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Transcriptional immune response in mesenteric lymph nodes in pigs with different levels of resistance to Ascaris suum

  • Per Skallerup , Peter Nejsum , Susanna Cirera , Kerstin Skovgaard , Christian B. Pipper , Merete Fredholm , Claus B. Jørgensen and Stig M. Thamsborg EMAIL logo
Published/Copyright: December 28, 2016
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Acta Parasitologica
From the journal Acta Parasitologica Volume 62 Issue 1

Abstract

A single nucleotide polymorphism on chromosome 4 (SNP TXNIP) has been reported to be associated with roundworm (Ascaris suum) burden in pigs. The objective of the present study was to analyse the immune response to A. suum mounted by pigs with genotype AA (n = 24) and AB (n = 23) at the TXNIP locus. The pigs were repeatedly infected with A. suum from eight weeks of age until necropsy eight weeks later. An uninfected control group (AA; n = 5 and AB; n = 5) was also included. At post mortem, we collected mesenteric lymph nodes and measured the expression of 28 selected immune-related genes. Recordings of worm burdens confirmed our previous results that pigs of the AA genotype were more resistant to infection than AB pigs. We estimated the genotype difference in relative expression levels in infected and uninfected animals. No significant change in expression levels between the two genotypes due to infection was observed for any of the genes, although IL-13 approached significance (P = 0.08; Punadjusted = 0.003). Furthermore, statistical analysis testing for the effect of infection separately in each genotype showed significant up-regulation of IL-13 (P<0.05) and CCL17 (P<0.05) following A. suum infection in the ‘resistant’ AA genotype and not in the ‘susceptible’ AB genotype. Pigs of genotype AB had higher expression of the high-affinity IgG receptor (FCGR1A) than AA pigs in both infected and non-infected animals (P = 1.85*10-11).

Keywords: Ascaris suum; pig; single nucleotide polymorphism; gene expression; qPCR; RT-qPCR; cytokine; TXNIP; immunity

Acknowledgements

The authors wish to thank all those who contributed to the research project which forms the basis of this paper: Anne Strandsby, Tina Neergaard Mahler and Karin Tarp provided excellent technical assistance. We would also like to acknowledge Dries Masure for providing input to the selection of genes. P.S. is supported by a University of Copenhagen (Denmark) Ph.D. grant. This work was supported by a Danish Agency for Science, Technology and Innovation grant awarded to P.N.

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Received: 2015-12-18
Revised: 2016-9-19
Accepted: 2016-10-14
Published Online: 2016-12-28
Published in Print: 2017-3-1

© 2017 W. Stefañski Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2017-0017
Keywords for this article
Ascaris suum; pig; single nucleotide polymorphism; gene expression; qPCR; RT-qPCR; cytokine; TXNIP; immunity

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