Article
Licensed
Unlicensed
Requires Authentication
Calcium pyrophosphate and monosodium urate crystals in synovial fluid as a cause of pseudoeosinophilia
-
Christoph Robier
Published/Copyright:
May 31, 2011
Abstract
Background: Synovial fluid (SF) leukocytes can be counted microscopically in a Neubauer chamber or by automated procedures using haematology analysers. Knowledge of laboratory artefacts is crucial for the correct interpretation of results obtained using automated methods. SF pseudoeosinophilia has recently been described as a new artefact in patients with crystal-related arthropathies. We investigated whether pseudoeosinophilia of SF is restricted to crystal-related disorders, or if it may also occur in other arthropathies.
Methods: We compared the percentages of eosinophils in 120 crystal containing SF samples with 185 crystal-free specimens using the Wilcoxon test. Crystal positive samples, determined by polarised microscopy, contained at least two monosodium urate or calcium pyrophosphate crystals per 10 high power fields (630× magnification). True SF eosinophilia was ruled out by microscopic examination of stained slides.
Results: Crystal positive samples had significantly higher percentages of eosinophils than the controls (p<0.0001). No significant differences between the two crystal types were found (p=0.693). Thus, pseudoeosinophilia was significantly correlated with the presence of crystals, and none of the distinct crystal types was more likely to be associated with pseudoeosinophilia.
Conclusions: In this study, SF pseudoeosinophilia was confirmed as a crystal-related laboratory artefact which has to be considered in the interpretation of automated SF leukocyte differential counts.
Received: 2011-01-10
Accepted: 2011-03-13
Published Online: 2011-05-31
Published in Print: 2011-08-01
©2011 by Walter de Gruyter Berlin Boston
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorial
- Pneumatic tube delivery systems for patient samples: evidence of quality and quality of evidence
- Reviews
- Translating pharmacogenetics into clinical practice: interleukin (IL)28B and inosine triphosphatase (ITPA) polymophisms in hepatitis C virus (HCV) infection
- New biomarkers for acute renal injury
- Minireview
- Post-mortem biochemistry of vitreous humor and glucose metabolism: an update
- Opinion Paper
- International Osteoporosis Foundation and International Federation of Clinical Chemistry and Laboratory Medicine Position on bone marker standards in osteoporosis
- Guidelines and Recommendations
- IFCC international conventional reference procedure for the measurement of free thyroxine in serum
- Genetics and Molecular Diagnostics
- Improving clinical laboratory efficiency: a time-motion evaluation of the Abbott m2000 RealTime and Roche COBAS AmpliPrep/COBAS TaqMan PCR systems for the simultaneous quantitation of HIV-1 RNA and HCV RNA
- Enhanced frequency of CFTR gene variants in couples who are candidates for assisted reproductive technology treatment
- Customer satisfaction survey to improve the European cystic fibrosis external quality assessment scheme
- General Clinical Chemistry and Laboratory Medicine
- Effect of sample collection, temperature and time of storage on β-galactosidase and total hexosaminidase activities in dried blood collected on filter paper
- Agreement between paired blood gas values in samples transported either by a pneumatic system or by human courier
- Golgi protein 73(GP73), a useful serum marker in liver diseases
- The utility of six over-the-counter (home) pregnancy tests
- N Latex FLC – new monoclonal high-performance assays for the determination of free light chain kappa and lambda
- Alterations of the preoperative coagulation profile in patients with acute appendicitis
- Cerebrospinal fluid findings in infants with pertussis or parapertussis1)
- Calcium pyrophosphate and monosodium urate crystals in synovial fluid as a cause of pseudoeosinophilia
- Cardiovascular Disease
- Dual activity of serum lipoprotein-associated phospholipase A2 yielding positive and inverse associations with cardiometabolic risk
- The value of N-terminal proB-type natriuretic peptide for early identification of myocardial infarction in patients with high-risk non-ST-elevation acute coronary syndromes
- Infectious Diseases
- Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)
- Evaluation of two automated chemiluminescence immunoassays, the LIAISON Treponema Screen and the ARCHITECT Syphilis TP, and the Treponema pallidum particle agglutination test for laboratory diagnosis of syphilis
- Letters to the Editor
- The preanalytical influence of two different mechanical transport systems on laboratory analysis
- Commutability of the ERM-DA470k Reference Material for two assays measuring serum albumin using immunochemical principles
- Performance evaluation of the Vitros®3600 immunodiagnostic system for the determination of free thyroid hormones
- Towards laboratory knowledge, not data, in 70% of clinical decision-making. What “knowledge management” can add to clinical practice?
- How to obtain DNA from injection drug users?
Keywords for this article
calcium pyrophosphate;
eosinophilia;
monosodium urate;
pseudoeosinophilia;
synovial fluid
Articles in the same Issue
- Editorial
- Pneumatic tube delivery systems for patient samples: evidence of quality and quality of evidence
- Reviews
- Translating pharmacogenetics into clinical practice: interleukin (IL)28B and inosine triphosphatase (ITPA) polymophisms in hepatitis C virus (HCV) infection
- New biomarkers for acute renal injury
- Minireview
- Post-mortem biochemistry of vitreous humor and glucose metabolism: an update
- Opinion Paper
- International Osteoporosis Foundation and International Federation of Clinical Chemistry and Laboratory Medicine Position on bone marker standards in osteoporosis
- Guidelines and Recommendations
- IFCC international conventional reference procedure for the measurement of free thyroxine in serum
- Genetics and Molecular Diagnostics
- Improving clinical laboratory efficiency: a time-motion evaluation of the Abbott m2000 RealTime and Roche COBAS AmpliPrep/COBAS TaqMan PCR systems for the simultaneous quantitation of HIV-1 RNA and HCV RNA
- Enhanced frequency of CFTR gene variants in couples who are candidates for assisted reproductive technology treatment
- Customer satisfaction survey to improve the European cystic fibrosis external quality assessment scheme
- General Clinical Chemistry and Laboratory Medicine
- Effect of sample collection, temperature and time of storage on β-galactosidase and total hexosaminidase activities in dried blood collected on filter paper
- Agreement between paired blood gas values in samples transported either by a pneumatic system or by human courier
- Golgi protein 73(GP73), a useful serum marker in liver diseases
- The utility of six over-the-counter (home) pregnancy tests
- N Latex FLC – new monoclonal high-performance assays for the determination of free light chain kappa and lambda
- Alterations of the preoperative coagulation profile in patients with acute appendicitis
- Cerebrospinal fluid findings in infants with pertussis or parapertussis1)
- Calcium pyrophosphate and monosodium urate crystals in synovial fluid as a cause of pseudoeosinophilia
- Cardiovascular Disease
- Dual activity of serum lipoprotein-associated phospholipase A2 yielding positive and inverse associations with cardiometabolic risk
- The value of N-terminal proB-type natriuretic peptide for early identification of myocardial infarction in patients with high-risk non-ST-elevation acute coronary syndromes
- Infectious Diseases
- Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)
- Evaluation of two automated chemiluminescence immunoassays, the LIAISON Treponema Screen and the ARCHITECT Syphilis TP, and the Treponema pallidum particle agglutination test for laboratory diagnosis of syphilis
- Letters to the Editor
- The preanalytical influence of two different mechanical transport systems on laboratory analysis
- Commutability of the ERM-DA470k Reference Material for two assays measuring serum albumin using immunochemical principles
- Performance evaluation of the Vitros®3600 immunodiagnostic system for the determination of free thyroid hormones
- Towards laboratory knowledge, not data, in 70% of clinical decision-making. What “knowledge management” can add to clinical practice?
- How to obtain DNA from injection drug users?
