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The evaluation of colloidal gold immunochromatographic assay (GICA) for rapid diagnosis of influenza A disease

  • Xuehui Li , Hangwei Chen EMAIL logo , Juan Wei , Nan Lv and Lanhua You
Published/Copyright: June 2, 2011
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Clinical Chemistry and Laboratory Medicine
From the journal Clinical Chemistry and Laboratory Medicine Volume 49 Issue 9

Abstract

Background: A low cost colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A virus was developed. The assay was evaluated in this study.

Methods: Six hundred and twenty-six patients were enrolled. All patients contributed two pharyngeal swabs, one used for colloidal gold immunochromatographic rapid assay for influenza A virus immediately after the collection of specimen and one used for real-time reverse transcriptase polymerase chain reaction (RT-PCR) test or virus culture at the Centers for Disease Control and Prevention (CDC) influenza network laboratory.

Result: In reference to viral culture, GICA influenza A test demonstrated a sensitivity of 64%, a specificity of 95% and an overall accuracy of 93%. Consistency between the GICA test and virus culture assay is moderate, with Kappa being 0.46. In reference to RT-PCR, GICA test demonstrated considerable high sensitivity (74%) and specificity (86%), with Kappa value being 0.61 and overall accuracy of 81%. There was no significant difference between GICA test and virus culture/RT-PCR on the detected positive rates of influenza A cases.

Conclusions: GICA is a reliable, rapid, convenient and inexpensive test for the screening and diagnosis of influenza A disease. Given its lower cost than other rapid tests, the GICA test has the great potential in the management of influenza A disease in resource-poor countries.

Keywords: colloidal gold immunochromatographic assay; influenza A; reverse transcriptase polymerase chain reaction (RT-PCR); virus culture
Corresponding author: Hangwei Chen, MD, PhD, Department of Respiratory Medicine, The Military General Hospital of Beijing PLA, No. 5, Nanmencang, Dongcheng District, Beijing 100700, P.R. China Phone/Fax: +86-10-66721167
Received: 2010-12-30
Accepted: 2011-4-20
Published Online: 2011-06-2
Published in Print: 2011-09-01

©2011 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/CCLM.2011.235
Keywords for this article
colloidal gold immunochromatographic assay; influenza A; reverse transcriptase polymerase chain reaction (RT-PCR); virus culture

Articles in the same Issue

  1. Editorial
  2. Athlete's biological passport: to test or not to test?
  3. Review
  4. Prognostic value of cystatin C in acute coronary syndromes: enhancer of atherosclerosis and promising therapeutic target
  5. Opinion Papers
  6. The function of oxalic acid in the human metabolism
  7. Current limitations of the Athlete's Biological Passport use in sports
  8. Limits and pitfalls of Athlete's Biological Passport
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  10. Perspectives
  11. Genetics of infectious diseases: hidden etiologies and common pathways
  12. Guidelines and Recommendations
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  14. Genetics and Molecular Diagnostics
  15. A template for mutational data analysis of the CFTR gene
  16. High resolution melting analysis to genotype the most common variants in the HFE gene
  17. General Clinical Chemistry and Laboratory Medicine
  18. Bayesian analysis of an international ELISA comparability study
  19. Inflammatory markers in preeclamptic patients
  20. Pre-analytical effects of different lithium heparin plasma separation tubes in the routine clinical chemistry laboratory
  21. Pre-acquisition system assessment of the Sysmex® Coagulation System CS-2100i and comparison with end-user verification; a model for the regional introduction of new analysers and methods
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  26. Determinants of oxidative stress related to gender: relevance of age and smoking habit
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  30. Prognostic value of serum angiotensin-converting enzyme activity for outcome of community-acquired pneumonia
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  32. Cardiovascular Disease
  33. N-terminal pro-B-type natriuretic peptide in early and advanced phases of obesity
  34. Vaspin plasma concentrations and mRNA expressions in patients with stable and unstable angina pectoris
  35. Prospective study of first stroke in relation to plasma homocysteine and MTHFR 677C>T and 1298A>C genotypes and haplotypes – evidence for an association with hemorrhagic stroke
  36. Letters to the Editor
  37. Evaluation of the analytical performance of the Beckman Coulter AU680 automated analytical system based on quality specifications for allowable performance derived from biological variation
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  39. Erratum
  40. Modified phosphatidylserine-dependent antiprothrombin ELISA enables identification of patients negative for other antiphospholipid antibodies and also detects low avidity antibodies
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