Anti-Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and  γδ T cells

Vega Zamora; Carlos García-Ballesteros; Carmen Benet-Campos; Ferrán Ballester; Carmen Cuéllar; Juan C. Andreu-Ballester

doi:10.1515/ap-2017-0011

Article

Anti-Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells

Vega Zamora , Carlos García-Ballesteros , Carmen Benet-Campos , Ferrán Ballester , Carmen Cuéllar and Juan C. Andreu-Ballester

Published/Copyright: December 28, 2016

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From the journal Acta Parasitologica Volume 62 Issue 1

Abstract

Anisakiosis is nowadays one of the nematodoses more prevalent in Spain, with rates that oscillate between 0.43% in Galicia (N.W. Spain), and 15.7% and 22.1% in inland and southern regions, respectively. Likewise, it has been proved that Anisakis larvae have developed mechanisms to modulate the dichotomy of the host immune response for their own benefit. The experimental hypothesis of the present study was that Anisakis sp. larval products can be mediators of immune suppression and induce changes on the populations of αβ+ and γδ+ T cells. In the present study we determined the levels of anti-Anisakis antibodies in the serum of healthy people, and their relationship with the B and T cell subsets. Levels of anti-Anisakis antibodies (Ig’s, IgG, IgM, IgA and IgE) were measured by ELISA, while B and T cell subsets were studied by flow cytometry. Cells were labelled with monoclonal antibodies against CD45, CD4, CD8, CD56, CD3, CD19, TCRαβ and TCRγδ. All the specific isotypes studied were negatively correlated with NKT cell rates with the exception of IgG. A previous contact with Anisakis was related to a decrease in CD56+αβ+ and all γδ+ T cell subsets. The CD3+γδ+ population was lower in the group of subjects that showed IgA anti-Anisakis. We observed an inverse correlation among αβ-γδ NKT cells and anti-Anisakis sp. antibodies. CD3+CD56+ cells showed a significant decrease in the group of anti-Anisakis positive subjects. This fact was especially significant with CD3+CD56+γδ+ cells in the case of the anti-Anisakis IgA positive group.

Keywords: Anisakis; T cells; NKT cells; CD56+αβ+ cells; CD3+CD8+γδ+ cells; CD3+CD56+γδ+ cells

Acknowledgements

Our thanks go to Dr. AnselmoVillar-Grimalt, Head of Internal Medicine Department, for their unconditional support for our work. This study was partly supported by Fundación Ramón Areces (C.C.).

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Received: 2016-4-9

Revised: 2016-9-21

Accepted: 2016-10-10

Published Online: 2016-12-28

Published in Print: 2017-3-1

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https://doi.org/10.1515/ap-2017-0011

Keywords for this article

Anisakis; T cells; NKT cells; CD56+αβ+ cells; CD3+CD8+γδ+ cells; CD3+CD56+γδ+ cells