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Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls

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Published/Copyright: June 1, 2005
Holzforschung
From the journal Volume 55 Issue 6

Summary

The localisation of lignin and phenolic extractives in woody tissue was determined using scanning UV microspectrophotometry. This improved cellular analytical technique enabled direct imaging of the topochemical lignin distribution within individual cell wall layers with a resolution of 0.25 μm2. Selected softwood (Picea abies), hardwood (Fagus sylvatica, Entandrophragma cylindricum, Prunus serotina) and monocotyledon (Phyllostachys edulis) sections of 1 μm thickness were scanned at a fixed wavelength and evaluated with the “APAMOS” software. This approach allowed the distribution pattern of lignins and aromatic extractives within the cell wall to be visualised simultaneously. The method was found to be ideally suited to the study of their subcellular distribution in plant cell walls.

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Published Online: 2005-06-01
Published in Print: 2001-11-06

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls
  2. Fungicidal Activity of 3'-Substituted-3-Stilbenols
  3. Characterisation of a Soft Rot-Like Decay Pattern Caused by Coniophora puteana (Schum.) Karst. in Sapelli Wood (Entandrophragma cylindricum Sprague)
  4. Transformation of Chlorophenols by White-Rot Fungi and their Laccase
  5. Fixation of Amine Copper Preservatives. Part 1. Reaction of Vanillin, a Lignin Model Compound with Monoethanolamine Copper Sulphate Solution
  6. Influence of Moisture Sorption on Swelling of Mahogany (Swietenia macrophylla King) Wood
  7. Silica in Several Rattan Species
  8. Changes in the Properties of Light-Irradiated Wood with Heat Treatment. Part 1. Effect of Treatment Conditions on the Change in Color
  9. Evaluating Strength Properties of Pinus radiata from Ultrasonic Measurements on Increment Cores
  10. Delignification Mechanism during High-Boiling Solvent Pulping. Part 1. Reaction of Guaiacylglycerol-β-Guaiacyl Ether
  11. Liquefaction Mechanism of β-O-4 Lignin Model Compound in the Presence of Phenol under Acid Catalysis. Part 1. Identification of the Reaction Products
  12. Liquefaction Mechanism of β-O-4 Lignin Model Compound in the Presence of Phenol under Acid Catalysis. Part 2. Reaction Behaviour and Pathways
  13. Study of Residual Lignin in Pulp by UV Resonance Raman Spectroscopy
  14. The Importance of Xylan for the Strength Properties of Spruce Kraft Pulp Fibres
  15. Eucalyptus globulus Kraft Pulp Residual Lignin. Part 2. Modification of Residual Lignin Structure in Oxygen Bleaching
  16. Monitoring Acoustic Emissions from Finger-Joints from Tropical African Hardwoods for Predicting Ultimate Tensile Strength
  17. Hydrolytic Processes and Condensation Reactions in the Cellulose Solvent System N,N-Dimethylacetamide/Lithium Chloride. Part 1.
  18. Viscoelastic Properties of Liquefied Wood/Epoxy Resin and its Bond Strength
  19. Prof. Dr. Josef Bauch 65 years old
  20. In Memory of Prof. Guglielmo Giordano
  21. Literature Reports
https://doi.org/10.1515/HF.2001.091

Articles in the same Issue

  1. Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls
  2. Fungicidal Activity of 3'-Substituted-3-Stilbenols
  3. Characterisation of a Soft Rot-Like Decay Pattern Caused by Coniophora puteana (Schum.) Karst. in Sapelli Wood (Entandrophragma cylindricum Sprague)
  4. Transformation of Chlorophenols by White-Rot Fungi and their Laccase
  5. Fixation of Amine Copper Preservatives. Part 1. Reaction of Vanillin, a Lignin Model Compound with Monoethanolamine Copper Sulphate Solution
  6. Influence of Moisture Sorption on Swelling of Mahogany (Swietenia macrophylla King) Wood
  7. Silica in Several Rattan Species
  8. Changes in the Properties of Light-Irradiated Wood with Heat Treatment. Part 1. Effect of Treatment Conditions on the Change in Color
  9. Evaluating Strength Properties of Pinus radiata from Ultrasonic Measurements on Increment Cores
  10. Delignification Mechanism during High-Boiling Solvent Pulping. Part 1. Reaction of Guaiacylglycerol-β-Guaiacyl Ether
  11. Liquefaction Mechanism of β-O-4 Lignin Model Compound in the Presence of Phenol under Acid Catalysis. Part 1. Identification of the Reaction Products
  12. Liquefaction Mechanism of β-O-4 Lignin Model Compound in the Presence of Phenol under Acid Catalysis. Part 2. Reaction Behaviour and Pathways
  13. Study of Residual Lignin in Pulp by UV Resonance Raman Spectroscopy
  14. The Importance of Xylan for the Strength Properties of Spruce Kraft Pulp Fibres
  15. Eucalyptus globulus Kraft Pulp Residual Lignin. Part 2. Modification of Residual Lignin Structure in Oxygen Bleaching
  16. Monitoring Acoustic Emissions from Finger-Joints from Tropical African Hardwoods for Predicting Ultimate Tensile Strength
  17. Hydrolytic Processes and Condensation Reactions in the Cellulose Solvent System N,N-Dimethylacetamide/Lithium Chloride. Part 1.
  18. Viscoelastic Properties of Liquefied Wood/Epoxy Resin and its Bond Strength
  19. Prof. Dr. Josef Bauch 65 years old
  20. In Memory of Prof. Guglielmo Giordano
  21. Literature Reports
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