Redescription of Oswaldocruzia chambrieri (Strongylida: Molineidae) from Rhinella margaritifera (Anura: Bufonidae) in Caxiuanã National Forest, Brazil

Yuri Willkens; Arnaldo Maldonado; Jeannie Nascimento dos Santos; Gleomar Fabiano Maschio; Francisco Tiago de Vasconcelos Melo

doi:10.1515/ap-2016-0076

Article

Redescription of Oswaldocruzia chambrieri (Strongylida: Molineidae) from Rhinella margaritifera (Anura: Bufonidae) in Caxiuanã National Forest, Brazil

Yuri Willkens , Arnaldo Maldonado , Jeannie Nascimento dos Santos , Gleomar Fabiano Maschio and Francisco Tiago de Vasconcelos Melo

Published/Copyright: July 21, 2016

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From the journal Acta Parasitologica Volume 61 Issue 3

Abstract

Oswaldocruzia chambrieriBen Slimane et Durette-Desset, 1993 is redescribed from specimens collected from the small intestine of the South American common toad, Rhinella margaritifera, from Caxiuanã National Forest in Pará, Brazil, using light and scanning microscopy and molecular analysis of Cytochrome Oxidase I (COI) - coding regions of DNA. The discovered nematodes are characterized by a type III caudal bursa with two papillae, rays 4 with a median groove, and spicules divided into a blade, a shoe and a fork. Cervical alae are absent, the cephalic vesicle is divided into two portions, and the synlophe has low ridges without chitinous supports. The present study establishes the Caxiuanã National Forest as a new location for O. chambrieri, which had previously been reported as a parasite of R. margaritifera in Ecuador, uses light microscopy and scanning electron microscopy (SEM) to identify new morphological characters of the species and represents the second molecular sequence deposited for the Oswaldocruzia genus.

Keywords: Nematodes; Trichostrongyloidea; new morphological data; Brazilian Amazon

Acknowledgements

We are grateful to Dra. Elane Guerreiro Giese from the Laboratory of Animal Embriology and Histology from the Universidade Federal da Amazônia, Belém for their technical support with the SEM analyses. Financial support for this study was provided by the PROPESP/PPGBAIP/UFPA, CAPES Foundation, the Ministry of Education of Brazil (grant CAPES–PARASITOLOGIA BÁSICA/2010), and the National Council for Scientific and Technological Development (CNPq) (grants SISBiota 2010-Sistema Nacional de Pesquisa em Biodiversidade, PVE CAPES/CNPq A_033/2013; CNPq Research grant productivity of JNS; scientific initiation research fellowship to YWOC). This study is part of the monograph of the first author presented at Federal University of Pará, Brazil.

References

Anderson C.R, Chabaud G.A, Willmott S. 2009. Keys to the Nematode Parasites of Vertebrates, Archival Volume London: Cab International10.1079/9781845935726.0000Search in Google Scholar

Barus V., Coy Otero A. 1968. Freitasia teixerai gen. n., sp. n. and other nematodes parasitizing Anolis equestris (Squamata: Iguanidae). Folia Parasitologica, 15, 41–45Search in Google Scholar

Ben Slimane B., Chabaud A.G., Durette-Desset M. 1996. Nematodes Trichostrongylidae parasites of amphibians and reptiles: taxonomic, phylogenetic and biogeographic problems. Systematic Parasitology, 35, 179–206. DOI: 10.1007/BF00009639 (In French)Search in Google Scholar

Ben Slimane B., Durette-Desset M.C. 1993. Four new species of Oswaldocruzia Travassos, 1917 (Nematoda: Trichostrongylidae) parasites of Ecuador Amphibians. Revue Suisse de Zoologie, 100, 113–136. 9 (In French)10.5962/bhl.part.82503Search in Google Scholar

Ben Slimane B., Durette-Desset M.C., Chabaud A.G. 1995. Speciation events in Oswaldocruzia (Nematoda: Trichostrongylidae Molineidea) of Guadeloupe Lizards parasites. Parasite, 2, 373–383 (In French)Search in Google Scholar

Campião K.M., Morais D.H., Dias O.T., Aguiar A., Toledo G., Tavares L.E.R., Da Silva R.J. 2014. Checklist of helminth parasites of amphibians from South America. Zootaxa, 3843, 1–93. DOI: 10.11646/zootaxa.3843.1.1Search in Google Scholar PubMed

Dos Santos S.P., Ibáñez R., Ron S.R. 2015. Systematics of the Rhinella margaritifera complex (Anura, Bufonidae) from western Ecuador and Panamá with insights in the biogeography of Rhinella alata. ZooKeys, 501, 109–145. DOI: 10.3897/zookeys.501.8604Search in Google Scholar PubMed PubMed Central

Feitosa L.A., Furtado A.P., dos Santos J.N., Melo F.T. 2015. A new species of Kentropyxia Baker, 1982 parasitic in the small intestine of Osteocephalus taurinus Steindachner (Anura: Hylidae) from the Brazilian eastern Amazon. Systematic Parasitology, 92, 251–259. DOI: 10.1007/s11230-015-9600-1Search in Google Scholar PubMed

Frost D.R. 2014. Amphibian Species of the World: An Online Reference, Version 6.0. American Museum of Natural History, USA. Available at http://research.amnh.org/vz/herpetology/amphibia/index.html. Archived by WebCite at http://www.wecitation.org/6RVXmH2Ls on 31 March 2015Search in Google Scholar

Guerrero R. 2013. Two new species of Oswaldocruzia (Nematoda: Trichostrongylina: Molineoidea) parasites of the cane toad Rhinella marina (Amphibia: Anura) from Peru. Acta Parasitologica, 58, 30–36. DOI: 10.2478/s11686-013-0103-4Search in Google Scholar PubMed

Lavilla E.O., Caramaschi U., Langone J.A., Pombal Jr. J.P., De Sá R.O. 2013. The identity of Rana margaritifera Laurenti, 1768 (Anura, Bufonidae). Zootaxa, 3646, 251–264. DOI: 10.11646/zootaxa.3646.3.4Search in Google Scholar PubMed

Moravec F., Kaiser H. 1995. Helminth parasites from West Indian frogs, with descriptions of two new species. Caribbean Journal of Science, 31, 252–268Search in Google Scholar

Moravec J., Cusi J.C., Córdova J.H., Gvoždík V. 2014. A new species of the Rhinella margaritifera species group (Anura, Bufonidae) from the montane forest of the Selva Central, Peru. ZooKeys, 371, 35–56. DOI: 10.3897/zookeys.371.6580Search in Google Scholar PubMed PubMed Central

Pérez-Vigueras I. 1938. Notes on some new parasitic nematodes of Cuba. Livro Jubilar de Travassos, 501–508 (In Spanish)Search in Google Scholar

Prosser S.W., Velarde-Aguilar M.G., León-Règagnon V., Hebert P.D. 2013. Advancing nematode barcoding: A primer cocktail for the cytochrome coxidase subunit I gene from vertebrate parasitic nematodes. Molecular Ecology Resources, 13, 1108–1115. DOI: 10.1111/1755-0998.12082Search in Google Scholar PubMed

Santos J.N., Giese E.G., Maldonado Jr. A.J., Lanfredi R.M. 2008. A new species of Oswaldocruzia (Molineidae: Nematoda) in Chaunus marinus (Amphibian: Bufonidae) (Linneaus, 1758) from Brazil. Journal of Parasitology, 94, 264–268. DOI: 10.1645/GE-1336.1Search in Google Scholar PubMed

Svitin R., Kuzmin Y. 2012. Oswaldocruzia duboisi (Nematoda, Molineidae): morphology, hosts and distribution in Ukraine. Vestnik Zoologii, 46, 195–203. DOI: 10.2478/v10058-012-0017-xSearch in Google Scholar

Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680. DOI: 10.1093/nar/22.22.4673Search in Google Scholar PubMed PubMed Central

Travassos L. 1917. Trichostrongylidas brazileiras (5a Nota previa). Brasil Medico, 31, 1–4Search in Google Scholar

Vicente J.J, Rodrigues H.O, Gomes D.C, Pinto R.M. 1991. Nematodes in Brazil 2nd part: Amphibian Nematodes. Revista Brasileira de Zoologia, 7: 549–626 (In Portuguese)10.1590/S0101-81751990000400015Search in Google Scholar

Received: 2016-3-1

Revised: 2016-3-28

Accepted: 2016-3-31

Published Online: 2016-7-21

Published in Print: 2016-9-1

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https://doi.org/10.1515/ap-2016-0076

Keywords for this article

Nematodes; Trichostrongyloidea; new morphological data; Brazilian Amazon