Increased level of soluble adenosine deaminase in bone marrow of visceral leishmaniasis patients: an inverse relation with parasite load

Ambak K. Rai; Prabin Kumar; Sheetal Saini; Chandreshwar P. Thakur; Tulika Seth; Dipendra K. Mitra

doi:10.1515/ap-2016-0087

Article

Increased level of soluble adenosine deaminase in bone marrow of visceral leishmaniasis patients: an inverse relation with parasite load

Ambak K. Rai , Prabin Kumar , Sheetal Saini , Chandreshwar P. Thakur , Tulika Seth and Dipendra K. Mitra

Published/Copyright: July 21, 2016

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From the journal Acta Parasitologica Volume 61 Issue 3

Abstract

Adenosine deaminase (ADA) which degrades adenosine to inosine, is known to be pro-inflammatory molecule in many diseases. Adenosine suppresses the functioning of the immune system and thus promotes dissemination of the parasite. In our previous finding, the level of soluble ADA in serum of visceral leishmaniasis (VL) was found to be increased as compared to healthy controls. However, it cannot be fairly interpreted unless their level is demonstrated at the disease site, where the parasite resides. We designed this study to correlate the level of soluble ADA (sADA) with parasitic load at the disease site i.e. bone marrow (BM). We found increased levels of sADA in BM as compared to the unaffected BM. Furthermore, a significant inverse correlation is observed between the parasite load and level of sADA at the disease site.

Keywords: Visceral leishmaniasis; bone marrow; Adenosine deaminase; Leishmania donovani

Acknowledgement

The authors thank all the patients and control patients who volunteered participation in this study. We also thank the Department of Biotechnology (Government of India) for funding the work (grant no: BT/PR6737/Med/14/871/2005). The authors thank Motilal Nehru National Institute of Technology, Allahabad and All India Institute of Medical Sciences, New Delhi for facilitating this work.

Conflict of Interest

Authors declare no conflict of interest.

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Received: 2015-9-5

Revised: 2016-2-29

Accepted: 2016-3-15

Published Online: 2016-7-21

Published in Print: 2016-9-1

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https://doi.org/10.1515/ap-2016-0087

Keywords for this article

Visceral leishmaniasis; bone marrow; Adenosine deaminase; Leishmania donovani