Home Morphological and molecular characterization of Cucullanus bourdini Petter et Le Bel, 1992 (Ascaridida: Cucullanidae) from the yellowback fusilier Caesio xanthonota Bleeker (Perciformes: Caesionidae)
Article
Licensed
Unlicensed Requires Authentication

Morphological and molecular characterization of Cucullanus bourdini Petter et Le Bel, 1992 (Ascaridida: Cucullanidae) from the yellowback fusilier Caesio xanthonota Bleeker (Perciformes: Caesionidae)

  • Zhen Xu , Hui-Xia Chen , Hui-Dong Ju and Liang Li EMAIL logo
Published/Copyright: December 28, 2016
Become an author with De Gruyter Brill
Author Information
Acta Parasitologica
From the journal Acta Parasitologica Volume 62 Issue 1

Abstract

The detailed morphology of Cucullanus bourdiniPetter et Le Bel, 1992 was further studied using both light and scanning electron microscopy, based on specimens collected from the yellowback fusilier Caesio xanthonota Bleeker (Perciformes: Caesionidae) from off the Taiwan Strait. In addition, C. bourdini was characterized genetically for the first time using molecular approaches by sequencing and analysing ribosomal [small ribosomal subunit (18S rDNA) and internal transcribed spacer 2 (ITS-2)] and mitochondrial [cytochrome c oxidase subunit 1 (cox1)] target regions. The molecular analysis of C. bourdini displayed no nucleotide differences in the 18S sequences, but detected 0.20–2.82% and 0.52–1.04% nucleotide divergence in the ITS-2 and cox1 regions, respectively. The new morphological and genetic data obtained herein would enable us to identify this hitherto little known species more rapidly and accurately.

Keywords: Ascaridida; Cucullanidae; marine fish; Caesionidae; taxonomy; molecular identification

Acknowledgements

This study was supported by the Natural Science Foundation of Hebei Province (No. C2016402118).

References

Abdel-Ghaffar F., Bashtar A.-R., Abdel-Gaber R., Morsy K., Mehlhorn H., Quraishy S.A., Mohammed S. 2014. Cucullanus egyptae sp. nov. (Nematoda, Cucullanidae) infecting the European eel Anguilla anguilla in Egypt, morphological and molecular phylogenetic studies. Parasitology Research, 113, 3457–3465. 10.1007/s00436-014-4016-4Search in Google Scholar PubMed

Caspeta-Mandujano J.M., Moravec F., Salgado-Maldonado G. 1999. Observations on cucullanid nematodes from freshwater fishes in Mexico, including Dichelyne mexicanus sp. n. Folia Parasitologica, 46, 289–295Search in Google Scholar

Floyd R.M., Rogers A.D., Lambshead J.D., Smith C.R. 2005. Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes, 5, 611–612. 10.1111/ j.1471-8286.2005.01009.xSearch in Google Scholar

Hasegawa H., Williams E.H., Bunkley-Williams L. 1991. Nematode parasites from marine fishes of Okinawa, Japan. Journal of the Helminthological Society of Washington, 58, 186–197.Search in Google Scholar

Lazarova S.S., Malloch G., Oliveira C.M.G., Hübschen J., Neilson R. 2006. Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations. Journal of Nematology, 38, 404–410Search in Google Scholar

Li L., Du L.-Q., Xu Z., Guo Y.-N., Wang S.-X., Zhang L.-P. 2014a. Morphological variability and molecular characterisation of Dichelyne (Cucullanellus) pleuronectidis (Yamaguti, 1935) (Ascaridida: Cucullanidae) from the flatfish Pleuronichthys cornutus (Temminck and Schlegel) (Pleuronectiformes: Pleuronectidae) in the East China Sea. Systematic Parasitology, 87, 87–98. 10.1007/s11230-013-9456-1Search in Google Scholar PubMed

Li L., Xu Z., Zhang L.-P. 2014b. Morphological and molecular evidence for a new species of the genus Dichelyne Jagerskiold, 1902 (Ascaridida: Cucullanidae) from marine perciform fishes in the South China Sea. Systematic Parasitology, 89, 107–116. 10.1007/s11230-014-9516-1Search in Google Scholar PubMed

Li L., Ali A.H., Zhao W.-T., Lü L., Xu Z. 2016. First report on nematode parasite infection in the yellowbar angelfish Pomacanthus maculosus (Perciformes: Pomacanthidae) from the Iraqi coral reef, with description of a new species of Cucullanus (Nematoda: Ascaridida) using the integrated approaches. Parasitology International, 65, 677–684. 10.1016/j.parint.2016.08.007Search in Google Scholar PubMed

López-Caballero J., Osorio-Sarabia D., Garcia-Prieto L. 2009. Cucullanus costaricensis n. sp. (Nematoda: Cucullanidae), a parasite of Bagre pinnimaculatus (Siluriformes: Ariidae) from Rio Tempisque, Costa Rica. Journal of Parasitology, 95, 413–423. 10.1645/GE-1682.1Search in Google Scholar PubMed

Mejia-Madrid H.H., Aguirre-Macedo M.L. 2011. Redescription and genetic characterization of Cucullanus dodsworthi (Nematoda: Cucullanidae) from the checkered puffer Sphoeroides testudineus (Pisces: Tetraodontiformes). Journal of Parasitology, 97, 695–706. 10.1645/GE-2664.1Search in Google Scholar PubMed

Miller T.L., Cribb T.H. 2007. Phylogenetic relationships of some common Indo-Pacic snappers (Perciformes: Lutjanidae) based on mitochondrial DNA sequences, with comments on the taxonomic position of the Caesioninae. Molecular Phylogenetics and Evolution, 44, 450–460. 10.1016/j.ympev.2006.10.029Search in Google Scholar PubMed

Moravec F., Justine J.L. 2011. Cucullanid nematodes (Nematoda: Cucullanidae) from deep-sea marine fishes off New Caledonia, including Dichelyne etelidis n. sp. Systematic Parasitology, 78, 95–108. 10.1007/s11230-010-9281-8Search in Google Scholar PubMed

Moravec F., Kohn A., Fernandes B.M.M. 1997. New observations on seuratoid nematodes parasitic in fishes of the Parana River, Brasil. Folia Parasitologica, 44, 209–223Search in Google Scholar

Moravec F., Sasal P., Wurtz J., Taraschewwski H. 2005. Cucullanus oceaniensis sp. n. (Nematoda: Cucullanidae) from Pacific eels (Anguilla spp.). Folia Parasitologica, 52, 343–348.10.14411/fp.2005.047Search in Google Scholar PubMed

Moravec M., Khosheghbal M., Pazooki J. 2014. Dichelyne (Dichelyne) spinigerus sp. n. (Nematoda: Cucullanidae) from the marine fish Otolithes ruber (Sciaenidae) off Iran and first description of the male of Philometra otolithi Moravec et Manoharan, 2013 (Nematoda: Philometridae). Acta Parasitologica, 59, 229–237. 10.2478/s11686-014-0228-0Search in Google Scholar PubMed

Petter A.J. 1974. Essai de classification de la famille des Cucullanidae. Bulletin du Muséum National d’Histoire Naturelle, Pairs, 3 Sér, No. 255, Zoologie, 177, 1469–1490 (In French)Search in Google Scholar

Petter A.J., Le Bel J. 1992. Two new species in the genus Cucullanus (Nematoda-Cucullanidae) from the Australian region. Memórias do Instituto Oswaldo Cruz, 87 (Suppl. I), 201–206. 10.1590/S0074-02761992000500038 TimiSearch in Google Scholar

Timi T.J., Lanfranchi A.L. 2006. A new species of Cucullanus (Nematoda: Cucullanidae) parasitizing Conger orbignianus (Pisces: Congridae) from Argentinian waters. Journal of Parasitology, 92, 151–154. 10.1645/GE-3513.1Search in Google Scholar PubMed

Xu Z., Zhang L.-P., Li L. 2014. Morphological and molecular characterization of Cucullanus hainanensis sp. nov. (Ascaridida: Cucullanidae) from Muraenichthys gymnopterus (Bleeker) (Anguilliformes: Ophichthidae) in the South China Sea. Acta Parasitologica, 59, 710–716. 10.2478/s11686-014-0303-6Search in Google Scholar PubMed

Yooyen T., Moravec F., Wongsawad C. 2011. Two new species of Cucullanus Müller, 1777 (Nematoda: Cucullanidae) from marine fishes off Thailand. Systematic Parasitology, 78, 139–149. 10.1007/s11230-010-9286-3Search in Google Scholar PubMed

Zhu X., D’Amelio S., Paggi L., Gasser R.B. 2000. Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitology Research, 86, 677–683. 10.1007/PL00008551Search in Google Scholar PubMed

Received: 2016-10-3
Revised: 2016-11-3
Accepted: 2016-11-10
Published Online: 2016-12-28
Published in Print: 2017-3-1

© 2017 W. Stefański Institute of Parasitology, PAS

Articles in the same Issue

  2. A taxonomic review of hymenolepidids (Eucestoda, Hymenolepididae) from dormice (Rodentia, Gliridae), with descriptions of two new species
  4. Trichuris suis secrete products that reduce disease severity in a multiple sclerosis model
  6. Characterization of immunoreactive proteins of Setaria cervi isolated by preparative polyacrylamide gel electrophoresis
  8. New records of host-parasite relationships between Coenagrion scitulum (Rambur, 1842) (Odonata) and water mite larvae (Hydrachnidia) in core and edge host populations
  10. A new species of myocoptid mites Myocoptes (Comyocoptes) nesokia sp. nov. (Acariformes: Myocoptidae) parasitizing Nesokia indica (Rodentia: Muridae) in Pakistan
  12. Two new species of digeneans (Lecithasteridae and Haploporidae) of the intertidal blenny Scartichthys viridis (Valenciennes) from the central coast of Chile
  14. Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients
  16. Prochristianella clarkeae Beveridge, 1990 (Eutetrarhynchidae): a species complex or a species with intraspecific variation in the distribution of its tegumental microtriches?
  18. The genetic structure of hypoderatid mites (Actinotrichida: Astigmata) parasitizing great cormorant (Phalacrocorax carbo) during host post-breeding dispersal in Milicz, SW Poland
  20. Prevalence of three Oesophagostomum spp. from Tibetan Pigs analyzed by Genetic Markers of nad1, cox3 and ITS1
  22. Anti-Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells
  24. Occurrence of Naegleria species in therapeutic geothermal water sources, Northern Iran
  26. Spermatological characteristics of the enigmatic monogenean Dictyocotyle coeliaca Nybelin, 1941 (Monopisthocotylea: Monocotylidae) reveal possible adaptation to endoparasitism
  28. Occurrence of two novel actinospore types (Cnidaria: Myxosporea) in Brazilian fish farms, and the creation of a novel actinospore collective group, Seisactinomyxon
  30. Dictyocaulosis in dairy cows in Brazil: an epidemiological, clinical-pathological and therapeutic approach
  32. Intra and inter species genetic variability of transferrin receptor gene regions in Trypanosoma evansi isolates of different livestock and geographical regions of India
  34. Transcriptional immune response in mesenteric lymph nodes in pigs with different levels of resistance to Ascaris suum
  36. The first successful report of the in vitro life cycle of Chinese Leishmania: the in vitro conversion of Leishmania amastigotes has been raised to 94% by testing 216 culture medium compound
  38. Reduced [14C]-methionine uptake and fecundity in Schistosoma mansoni females treated with recombinant tumor necrosis factor α in vitro
  40. A new feather mite of the genus Neodectes Park and Atyeo 1971 (Acari: Proctophyllodidae) from New Zealand wrens (Passeriformes: Acanthisittidae)
  42. Exploring possibilities for an alternative approach in experimental schistosomiasis mansoni: the peritoneal cavity of mice
  44. Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo
  46. Genetic diversity among Babesia rossi detected in naturally infected dogs in Abeokuta, Nigeria, based on 18S rRNA gene sequences
  48. Morphological and molecular characterization of Cucullanus bourdini Petter et Le Bel, 1992 (Ascaridida: Cucullanidae) from the yellowback fusilier Caesio xanthonota Bleeker (Perciformes: Caesionidae)
  50. Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey
  52. Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves
  53. Case Report
  54. Lethal Angiostrongylus vasorum infection in a Hungarian dog
  55. Research Note
  56. Serosurvey of anti-Toxoplasma gondii antibodies in sport horses from Paraiba state, Northeastern Brazil
  57. Erratum to: Acta Parasitologica 2016, 61(4): Table of contents
  58. Erratum to: Acta Parasitologica 2016, 61(4): Table of contents
  59. Erratum to: Acta Parasitologica (2015), 60: 354
  60. Coccidiosis in European rabbit (Oryctolagus cuniculus algirus) populations in the Iberian Peninsula
https://doi.org/10.1515/ap-2017-0024
Keywords for this article
Ascaridida; Cucullanidae; marine fish; Caesionidae; taxonomy; molecular identification

Articles in the same Issue

  2. A taxonomic review of hymenolepidids (Eucestoda, Hymenolepididae) from dormice (Rodentia, Gliridae), with descriptions of two new species
  4. Trichuris suis secrete products that reduce disease severity in a multiple sclerosis model
  6. Characterization of immunoreactive proteins of Setaria cervi isolated by preparative polyacrylamide gel electrophoresis
  8. New records of host-parasite relationships between Coenagrion scitulum (Rambur, 1842) (Odonata) and water mite larvae (Hydrachnidia) in core and edge host populations
  10. A new species of myocoptid mites Myocoptes (Comyocoptes) nesokia sp. nov. (Acariformes: Myocoptidae) parasitizing Nesokia indica (Rodentia: Muridae) in Pakistan
  12. Two new species of digeneans (Lecithasteridae and Haploporidae) of the intertidal blenny Scartichthys viridis (Valenciennes) from the central coast of Chile
  14. Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients
  16. Prochristianella clarkeae Beveridge, 1990 (Eutetrarhynchidae): a species complex or a species with intraspecific variation in the distribution of its tegumental microtriches?
  18. The genetic structure of hypoderatid mites (Actinotrichida: Astigmata) parasitizing great cormorant (Phalacrocorax carbo) during host post-breeding dispersal in Milicz, SW Poland
  20. Prevalence of three Oesophagostomum spp. from Tibetan Pigs analyzed by Genetic Markers of nad1, cox3 and ITS1
  22. Anti-Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells
  24. Occurrence of Naegleria species in therapeutic geothermal water sources, Northern Iran
  26. Spermatological characteristics of the enigmatic monogenean Dictyocotyle coeliaca Nybelin, 1941 (Monopisthocotylea: Monocotylidae) reveal possible adaptation to endoparasitism
  28. Occurrence of two novel actinospore types (Cnidaria: Myxosporea) in Brazilian fish farms, and the creation of a novel actinospore collective group, Seisactinomyxon
  30. Dictyocaulosis in dairy cows in Brazil: an epidemiological, clinical-pathological and therapeutic approach
  32. Intra and inter species genetic variability of transferrin receptor gene regions in Trypanosoma evansi isolates of different livestock and geographical regions of India
  34. Transcriptional immune response in mesenteric lymph nodes in pigs with different levels of resistance to Ascaris suum
  36. The first successful report of the in vitro life cycle of Chinese Leishmania: the in vitro conversion of Leishmania amastigotes has been raised to 94% by testing 216 culture medium compound
  38. Reduced [14C]-methionine uptake and fecundity in Schistosoma mansoni females treated with recombinant tumor necrosis factor α in vitro
  40. A new feather mite of the genus Neodectes Park and Atyeo 1971 (Acari: Proctophyllodidae) from New Zealand wrens (Passeriformes: Acanthisittidae)
  42. Exploring possibilities for an alternative approach in experimental schistosomiasis mansoni: the peritoneal cavity of mice
  44. Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo
  46. Genetic diversity among Babesia rossi detected in naturally infected dogs in Abeokuta, Nigeria, based on 18S rRNA gene sequences
  48. Morphological and molecular characterization of Cucullanus bourdini Petter et Le Bel, 1992 (Ascaridida: Cucullanidae) from the yellowback fusilier Caesio xanthonota Bleeker (Perciformes: Caesionidae)
  50. Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey
  52. Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves
  53. Case Report
  54. Lethal Angiostrongylus vasorum infection in a Hungarian dog
  55. Research Note
  56. Serosurvey of anti-Toxoplasma gondii antibodies in sport horses from Paraiba state, Northeastern Brazil
  57. Erratum to: Acta Parasitologica 2016, 61(4): Table of contents
  58. Erratum to: Acta Parasitologica 2016, 61(4): Table of contents
  59. Erratum to: Acta Parasitologica (2015), 60: 354
  60. Coccidiosis in European rabbit (Oryctolagus cuniculus algirus) populations in the Iberian Peninsula
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 23.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/ap-2017-0024/html
Scroll to top button