Isolation and molecular characterization of a Naegleria strain from a recreational water fountain in Tenerife, Canary Islands, Spain
-
María Reyes-Batlle
,
Carolina Wagner
Abstract
Free-Living Amoebae (FLA) are widely distributed protozoa in the environment and have been isolated from many sources such as dust, soil and water. Among the pathogenic genera included in this group Acanthamoeba spp., Naegleria fowleri and Balamuthia mandrillaris have been reported to be causative agents of lethal encephalitis, disseminated infections and keratitis. Naegleria fowleri is a pathogenic FLA species which causes Primary Amoebic Meningoencephalitis (PAM). At present there are not many available data on the distribution of Naegleria species in Spain from environmental sources. Therefore, the aim of this study was to evaluate the presence of this genus in recreational water sources in the island of Tenerife, Canary Islands, Spain. In this study, ten samples collected from recreational water fountains were checked for the presence of Naegleria spp. using morphological and molecular identification tools. From the analysed samples, only one sample (seawater fountain) was positive for Naegleria spp. interestingly, not many reports of Naegleria spp. in seawater are available in the literature and thus awareness should be raised among the environmental and public health professionals.
Acknowledgments
This work was supported by the grants RICET (project no. RD12/0018/0012 of the programme of Redes Temáticas de Investigación Cooperativa, FIS), Spanish Ministry of Health, Madrid, Spain, the Project PI13/00490 "Protozoosis Emergentes por Amebas de Vida Libre: Aislamiento, Caracterización, Nuevas Aproximaciones Terapéuticas y Traslación Clínica de los Resultados" from the Instituto de Salud Carlos III, and Project ref. AGUA3 "Amebas de Vida Libre como Marcadores de Calidad del Agua" from Caja-Canarias Fundación. ALA was supported by a postdoctoral grant from the Fundación Canaria Manuel Morales, La Palma, Canary Islands. MRB was funded by the Project BIO24 (2016_25) "Principios activos inductores de apoptosis en la quimioterapia de tripanosomosis y leishmaniosis" from La Caixa Fundación. IS was funded by "Ayudas para estancias de estudiantes de posgrado e investigadores americanos y africanos – 2015" of the University of La Laguna. JLM was supported by the Ramón y Cajal Subprogramme from the Spanish Ministry of Economy and Competitivity RYC- 2011-08863.
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© 2017 W. Stefański Institute of Parasitology, PAS
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Articles in the same Issue
- Review
- Pseudopecoelus mccauleyi n. sp. and Podocotyle sp. (Digenea: Opecoelidae) from the deep waters off Oregon and British Columbia with an updated key to the species of Pseudopecoelus von Wicklen, 1946 and checklist of parasites from Lycodes cortezianus (Perciformes: Zoarcidae)
- New record of Steinernema arenarium (Artyukhovsky) (Rhabditida: Steinernematidae) from Ukraine and a note on its distribution
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- PCR denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in Haemaphysalis flava ticks
- Three new species of Pharygodonidae (Nematoda: Oxyuridea) in Laudakia tuberculata (Squamata: Agamidae) from Dehradun, Uttarakhand, India
- Amoebicidal activity of α-bisabolol, the main sesquiterpene in chamomile (Matricaria recutita L.) essential oil against the trophozoite stage of Acanthamoeba castellani Neff
- A new species of Oochoristica (Cyclophyllidea: Linstowiidae) from non-native Mediterranean geckos, Hemidactylus turcicus (Sauria: Gekkonidae), from Texas, USA
- The ruby-crowned tanager Tachyphonus coronatus Vieillot, 1822 (Passeriformes: Thraupidae) as a new host for Isospora ramphoceli Berto, Flausino, Luz, Ferreira, Lopes, 2010 in Brazil
- Ecological implications of floods on the parasite communities of two freshwater catfishes in a Neotropical floodplain
- Morphological re-description of Electrotaenia malapteruri (Cestoda: Proteocephalidae) and Dujardinnascarismalapteruri (Nematoda: Heterocheilidae) infecting the Electric catfish Malapterurus electricus and heavy metal accumulation in host and parasites in relation to water and sediment analysis in Lake Manzala, North Delta, Egypt
- Disruption of egg production by triclabendazole-resistant Fasciola hepatica following treatment with a commercial preparation of myrrh (Mirazid)
- How does host ecology influence sampling effort in parasite diversity estimates? A case study using Neotropical freshwater fishes
- cDNA library construction of two human Demodexspecies
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- Molecular identification of Thelandros scleratus and Thelastoma icemi (Nematoda: Oxyruida) using mitochondrial cox 1 sequences
- First report on molecular evidence of Tylodelphys cerebralis (Diplostomulum cerebralis) Chakrabarti, 1968 (Digenea: Diplostomidae) from snakehead fish Channa punctata
- The role of domestic dogs in the transmission of zoonotic helminthes in a rural area of Mekong river basin
- The discovery of acanthocephalans parasitizing chaetognaths
- Redescription of Philometra margolisiMoravec, Vidal-Martínez et Aguirre-Macedo, 1995 (Nematoda: Philometridae), a gonad-infecting parasite of the red grouper Epinephelus morio (Serranidae) in the Gulf of Mexico
- Nematode parasites of the Chilean Flamingo, Phoenicopterus chilensis (Phoenicopteridae) from Central Argentina, with a description of a new species of Tetrameres (Tetrameridae)
- Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages
- Morphological description of Cosmocerca sp. (Nematoda: Cosmocerdidae) from the Mascarene grass frog Ptychadena cf. mascareniensis (Amphibia: Ptychadenidae). A light and scanning electron microscopic studies
- Detection of Echinococcus multilocularis DNA in fruit, vegetable, and mushroom samples collected in the non-endemic territory of the Pomerania province and comparison of the results with data from rural areas of the neighbouring highly endemic Warmia-Masuria province, Poland
- Toxoplasma gondii in backyard pigs: seroepidemiology and mouse bioassay
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- Neuropathological lesions of clinical and sub clinical Coenurosis (Coenurus cerebralis) in organized goat farms in India
- Case Report
- Fatal aelurostrongylosis in a kitten in Serbia
