The transcription factor Ets-1 is expressed in human amniochorionic membranes and is up-regulated in term and preterm premature rupture of membranes
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Silké A.M. Michaelis
Abstract
Objective: The major tensile strength of fetal membranes is provided by their extracellular matrix (ECM) components. The transcription factor Ets-1 is a critical mediator of ECM remodelling. The purpose of this study was to examine whether Ets-1 is expressed in human fetal membranes and whether it is implicated in premature membrane rupture.
Study design: Amniochorionic membranes from 52 women in the following categories were analyzed for Ets-1 expression: preterm and term premature rupture of membranes, preterm and term labor and delivery, and preterm and term cesarean sections without previous onset of labor. Ets-1 protein was localized with the use of immunohistochemistry. Ets-1 levels were determined with a histoscore.
Results: Ets-1 protein was localized to the trophoblast as well as to the stromal layers. Ets-1 protein expression was up-regulated in the stroma of term and preterm prematurely ruptured membranes.
Conclusion: Ets-1 is expressed in human fetal membranes and its expression is up-regulated with premature rupture of membranes, suggesting a role for Ets-1 in ECM remodelling of the membranes.
References
1 Svigos J, J Robinson, R Vigneswaran: Prelabor rupture of the membranes. In: James D, PJ S, C Weiner, B Gonik: High Risk Pregnancy Management Opinion, W.B. Saunders, London, Edingburgh, New York, Philadelphia, Sydney, Toronto 2000Search in Google Scholar
2 Benirschke K, P Kaufmann: Placental membranes. In: Benirschke K, P Kaufmann: Pathology of the Human Placenta, Springer, New York, Heidelberg, Berlin, London, Paris, Tokyo, Hong Kong, Barcelona 199010.1007/978-1-4757-4193-3Search in Google Scholar
3 Petraglia F, P Florio, C Nappi, AR Genazzani: Peptide signaling in human placenta and membranes: autocrine, paracrine, and endocrine mechanisms. Endocr Rev17(1996) 15610.1210/edrv-17-2-156Search in Google Scholar
4 Parry S, JF Strauss 3rd: Premature rupture of the fetal membranes. N Engl J Med338 (1998) 663Search in Google Scholar
5 Skinner S, GA Campos, GC Liggins: Collagen content of human amniotic membranes: effect of gestation length and premature rupture. Obstet Gynecol57 (1981) 487Search in Google Scholar
6 Bryant-Greenwood GD: The extracellular matrix of the human fetal membranes: structure and function. Placenta19 (1998) 110.1016/S0143-4004(98)90092-3Search in Google Scholar
7 Fortunato SJ, R Menon: Distinct molecular events suggest different pathways for preterm labor and premature rupture of membranes. Am J Obstet Gynecol184 (2001) 1399Search in Google Scholar
8 Fortunato SJ, R Menon: Screening of novel matrix metalloproteinases (MMPs) in human fetal membranes. J Assist Reprod Genet19 (2002) 483Search in Google Scholar
9 Fortunato SJ, R Menon, C Bryant, SJ Lombardi: Programmed cell death (apoptosis) as a possible pathway to metalloproteinase activation and fetal membrane degradation in premature rupture of membranes. Am J Obstet Gynecol182 (2000) 1468Search in Google Scholar
10 Fortunato SJ, R Menon, SJ Lombardi: Role of tumor necrosis factor-alpha in the premature rupture of membranes and preterm labor pathways. Am J Obstet Gynecol187 (2002) 1159Search in Google Scholar
11 Malak T, C Ockleford, S Bell, R Dalgleish, N Bright, J Macvicar: Confocal immunofluorescence localization of collagen types I, III, IV, V, and VI and their ultrastructural organization in term human fetal membranes. Placenta14 (1993) 385Search in Google Scholar
12 Maymon E, R Romero, P Pacora, MT Gervasi, K Bianco, F Ghezzi, BH Yoon: Evidence for the participation of interstitial collagenase (matrix metalloproteinase 1) in preterm premature rupture of membranes. Am J Obstet Gynecol183 (2000) 914Search in Google Scholar
13 Maymon E, R Romero, P Pacora, MT Gervasi, R Gomez, SS Edwin, BH Yoon: Evidence of in vivo differential bioavailability of the active forms of matrix metalloproteinases 9 and 2 in parturition, spontaneous rupture of membranes, and intra-amniotic infection. Am J Obstet Gynecol183 (2000) 887Search in Google Scholar
14 Vadillo-Ortega F, G Gonzalez-Avila, EE Furth, H Lei, RJ Muschel, WG Stetler-Stevenson, JF 3rd Strauss: 92-kd type IV collagenase (matrix metalloproteinase-9) activity in human amniochorion increases with labor. Am J Pathol146 (1995) 148Search in Google Scholar
15 Vadillo-Ortega F, A Hernandez, G Gonzalez-Avila, L Bermejo, K Iwata, JF 3rd Strauss: Increased matrix metalloproteinase activity and reduced tissue inhibitor of metalloproteinases-1 levels in amniotic fluids from pregnancies complicated by premature rupture of membranes. Am J Obstet Gynecol174 (1996) 1371Search in Google Scholar
16 Dittmer J: The Biology of the Ets1 Proto-Oncogene. Mol Cancer2 (2003) 2910.1186/1476-4598-2-29Search in Google Scholar PubMed PubMed Central
17 Sementchenko VI, DK Watson: Ets target genes: past, present and future. Oncogene19 (2000) 6533Search in Google Scholar
18 Trojanowska M: Ets factors and regulation of the extracellular matrix. Oncogene19 (2000) 646410.1038/sj.onc.1204043Search in Google Scholar PubMed
19 Sharrocks AD: The ETS-domain transcription factor family. Nat Rev Mol Cell Biol2 (2001) 82710.1038/35099076Search in Google Scholar PubMed
20 Wernert N, AF Okuducu, MS Pepper: Ets 1 is expressed in capillary blood vessels but not in lymphatics. J Pathol200 (2003) 561Search in Google Scholar
21 Westermarck J, A Seth, VM Kahari: Differential regulation of interstitial collagenase (MMP-1) gene expression by ETS transcription factors. Oncogene14 (1997) 2651Search in Google Scholar
22 Behrens P, M Rothe, A Florin, A Wellmann, N Wernert: Invasive properties of serous human epithelial ovarian tumors are related to Ets-1, MMP-1 and MMP-9 expression. Int J Mol Med8 (2001) 149Search in Google Scholar
23 Behrens P, M Rothe, A Wellmann, J Krischler, N Wernert: The Ets-1 transcription factor is up-regulated together with MMP 1 and MMP 9 in the stroma of pre-invasive breast cancer. J Pathol194 (2001) 43Search in Google Scholar
24 The prevention of perinatal mortality and morbidity: WHO Technical Report Series. Geneva, Switzerland, World Health Organization, 1970Search in Google Scholar
25 Lionneton F, H Drobecq, F Soncin: Expression and purification of recombinant mouse Ets-1 transcription factor. Prot Expr Purif21 (2001) 492Search in Google Scholar
26 Leeman M, J McKay, G Murray: Matrix metalloproteinase 13 activity is associated with poor prognosis in colorectal cancer. J Clin Path55 (2002) 758Search in Google Scholar
27 Remmele W, U Hildebrandt, H Hienz, P Klein, M Vierbuchen, L Behnken, B Heicke, E Scheidt: Comparative histological, histochemical, immunohistochemical and biochemical studies on oestrogen receptors, lectin receptors, and Barr bodies in human breast cancer. Virchows Arch [Pathol Anat]409 (1986) 127Search in Google Scholar
28 Remmele W, HE Stegner: [Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue]. Pathologe8 (1987) 138Search in Google Scholar
29 Grevin D, JH Chen, MB Raes, D Stehelin, B Vandenbunder, X Desbiens: Involvement of the proto-oncogene c-ets 1 and the urokinase plasminogen activator during mouse implantation and placentation. Int J Dev Biol37 (1993) 519Search in Google Scholar
30 Kola I, S Brookes, AR Green, R Garber, M Tymms, TS Papas, A Seth: The Ets1 transcription factor is widely expressed during murine embryo development and is associated with mesodermal cells involved in morphogenetic processes such as organ formation. Proc Natl Acad Sci U S A90 (1993) 7588Search in Google Scholar
31 Luton D, O Sibony, JF Oury, P Blot, F Dieterlen-Lievre, L Pardanaud: The c-ets1 protooncogene is expressed in human trophoblast during the first trimester of pregnancy. Early Hum Dev47 (1997) 147Search in Google Scholar
32 Maroulakou IG, TS Papas, JE Green: Differential expression of ets-1 and ets-2 proto-oncogenes during murine embryogenesis. Oncogene9 (1994) 1551Search in Google Scholar
33 Fujimoto J, I Aoki, H Toyoki, S Khatun, E Sato, T Tamaya: Expression of ETS-1 related to angiogenesis in uterine endometrium during the menstrual cycle. J Biomed Sci10 (2003) 320Search in Google Scholar
34 Fujimoto J, Aoki I, Toyoki H, Khatun S, Tamaya T: Clinical implications of expression of ETS-1 related to angiogenesis in uterine cervical cancers. Ann Oncol13 (2002) 1598Search in Google Scholar
35 Kilpatrick LM, Kola I, Salamonsen LA: Transcription factors Ets1, Ets2, and Elf1 exhibit differential localization in human endometrium across the menstrual cycle and alternate isoforms in cultured endometrial cells. Biol Reprod61 (1999) 120Search in Google Scholar
36 Malak TM, G Mulholland, SC Bell: Morphometric characteristics of the decidua, cytotrophoblast, and connective tissue of the prelabor ruptured fetal membranes. Ann NY Acad Sci734 (1994) 430Search in Google Scholar
37 Brinckerhoff CE, Matrisian LM: Matrix metalloproteinases: a tail of a frog that became a prince. Nat Rev Mol Cell Biol3 (2002) 207Search in Google Scholar
38 Nagase H: Activation mechanisms of matrix metalloproteinases. Biol Chem378 (1997) 151Search in Google Scholar
39 Fujimoto T, S Parry, M Urbanek, M Sammel, G Macones, H Kuivaniemi, R Romero, JF 3rd Strauss: A single nucleotide polymorphism in the matrix metalloproteinase-1 (MMP-1) promoter influences amnion cell MMP-1 expression and risk for preterm premature rupture of the fetal membranes. J Biol Chem277 (2002) 6296Search in Google Scholar
40 So T, A Ito, T Sato, Y Mori, S Hirakawa: Tumor necrosis factor-alpha stimulates the biosynthesis of matrix metalloproteinases and plasminogen activator in cultured human chorionic cells. Biol Reprod46 (1992) 772Search in Google Scholar
© Walter de Gruyter
Articles in the same Issue
- The epidemiology of preterm labor – a global perspective
- Erythropoietin and prematurity – where do we stand?
- The quality of nifedipine studies used to assess tocolytic efficacy: a systematic review
- Unexplained intrauterine fetal death is accompanied by activation of complement
- Anaphylatoxins in preterm and term labor
- The transcription factor Ets-1 is expressed in human amniochorionic membranes and is up-regulated in term and preterm premature rupture of membranes
- Recombinant human lactoferrin has preventive effects on lipopolysaccharide-induced preterm delivery and production of inflammatory cytokines in mice
- Laparoscopic and vaginal repair of uterine scar dehiscence following cesarean section as detected by ultrasound
- Cerebral ventricular volume assessment by three-dimensional ultrasonography
- Nomogram of the fetal gastric size development in normal pregnancy
- Therapeutic hypothermia: from lab to NICU
- Alveolar capillary dysplasia: a six-year single center experience
- Spina bifida – a follow-up study of neonates born from 1991 to 2001
- Concordant occipital encephalocele in monoamniotic twins
- Changes of lactate, glucose, ionized calcium and glutamate concentrations in cephalic vein blood during brain hypothermia using extracorporeal membrane oxygenation (ECMO) in a newborn infant with hypoxic-ischemic encephalopathy
- Treatment of symptomatic congenital cytomegalovirus infection with valganciclovir
- A coiled nasogastric tube in a newborn
Articles in the same Issue
- The epidemiology of preterm labor – a global perspective
- Erythropoietin and prematurity – where do we stand?
- The quality of nifedipine studies used to assess tocolytic efficacy: a systematic review
- Unexplained intrauterine fetal death is accompanied by activation of complement
- Anaphylatoxins in preterm and term labor
- The transcription factor Ets-1 is expressed in human amniochorionic membranes and is up-regulated in term and preterm premature rupture of membranes
- Recombinant human lactoferrin has preventive effects on lipopolysaccharide-induced preterm delivery and production of inflammatory cytokines in mice
- Laparoscopic and vaginal repair of uterine scar dehiscence following cesarean section as detected by ultrasound
- Cerebral ventricular volume assessment by three-dimensional ultrasonography
- Nomogram of the fetal gastric size development in normal pregnancy
- Therapeutic hypothermia: from lab to NICU
- Alveolar capillary dysplasia: a six-year single center experience
- Spina bifida – a follow-up study of neonates born from 1991 to 2001
- Concordant occipital encephalocele in monoamniotic twins
- Changes of lactate, glucose, ionized calcium and glutamate concentrations in cephalic vein blood during brain hypothermia using extracorporeal membrane oxygenation (ECMO) in a newborn infant with hypoxic-ischemic encephalopathy
- Treatment of symptomatic congenital cytomegalovirus infection with valganciclovir
- A coiled nasogastric tube in a newborn