Morphological and chemical characterisation of the G-layer in tension wood fibres of Populus tremula and Betula verrucosa: Labelling with cellulose-binding module CBM1HjCel7A and fluorescence and FE-SEM microscopy
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Geoffrey Daniel
Abstract
The gelatinous layer (G-layer) formed on the lumen wall in early- and latewood fibres of poplar and birch tension wood was characterised using a novel molecular marker specific for crystalline cellulose in conjunction with fluorescence and FE-SEM microscopy. Crystalline cellulose was localised using a cloned Cel7A cellulose-binding module (CBM1HjCel7A) from the fungus Hypocrea jecorina conjugated directly to FITC/TRITC or indirectly via a secondary antibody conjugated to FITC for fluorescence microscopy or to gold/silver for FE-SEM. With the CBM1HjCel7A conjugate, the G-layer was clearly distinguished from other secondary cell-wall layers as a bright green layer visible in fibres of tension wood in fluorescence microscopy. FEM-SEM images revealed the supramolecular architecture of the G-layer of poplar wood, which consists of well-defined, often concentrically orientated, cellulose aggregates of the order of 30–40 nm. The cellulose aggregates typically have a microfibril angle of almost 0°. Studies on cellulose marked with CBM1HjCel7A followed by Au labelling and Ag enhancement complemented the fluorescence observations. The studies demonstrate the usefulness of this novel molecular marker for crystalline cellulose in situ, which was previously difficult to localise. Further proof of distinct cellulose aggregates was observed.
References
Boraston, A.B., Bolam, D.N., Gilbert, H.J., Davies, G.J. (2004) Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem. J.382:769–781.10.1042/BJ20040892Search in Google Scholar PubMed PubMed Central
Boudet, A.M., Kajita, S., Grima-Pettenati, J., Goffner, D. (2003) Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends Plant Sci.8:576–581.10.1016/j.tplants.2003.10.001Search in Google Scholar PubMed
Côte, W.A. Jr., Day, A.C. (1962) The G-layer in gelatinous fibers-electron microscopic studies. For. Prod. J.12:333–338.Search in Google Scholar
Côte, W.A. Jr., Day, A.C. (1965) Anatomy and ultrastructure of reaction wood. In: Cellular Ultrastructure of Woody Plants. Ed. Côte W.A. Jr. Syracuse University Press. pp. 391–418.Search in Google Scholar
Daniel, G. (2003) Microview of wood under degradation by bacteria and fungi. ACS Symp. Ser.845:34–72.10.1021/bk-2003-0845.ch004Search in Google Scholar
Daniel, G., Duchesne, I. (1998). Revealing the surface ultrastructure of spruce pulp fibres using field emission-SEM. In: Proceedings of the 7th International Conference on Biotechnology in the Pulp and Paper Industry, Vancouver, Canada. pp. 81–85.Search in Google Scholar
Daniel, G., Volc, J., Niku-Paavola, M.L. (2004a) Cryo-FE-SEM and TEM immuno-techniques reveal new details for understanding white-rot decay of lignocellulose. C. R. Biol.327:861–871.10.1016/j.crvi.2004.08.003Search in Google Scholar PubMed
Daniel, G., Duchesne, I., Tokoh, C., Bardage, S. (2004b) The surface and intracellular nanostructure of wood fibres: Electron microscope methods and applications. In: Wood Fibre Cell Walls: Methods to Study their Formation, Structure and Properties. Eds. Schmitt, U., Ander, P., Barnett, J.R., Emons, A.M.C., Jeronimidis, G., Saranpää, P., Tschegg, S. SLU, Uppsala. pp. 87–104.Search in Google Scholar
Druzhinina, I., Kubicek, C.P. (2005) Species concepts and biodiversity in Trichoderma and Hypocrea: From aggregate species to species clusters? J. Zhejiang Univ. Sci. B6:100–112.10.1631/jzus.2005.B0100Search in Google Scholar PubMed PubMed Central
Duchesne, I., Daniel, G. (2000) Changes in the surface ultrastructure of Norway spruce fibres during kraft pulping – Visualization by field-emission SEM. Nord. Pulp Pap. Res. J.15:54–61.10.3183/npprj-2000-15-01-p054-061Search in Google Scholar
Duchesne, I., Hult, E.-L., Molin, U., Daniel, G., Iversen, T., Lennholm, H. (2001) The effect of hemicellulose on fibril aggregation of kraft pulp fibres as revealed by FE-SEM and CP/MAS 13C-NMR. Cellulose8:103–111.10.1023/A:1016645809958Search in Google Scholar
Duchesne, I., Takabe, K., Daniel, G. (2003) Ultrastructural localisation of glucomannan in kraft pulp fibres. Holzforschung57:62–68.10.1515/HF.2003.010Search in Google Scholar
Fengel, D., Wegener, G. Wood – Chemistry, Ultrastructure, Reactions. Walter de Gruyter, Berlin, 1984.10.1515/9783110839654Search in Google Scholar
Fernando, D., Daniel, G. (2004). Micromorphological observations on spruce TMP fibre fractions with emphasis on fibre cell wall fibrillation and splitting. Nord. Pulp Pap. J.3:278–285.Search in Google Scholar
Filonova, L., Kallas, A.M., Greffe, L., Johansson, G., Teeri, T.T., Daniel, G. (2006). Analysis of the surfaces of wood tissues and pulp fibres using carbohydrate - binding modules specific for crystalline and mannan. Biomacromolecules. Submitted for publication.Search in Google Scholar
Goto, T., Harada, H., Saiki, H. (1975) Cross-sectional view of microfibrils in gelatinous layer of poplar tension wood (Poplar euramericana). Mokuzai Gakkaishi21:537–542.Search in Google Scholar
Hildén, L., Daniel, G., Johansson, G. (2003) Use of a fluorescence labelled, carbohydrate-binding module from Phanerochaete chrysosporium Cel7D for studying wood cell wall ultrastructure. Biotechnol Lett.25:553–558.10.1023/A:1022846304826Search in Google Scholar
Hult, E.L. (2001) CP/MAS 13C-NMR spectroscopy applied to structure and interactions studies on wood and pulp fibers. Ph.D. thesis. KTH, Stockholm.Search in Google Scholar
Jervis, E.J., Haynes, C.A., Kilburn, D.G. (1997) Surface diffusion of cellulases and their isolated binding domains on cellulose. J. Biol. Chem.272:24016–24023.10.1074/jbc.272.38.24016Search in Google Scholar
Joseleau, J.P., Faix, O., Kuroda, K., Ruel, K. (2004) A polyclonal antibody directed against syringylpropane epitopes of native lignins. C. R. Biol.327:809–815.10.1016/j.crvi.2004.06.003Search in Google Scholar
Lehtiö, J., Sugiyama, J., Gustavsson, M., Fransson, L., Linder, M., Teeri, T.T. (2003) The binding specificity and affinity determinants of family 1 and family 3 cellulose binding modules. Proc. Natl. Acad. Sci. USA100:484–489.10.1073/pnas.212651999Search in Google Scholar
Linder, M., Nevanen, T., Soderholm, L., Bengs, O., Teeri, T.T. (1998) Improved immobilization of fusion proteins via cellulose-binding domains. Biotechnol. Bioeng.60:642–647.10.1002/(SICI)1097-0290(19981205)60:5<642::AID-BIT15>3.0.CO;2-8Search in Google Scholar
Linder, M., Nevanen, T., Teeri, T.T. (1999) Design of a pH-dependent cellulose-binding domain. FEBS Lett.447:13–16.10.1016/S0014-5793(99)00253-7Search in Google Scholar
McCartney, L., Gilbert, H.J., Bolam., D.N., Boraston, A.B., Knox, J.P. (2004) Glycoside hydrolase carbohydrate-binding modules as molecular probes for the analysis of plant cell wall polymers. Anal. Biochem.326:49–54.10.1016/j.ab.2003.11.011Search in Google Scholar
Mellerowicz, E.J., Baucher, M., Sundberg, B., Boerjan, W. (2001) Unravelling cell wall formation in the woody dicot stem. Plant Mol. Biol.47:239–274.10.1023/A:1010699919325Search in Google Scholar
Norberg, P.H., Meier, H. (1966) Physical and chemical properties of the gelatinous layer in tension wood fibers of aspen (Populus tremula L.). Holzforschung20:174–178.10.1515/hfsg.1966.20.6.174Search in Google Scholar
Pilate, G., Déjardin, A., Laurans, F., Leplé, J.-C. (2004) Tension wood as a model for functional genomics of wood formation. New Phytol.164:63–72.10.1111/j.1469-8137.2004.01176.xSearch in Google Scholar
Prodhan, A.K.M.A., Funada, R., Ohtani, J., Abe, H., Fukazawa, K. (1995) Orientation of microfibrils and microtubules in developing tension-wood fibres of Japanese ash (Fraxinus mandshurica var. japonica). Planta196:577–585.10.1007/BF00203659Search in Google Scholar
Ruben, G.C., Bokelman, G.H., Krakow, W. (1989) Triple-stranded left-hand helical cellulose microfibril in Acetobacter xylinium and in tobacco primary cell wall. In: Plant Cell Wall Polymers – Biogenesis and Biodegradation. Eds. Lewis, N.G., Paice, M.C. ACS, Washington. pp. 278–300.10.1021/bk-1989-0399.ch020Search in Google Scholar
Scurfield, G. (1973) Reaction wood, its structure and function. Science179:647–655.10.1126/science.179.4074.647Search in Google Scholar
Srisodsuk, M., Lehtio, J., Linder, M., Margolles-Clark, E., Reinikainen, T., Teeri, T.T. (1997) Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose. J. Biotechnol57:49–57.10.1016/S0168-1656(97)00088-6Search in Google Scholar
Taylor, J.G., Haigler, C.H., Kilburn, D.G., Blanton, R.L. (1996) Detection of cellulose with improved specificity using laser-based instruments. Biotechnol. Histochem.71:215–223.10.3109/10520299609117163Search in Google Scholar PubMed
Terashima, N., Awano, T., Takabe, K., Yoshida, M. (2004) Formation of macromolecular lignin in ginkgo xylem cell walls as observed by field emission scanning electron microscopy. C. R. Biol.327:903–910.10.1016/j.crvi.2004.08.001Search in Google Scholar PubMed
Washusen, R., Evans, R. (2001) The association between cellulose crystalline width and tension wood occurrence in Eucalyptus globulus. IAWA J.22:235–243.10.1163/22941932-90000281Search in Google Scholar
Wu, L., Joshi, C.P., Chiang, V.L. (2000) A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress. Plant J.22:495–502.10.1046/j.1365-313x.2000.00758.xSearch in Google Scholar PubMed
©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Contents
- Subject Index
- Species index (scientific names)
- Acknowledgement
- Author index
- Topochemical investigation on phenolic deposits in the vessels of afzelia (Afzelia spp.) and merbau (Intsia spp.) heartwood
- Topochemical investigations of wood extractives and their influence on colour changes in American black cherry (Prunus serotina Borkh.)
- Wound effects in the xylem of poplar: A UV microspectrophotometric study
- Application of scanning UV microspectrophotometry for the topochemical detection of lignin within individual cell walls of brown-rotted Scots pine (Pinus sylvestris L.) sapwood
- Influence of overexpression of a gibberellin 20-oxidase gene on the kinetics of xylem cell development in hybrid poplar (Populus tremula L. and P. tremuloides Michx.)
- Morphological and chemical characterisation of the G-layer in tension wood fibres of Populus tremula and Betula verrucosa: Labelling with cellulose-binding module CBM1HjCel7A and fluorescence and FE-SEM microscopy
- Decay resistance of anhydride-modified Corsican pine sapwood exposed to the brown rot fungus Coniophora puteana
- Iron-reducing capacity of low-molecular-weight compounds produced in wood by fungi
- Analysis of the hyphal load during early stages of wood decay by basidiomycetes in the presence of the wood preservative fungicides CuSO4 and cyproconazole
- Prediction of natural durability of commercial available European and Siberian larch by near-infrared spectroscopy
- Retention of Bacillus cereus and its toxin, cereulide, in cellulosic fibres
- Chemical structure elucidation of total lignins in woods. Part II: Analysis of a fraction of residual wood left after MWL isolation and solubilized in lithium chloride/N,N-dimethylacetamide
- Amphiphilicity of a lignin-carbohydrate complex
- Spatial relationships between polymers in Sitka spruce: Proton spin-diffusion studies
- Phenolic compounds from bark of Suwon poplar (Populus alba L. and Populus glandulosa Uyeki)
- Extraction of iron compounds from wood from the Vasa
- High-consistency ozonation of hardwood kraft pulp
- Fibre surface and strength of a fibre network
- A numerical study of the effects of stresses induced by moisture gradients in steel-timber dowel joints
- Pilot investigation of the mechanical properties of wood flooring paint films by in situ imaging nanoindentation
- Obituary Walter G. Kauman (1923–2005)
Articles in the same Issue
- Contents
- Subject Index
- Species index (scientific names)
- Acknowledgement
- Author index
- Topochemical investigation on phenolic deposits in the vessels of afzelia (Afzelia spp.) and merbau (Intsia spp.) heartwood
- Topochemical investigations of wood extractives and their influence on colour changes in American black cherry (Prunus serotina Borkh.)
- Wound effects in the xylem of poplar: A UV microspectrophotometric study
- Application of scanning UV microspectrophotometry for the topochemical detection of lignin within individual cell walls of brown-rotted Scots pine (Pinus sylvestris L.) sapwood
- Influence of overexpression of a gibberellin 20-oxidase gene on the kinetics of xylem cell development in hybrid poplar (Populus tremula L. and P. tremuloides Michx.)
- Morphological and chemical characterisation of the G-layer in tension wood fibres of Populus tremula and Betula verrucosa: Labelling with cellulose-binding module CBM1HjCel7A and fluorescence and FE-SEM microscopy
- Decay resistance of anhydride-modified Corsican pine sapwood exposed to the brown rot fungus Coniophora puteana
- Iron-reducing capacity of low-molecular-weight compounds produced in wood by fungi
- Analysis of the hyphal load during early stages of wood decay by basidiomycetes in the presence of the wood preservative fungicides CuSO4 and cyproconazole
- Prediction of natural durability of commercial available European and Siberian larch by near-infrared spectroscopy
- Retention of Bacillus cereus and its toxin, cereulide, in cellulosic fibres
- Chemical structure elucidation of total lignins in woods. Part II: Analysis of a fraction of residual wood left after MWL isolation and solubilized in lithium chloride/N,N-dimethylacetamide
- Amphiphilicity of a lignin-carbohydrate complex
- Spatial relationships between polymers in Sitka spruce: Proton spin-diffusion studies
- Phenolic compounds from bark of Suwon poplar (Populus alba L. and Populus glandulosa Uyeki)
- Extraction of iron compounds from wood from the Vasa
- High-consistency ozonation of hardwood kraft pulp
- Fibre surface and strength of a fibre network
- A numerical study of the effects of stresses induced by moisture gradients in steel-timber dowel joints
- Pilot investigation of the mechanical properties of wood flooring paint films by in situ imaging nanoindentation
- Obituary Walter G. Kauman (1923–2005)
