondrial cox 1 sequences
- First report on molecular evidence of Tylodelphys cerebralis (Diplostomulum cerebralis) Chakrabarti, 1968 (Digenea: Diplostomidae) from snakehead fish Channa punctata
- The role of domestic dogs in the transmission of zoonotic helminthes in a rural area of Mekong river basin
- The discovery of acanthocephalans parasitizing chaetognaths
- Redescription of Philometra margolisiMoravec, Vidal-Martínez et Aguirre-Macedo, 1995 (Nematoda: Philometridae), a gonad-infecting parasite of the red grouper Epinephelus morio (Serranidae) in the Gulf of Mexico
- Nematode parasites of the Chilean Flamingo, Phoenicopterus chilensis (Phoenicopteridae) from Central Argentina, with a description of a new species of Tetrameres (Tetrameridae)
- Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
- Morphological description of Cosmocerca sp. (Nematoda: Cosmocerdidae) from the Mascarene grass frog Ptychadena cf. mascareniensis (Amphibia: Ptychadenidae). A light and scanning electron microscopic studies
- Detection of Echinococcus multilocularis DNA in fruit, vegetable, and mushroom samples collected in the non-endemic territory of the Pomerania province and comparison of the results with data from rural areas of the neighbouring highly endemic Warmia-Masuria province, Poland
- Toxoplasma gondii in backyard pigs: seroepidemiology and mouse bioassay
- Infection of the lesser spotted dogfish with Proleptus obtusus Dujardin, 1845 (Nematoda: Spirurida) reflects ontogenetic feeding behaviour and seasonal differences in prey availability
- A new species of the quill mite genus ChenophilaKethley, 1970 (Acariformes: Syringophilidae) from the Marbled Teal Marmaronetta angustirostris (Menetries) (Anseriformes: Anatidae) in Turkey
- Neuropathological lesions of clinical and sub clinical Coenurosis (Coenurus cerebralis) in organized goat farms in India
- Case Report
- Fatal aelurostrongylosis in a kitten in Serbia
