The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model
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Ayelet Livneh
Abstract
We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2-5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.
References
Arana B.A., Mendoza C.E., Rizzo N.R., Kroeger A. 2001. Randomized, controlled, double-blind trial of topical treatment of cutaneous leishmaniasis with paromomycin plus methylbenzethonium chloride ointment in Guatemala. American Journal of Tropical Medicine and Hygiene, 65, 466-470Search in Google Scholar
Arrias-Silva W.W., Collhone M.C., Ayres D.C., de Souza Souto P.C., Giorgio S. 2005. Effects of hyperbaric oxygen on Leishmania amazonensis promastigotes and amastigotes. Parasitology International, 54, 1-7Search in Google Scholar
Arrias-Silva W.W., Pinto E.F., Rossi-Bergmann B., Giorgio S. 2006. Hyperbaric oxygen therapy reduces the size of Leishmania amazonensis-induced soft tissue lesions in mice. Acta Tropica 98, 130-136Search in Google Scholar
Benson R.M., Minter L.M., Osborne B.A., Granowitz E.V. 2003. Hyperbaric oxygen inhibits stimulus-induced proinflammatory cytokine synthesis by human blood-derived monocytemacrophages. Clinical and Experimental Immunology, 13, 57-62Search in Google Scholar
Bogdan C., Moll H., Solbach W., Röllinghoff M. 1990. Tumor necrosis factor alpha in combination with interferon-gamma, but not with interleukin 4 activates murine macrophages for elimination of Leishmania major amastigotes. European Journal of Immunology, 20, 1131-1135Search in Google Scholar
Croft S.L., Yardley V. 2002. Chemotherapy of leishmaniasis. Current Pharmaceutical Design, 8, 319-342Search in Google Scholar
Desjeux P. 2004. Leishmaniasis: current situation and new perspectives. Comparative Immunology, Microbiology and Infectious Diseases, 27, 305-318 Search in Google Scholar
El-On J., Livishin R., Hamburger D., Even-Paz Z., Weinrauch L. 1986. Topical treatment of cutaneous leishmaniasis. The Journal of Investigative Dermatology, 87, 284-8Search in Google Scholar
El-On J., Halevy S., Grunwald M.H., Weinrauch L. 1992. Topical treatment of Old World cutaneous leishmaniasis caused by Leishmania major: a double-blind control study. Journal of the American Academy of Dermotology, 27, 22-31Search in Google Scholar
El-On J., Bazarsky E, Sneir R. 2007. Leishmania major: in vitro and in vivo anti-leishmanial activity of paromomycin ointment (Leshcutan) combined with the immunomodulator Imiquimod. Experimental Parasitology, 116, 156-162Search in Google Scholar
Giorgio S., Linares E., Ischiropoulos H., Von Zuben F.J., Yamada A., Augusto O. 1998. In vivo formation of electron paramagnetic resonance-detectable nitric oxide and of nitrotyrosine is not impaired during murine leishmaniasis. Infectious Immunology, 66, 807-814Search in Google Scholar
Grimaldi Jr. G., Tesh R.B. 1993. Leishmaniases of the New World: current concepts and implications for future research. Clinical Microbiology Reviews, 6, 230-250.Search in Google Scholar
Herwaldt B.L. 1999. Leishmaniasis. Lancet, 354, 1191-1199Search in Google Scholar
Knighton D.R., Silver I.A., Hunt T.K. 1981. Regulation of woundhealing angiogenesis - effect of oxygen gradients and inspired oxygen concentration. Surgery, 90, 262-270Search in Google Scholar
Kranke P, Bennett M.H., Martyn-St James M., Schnabel A., Debus S.E. 2012. Hyperbaric oxygen therapy for chronic wounds. The Chocrane Datebase of Systematic Reviews, 4, CD004123Search in Google Scholar
Labrouche S., Javorschi S., Leroy D., Gbikpi-Benissan G., Freyburger G. 1999. Influence of hyperbaric oxygen on leukocyte functions and haemostasis in normal volunteer divers. Thrombosis Research, 96, 309-315Search in Google Scholar
Lee S.A., Hasbun R. 2003. Therapy of cutaneous leishmaniasis. International Journal of Infectious Diseases, 7, 86-93Search in Google Scholar
Liew F.Y. 1989. Functional heterogeneity of CD4+ T cells in leishmaniasis. Immunology Today, 10, 40-45 Muhvich K.H., Anderson L.H., Criswell D.W., Mehm W.J. 1993. Hyperbaric hypoeroxia enhances the lethal effects of amphotericin B in Leishmania braziliensis panamensis. Undersea Hyperbaric Medicine,20, 321-328Search in Google Scholar
Navin T.R., Arana B.A., Arana F.E., Berman J.D., Chajon J.F. 1992. Placebo-controlled clinical trial of sodium stibogluconate (Pentostam) versus ketoconazole for treating cutaneous leishmaniasis in Guatemala. The Journal of Infectious Diseases 165, 528-34Search in Google Scholar
Neal R.A., Allen S., McCoy N., Olliaro P., Croft S.L. 1995. The sensitivity of Leishmania species to aminosidine. The Journal of Antimicrobial Chemotherapy, 35, 577-584Search in Google Scholar
Singer S.R., Abramson N., Shoob H., Zaken O., Zentner G., Stein- Zamir C. 2008. Ecoepidemiology of cutaneous leishmaniasis outbreak, Israel. Emerging Infectious Diseases, 14, 1424-1426Search in Google Scholar
Sitkovsky M.V., Lukashev D., Apasov S., Kojima H., Koshiba M., Caldwell C, Ohta A., ThielM. 2004. Physiological control of immune response and inflammatory tissue damage by hypoxia-inducible factors and adenosine A2A receptors. Annuual Review of Imunology, 22, 657-682Search in Google Scholar
Smith R.M., Mohideen P. 1991. One hour 1 ATA oxygen enhances rat alveolar macrophage chemiluminescence and fungal cytotoxicity. American Journal of Physiology, 260, L457-L463Search in Google Scholar
Tandara A.A., Mustoe T.A. 2004. Oxygen in wound healing - more than a nutrient. World Journal of Surgery, 28, 294-300Search in Google Scholar
Thom S.R. 2011. Hyperbaric oxygen: its mechanisms and efficacy. Plastic Reconstructive Surgery, 127 Suppl 1, 131S-141S Search in Google Scholar
© 2015 W. Stefański Institute of Parasitology, PAS
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