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Chimeric DNA vaccines encoding Eimeria acervulina macrophage migration inhibitory factor (E.MIF) induce partial protection against experimental Eimeria infection

  • Xiaokai Song , Ruirui Zhang , Lixin Xu , Ruofeng Yan and Xiangrui Li EMAIL logo
Published/Copyright: June 16, 2015
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Acta Parasitologica
From the journal Acta Parasitologica Volume 60 Issue 3

Abstract

Chimeric DNA vaccines co-expressing Eimeria acervulina macrophage migration inhibitory factor (E.MIF) and chicken IL-2 (IL-2) or interferon-γ (IFN-γ) were constructed and their efficacies against E. acervulina were evaluated. The open reading frame (ORF) of E.MIF was cloned from E. acervulina merozoites and subcloned into the eukaryotic expression vector pVAX1 with chicken cytokine gene IFN-γ or IL-2 to construct the DNA vaccines pVAX-E.MIF-IFN-γ, pVAX-E.MIF-IL-2 and pVAX-E.MIF. The in vivo transfection of the target genes was detected by use of reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Immunizations were carried out by vaccinating chickens twice with a dose rate of 100 μg intramuscularly. Seven days post second immunization, all chickens except the unchallenged control group were challenged orally with 1 × 105 sporulated oocysts of E. acervulina. Seven days later, the duodenum was collected. The results showed that the target genes were expressed effectively in vivo. DNA vaccines and the recombinant E.MIF protein could alleviate body weight loss and duodenal lesions significantly compared to the control groups. Furthermore, pVAX-E.MIF-IL-2 and pVAX-E.MIF-IFN-γ induced anticoccidial indexs (ACIs) of 179.12 and 170, respectively, which were significantly higher than that of pVAX-E.MIF (ACI = 162.31). Our results demonstrated that E.MIF is a potential vaccine candidate against E. acervulina and chicken IFN-γ or IL- 2 may be used as genetic adjuvants to improve the efficacies of DNA vaccines against avian coccidiosis.

Keywords : E. acervulina; orthologue of macrophage migration inhibitory factor; DNA vaccine; genetic adjuvants; recombinant protein

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Received: 2015-1-5
Revised: 2015-2-10
Accepted: 2015-3-25
Published Online: 2015-6-16
Published in Print: 2015-9-1

© W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2015-0071
Keywords for this article
E. acervulina; orthologue of macrophage migration inhibitory factor; DNA vaccine; genetic adjuvants; recombinant protein

Articles in the same Issue

  1. A new species of Entobdella Blainville in Lamarck, 1818 (Monogenea: Capsalidae: Entobdellinae) from the Greenland halibut, Reinhardtius hippoglossoides
  2. Redescription and phylogenetic relationships of the rare Lyperosomum sarothrurae Baer, 1959 (Digenea: Dicrocoeliidae)
  3. Molecular epidemiology, risk factors and hematochemical alterations induced by Theileria annulata in bovines of Punjab (India)
  4. Molecular characterization of Setaria digitata from Mithun (Bos frontalis)
  5. Redescription and genetic characterization of Philometra lagocephali Moravec et Justine 2008 (Nematoda: Philometridae) from Lagocephalus lunaris (Bloch and Schneider) (Tetraodontiformes: Tetradontidae) in the South China Sea
  6. A new Cosmocercoides species (Nematoda: Cosmocercidae), C. tonkinensis n. sp., in the scale-bellied tree lizard (Acanthosaura lepidogaster) from Vietnam
  7. Ophthalmology hospital wards contamination to pathogenic free living Amoebae in Iran
  8. Eye fluke infection status in Baltic cod, Gadus morhua, after three decades and their use as ecological indicators
  9. Aplectana dubrajpuri sp. nov. (Nematoda: Cosmocercidae) in Hoplobatrachus tigerinus (Anura: Dicroglossidae) from Dubrajpur, Birbhum, West Bengal, India
  10. Trypanosoma cruzi DTU TcII presents higher blood parasitism than DTU TcI in an experimental model of mixed infection
  11. Molecular phylogeny and ultrastructure of Myxobolus cf. cuneus, a parasite of patinga hybrid and Henneguya pseudoplatystoma, a parasite of pintado hybrid
  12. Supplementary data of Henneguya leporinicola (Myxozoa, Myxosporea) a parasite of Leporinus macrocephalus from fish farms in the state of São Paulo, Brazil
  13. Prevalence and genotype of Giardia duodenalis in dairy cattle from Northern and Northeastern part of Thailand
  14. Comparison of pathogens infection level in Ips typographus (Coleoptera: Curculionidae) beetles sampled in pheromone traps and at place of overwintering
  15. A new species of Isospora (Apicomplexa: Eimeriidae) from eastern coachwhip, Coluber flagellum flagellum (Reptilia: Ophidia) from Oklahoma
  16. The first report of Trichinella pseudospiralis presence in domestic swine and T. britovi in wild boar in Bosnia and Herzegovina
  17. Metazoan parasite infracommunities of Mycteroperca bonaci (Poey, 1960) (Pisces: Epinephelidae) in reef and coastal environments off the coast of Yucatán, México
  18. Dirofilariasis – an emergent human parasitosis in Romania
  19. Two new picobiin mite species (Acari: Cheyletoidea: Syringophilidae) parasitizing passerine birds in Guyana
  20. New species of Prosotocus (Digenea; Pleurogenidae) and other helminths in Euphlyctis cyanophlyctis (Anura: Dicroglossidae) from Punjab, India
  21. Chimeric DNA vaccines encoding Eimeria acervulina macrophage migration inhibitory factor (E.MIF) induce partial protection against experimental Eimeria infection
  22. Variations in cercarial production and the level of in vitro activation of metacercariae of two different isolates of Fasciola hepatica
  23. Effect of body size on the abundance of ectoparasitic mites on the wild rodent Oligoryzomys nigripes
  24. Evaluation of reactivity to Echinococcus spp. among rural inhabitants in Poland
  25. Wild boars meat as a potential source of human trichinellosis in Poland: current data
  26. Liver fluke (Fasciola hepatica) naturally infecting introduced European brown hare (Lepus europaeus) in northern Patagonia: phenotype, prevalence and potential risk
  27. Molecular characterization of Fascioloides magna (Trematoda: Fasciolidae) from south-western Poland based on mitochondrial markers
  28. Fatal Paratanaisia bragai (Digenea: Eucotylidae) infection in scarlet macaws (Ara macao) in Costa Rica
  29. First Molecular Characterization of Aspiculuris tetraptera (Nematoda: Heteroxynematidae) from Mus musculus (Rodentia: Muridae) in India
  30. Molecular characterization of muscle-parasitizing didymozoid from a chub mackerel, Scomber japonicus
  31. Diagnosis and therapy of Capillaria plica infection: report and literature review
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