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Detection of β-1-4-galactan in compression wood of Sitka spruce [Picea sitchensis (Bong.) Carrière] by immunofluorescence
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Clemens Altaner
Published/Copyright:
April 26, 2007
Abstract
β-1-4-Galactan has been detected in Sitka spruce [Picea sitchensis (Bong.) Carrière] by an immunofluorescence procedure. The anti-β-1-4-galactan monoclonal antibody LM5 was found to bind to compression wood (CW) tissue as identified by cell morphology features. In cross-sections, year rings with very fine bands of galactan-containing tissue were found. Cells in these bands expressed no morphological CW features. This finding was interpreted to imply that β-1-4-galactan synthesis is one of the first physiological reactions of CW formation. In cases where the CW character was pronounced, galactan was exclusively located in the outer cell-wall layers and was absent from the majority of the inner S2 layer.
Keywords: compression wood; β-1-4-galactan; immunofluorescence; monoclonal antibody (LM5); Sitka spruce [Picea sitchensis (Bong.) Carrière]
Received: 2006-8-2
Accepted: 2007-2-15
Published Online: 2007-04-26
Published in Print: 2007-5-1
©2007 by Walter de Gruyter Berlin New York
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Keywords for this article
compression wood;
β-1-4-galactan;
immunofluorescence;
monoclonal antibody (LM5);
Sitka spruce [Picea sitchensis (Bong.) Carrière]
Articles in the same Issue
- To all readers, authors and subscribers
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- A new method for the titration of free phenolic groups in pulps
- Adsorption of water-soluble organic dyes on ferrofluid-modified sawdust
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