Comparative effects of chronic exposure to glucose or sodium butyrate on surfactant development in fetal rabbits
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T. D. Tshiyombo
Abstract
Background: Infants of diabetic mothers (IDM) often have delayed lung development and are thus at an increased risk of Respiratory Distress Syndrome (RDS). Both hyperglycemia and/or hyperinsulinemia have been implicated in this delay but the precise mechanism has not been clarified. Another metabolite, sodium butyrate, which is increased in IDM has been shown to decrease surfactant production in vitro but its effects on the development of the fetal lung surfactant system in vivo have not been studied.
Aim: To investigate the in vivo effects of high glucose and sodium butyrate treatment on maternal and fetal glucose and insulin levels and on fetal lung surfactant maturation using timed-pregnant New Zealand White rabbits.
Methods: On the 24th day of gestation the doe was implanted s.c. with time release pellets containing either glucose (300 mg), sodium butyrate (200 mg) or matching placebo. On the 27th or 30th day maternal (ear vein) and fetal (cardiac puncture) blood samples were drawn for glucose and insulin determinations. Fetal surfactant pools (both intra- and extracellular) were quantitatively harvested using differential and density gradient centrifugation and their phospholipid profiles determined. Data were statistically compared with ANOVA and Duncan's Multiple Range Test.
Results: Neither glucose nor sodium butyrate affected maternal plasma glucose or insulin. Both metabolites significantly increased fetal plasma insulin, decreased fetal plasma glucose but did not delay any of the parameters of surfactant maturation examined.
Conclusions: Fetal hyperinsulinemia, whether attained by prolonged exposure to elevated glucose or sodium butyrate in vivo does not appear to be the causative agent for delayed lung maturity which frequently occurs in infants of diabetic mothers.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Author Index
- Subject Index
- Contents
- Fetal and maternal energy metabolism during labor in relation to the available caloric substrate
- Incidence of meconium aspiration syndrome in term meconium-stained babies managed at birth with selective tracheal intubation
- Predicting preterm delivery and lowering very preterm delivery rate
- Comparative effects of chronic exposure to glucose or sodium butyrate on surfactant development in fetal rabbits
- Eigenvector based spatial filtering of fetal biomagnetic signals
- Localization and quantification of adhesion molecule expression in the lower uterine segment during premature labor
- Is intrapartum vibroacoustic stimulation an effective predictor of fetal acidosis?
- Plasma adrenomedullin levels in pregnancies with appropriate for gestational age and small for gestational age infants
- Tissue concentrations of cytokines in the lower uterine segment during preterm parturition
- Effect of labor on maternal dehydration, starvation, coagulation, and fibrinolysis
- Changes in light-induced fluorescence of cervical collagen in guinea pigs during gestation and after sodium nitroprusside treatment
- Cardiotocography or Doppler in making delivery decision?
- Book review
- Congress Calendar
Articles in the same Issue
- Author Index
- Subject Index
- Contents
- Fetal and maternal energy metabolism during labor in relation to the available caloric substrate
- Incidence of meconium aspiration syndrome in term meconium-stained babies managed at birth with selective tracheal intubation
- Predicting preterm delivery and lowering very preterm delivery rate
- Comparative effects of chronic exposure to glucose or sodium butyrate on surfactant development in fetal rabbits
- Eigenvector based spatial filtering of fetal biomagnetic signals
- Localization and quantification of adhesion molecule expression in the lower uterine segment during premature labor
- Is intrapartum vibroacoustic stimulation an effective predictor of fetal acidosis?
- Plasma adrenomedullin levels in pregnancies with appropriate for gestational age and small for gestational age infants
- Tissue concentrations of cytokines in the lower uterine segment during preterm parturition
- Effect of labor on maternal dehydration, starvation, coagulation, and fibrinolysis
- Changes in light-induced fluorescence of cervical collagen in guinea pigs during gestation and after sodium nitroprusside treatment
- Cardiotocography or Doppler in making delivery decision?
- Book review
- Congress Calendar
