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Phylogenetic analysis of genetically distinct Enterocytozoon bieneusi infecting renal transplant recipients

  • Sonali Khanduja EMAIL logo , Ujjala Ghoshal and Uday C. Ghoshal
Published/Copyright: December 28, 2016
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Acta Parasitologica
From the journal Acta Parasitologica Volume 62 Issue 1

Abstract

Enterocytozoon bieneusi (E. bieneusi), infecting renal transplant (RT) recipients may be transmitted anthroponotically or zoonotically. Accordingly, we aimed to, a) evaluate genotypes of E. bieneusi ínfecting RT recipients, and b) infer phylogenetic interpretation on transmission of different genotypes among infected hosts. Stool samples of 22 RT recipients infected with microsporidia (identified using modified trichrome staining) were subjected to species identification. All E. bieneusi positive samples were subjected to genotyping. The phylogenetic tree was constructed using Mega 5 software. Of 22 microsporidia infected RT recipients, 21 (95.5%) had E. bieneusi. ITS sequences of 21 E. bieneusi were classified into eight genotypes (Ind1 to Ind8). Among them, 4 (Ind5 to Ind8) were novel. Genotypes Ind2, Ind3, Ind4, Ind7 and Ind8 showed close sequence similarity to genotypes reported exclusively from humans. Phylogenetic analysis further supported their anthroponotic transmission. Genotypes Ind1, Ind5 and Ind6 showed close sequence similarity to genotypes reported from both animals and humans. Phylogenetic analysis further supported their zoonotic transmission. Anthroponotic transmission of E. bieneusi was more common among males (11, 100% vs. 7/10, 70%; P = 0.05), presenting with diarrhea (11, 100% vs. 6/10, 60%; P = 0.02) and watery stool (10/11, 91% vs. 5/10, 50%; P = 0.03).

Keywords: Microsporidia; intestinal microsporidiosis; immune-compromised patients; internal transcribed spacer; genotyping

Acknowledgements

We would like to thank Dr. Jaco J. Verweij for providing us with the control DNA of E. bieneusi. Sonali Khanduja acknowledges the financial assistance received from Indian Council of Medical Research, Government of India through senior research fellowship grant no. 80/829/2013-ECD-I.

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Received: 2016-4-1
Revised: 2016-8-3
Accepted: 2016-9-28
Published Online: 2016-12-28
Published in Print: 2017-3-1

© W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2017-0007
Keywords for this article
Microsporidia; intestinal microsporidiosis; immune-compromised patients; internal transcribed spacer; genotyping

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