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Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves

  • Yuu Matsuura , Makoto Matsubayashi EMAIL logo , Satoko Nukata , Tomoyuki Shibahara , Osamu Ayukawa , Yasuko Kondo , Tomohide Matsuo , Shigehiko Uni , Masaru Furuya , Hiroyuki Tani , Naotoshi Tsuji and Kazumi Sasai
Published/Copyright: December 28, 2016
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Acta Parasitologica
From the journal Acta Parasitologica Volume 62 Issue 1

Abstract

In the production and management of beef and dairy cattle, controlling diarrhea is one of the important concerns. Pathogenic agents of the disease, protozoan parasites including Cryptosporidium spp., are difficult to control, making prevention, diagnoses, and treatment of diarrhea. In the present study, we investigated a farm with a history of calf deaths over a period of 10 years in order to determine the cause of disease and to clarify the detailed distribution of the pathogens. In four examined calves that were reared in calf pens, all were positive with Cryptosporidium and/or Giardia, while the other breeding stock and adult cattle were negative. Molecular analyses revealed that the isolates from calves were C. parvum subtype IIaA15G2R1 as a zoonotic and G. intestinalis assemblage E. Other pathogenic bacteria and diarrhea-causing viruses were not detected. After treating the calf pens with boiling water and milk of lime (Ca[OH]2), oocysts of C. parvum and cysts of G. intestinalis were not found and no additional calves died. This is the first report to describe the mixed infection of both parasites in Japan.

Keywords: Cryptosporidium parvum; genotyping; Giardia intestinalis; neonatal calves

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Received: 2016-10-21
Revised: 2016-11-23
Accepted: 2016-11-24
Published Online: 2016-12-28
Published in Print: 2017-3-1

© W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2017-0026
Keywords for this article
Cryptosporidium parvum; genotyping; Giardia intestinalis; neonatal calves

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  8. New records of host-parasite relationships between Coenagrion scitulum (Rambur, 1842) (Odonata) and water mite larvae (Hydrachnidia) in core and edge host populations
  10. A new species of myocoptid mites Myocoptes (Comyocoptes) nesokia sp. nov. (Acariformes: Myocoptidae) parasitizing Nesokia indica (Rodentia: Muridae) in Pakistan
  12. Two new species of digeneans (Lecithasteridae and Haploporidae) of the intertidal blenny Scartichthys viridis (Valenciennes) from the central coast of Chile
  14. Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients
  16. Prochristianella clarkeae Beveridge, 1990 (Eutetrarhynchidae): a species complex or a species with intraspecific variation in the distribution of its tegumental microtriches?
  18. The genetic structure of hypoderatid mites (Actinotrichida: Astigmata) parasitizing great cormorant (Phalacrocorax carbo) during host post-breeding dispersal in Milicz, SW Poland
  20. Prevalence of three Oesophagostomum spp. from Tibetan Pigs analyzed by Genetic Markers of nad1, cox3 and ITS1
  22. Anti-Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells
  24. Occurrence of Naegleria species in therapeutic geothermal water sources, Northern Iran
  26. Spermatological characteristics of the enigmatic monogenean Dictyocotyle coeliaca Nybelin, 1941 (Monopisthocotylea: Monocotylidae) reveal possible adaptation to endoparasitism
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