Home Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)
Article
Licensed
Unlicensed Requires Authentication

Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)

  • Tiparat Potipitak , Warunee Ngrenngarmlert , Chamras Promptmas , Sirinart Chomean and Wanida Ittarat
Published/Copyright: July 18, 2011
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 49 Issue 8

Abstract

Background: Malaria infection with Plasmodium falciparum is an important basic health problem in the tropical and sub-tropical countries. The standard diagnostic method is blood film examination to visualize parasite morphology. However, in cases of low parasitemia or mixed infection with very low cryptic species, microscopy is not sensitive enough. Therefore, molecular techniques have been widely employed.

Methods: A label-free DNA biosensor based on quartz crystal microbalance (QCM) to diagnose and genotype P. falciparum was developed. Avidin-biotin interaction was used to coat the specific biotinylated probe on the gold surface of QCM. The gene encoding merozoite surface protein 2 (msp2) was amplified and the PCR products were then cut with restriction enzyme (MwoI). Enzymatic cutting made the PCR products suitable for QCM development. Hybridization between probe and enzymatic cutting DNA fragments resulted in frequency changes of the QCM.

Results: The newly developed QCM was tested for its diagnosis ability using both malaria laboratory strains and clinical isolates. The biosensor was sensitive at the sub-nanogram level, specific for only P. falciparum detection, no cross-reaction with P. vivax, and stable at room temperature for up to 6 months. Selection of msp2 as a target gene and a geno-typing marker made the QCM potentially useful for falciparum diagnosis simultaneously with genotyping. Potency was tested by genotyping two allelic families of P. falciparum, FC27 and IC1, using malaria laboratory strains, K1 and 3D7, respectively.

Conclusions: The dual function QCM was successfully developed with high sensitivity and specificity, and was cost-effective, stable and field adaptable.

Keywords: biosensor; malaria; merozoite surface protein 2; molecular genotyping; quartz crystal microbalance; recrudescence
Corresponding author: Wanida Ittarat, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon 4 Road, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand Phone: +66-2-4414370-9 ext. 2826, Fax: +66-2-4414380
Received: 2010-09-02
Accepted: 2011-01-21
Published Online: 2011-07-18
Published in Print: 2011-08-01

©2011 by Walter de Gruyter Berlin Boston

Articles in the same Issue

  1. Editorial
  2. Pneumatic tube delivery systems for patient samples: evidence of quality and quality of evidence
  3. Reviews
  4. Translating pharmacogenetics into clinical practice: interleukin (IL)28B and inosine triphosphatase (ITPA) polymophisms in hepatitis C virus (HCV) infection
  5. New biomarkers for acute renal injury
  6. Minireview
  7. Post-mortem biochemistry of vitreous humor and glucose metabolism: an update
  8. Opinion Paper
  9. International Osteoporosis Foundation and International Federation of Clinical Chemistry and Laboratory Medicine Position on bone marker standards in osteoporosis
  10. Guidelines and Recommendations
  11. IFCC international conventional reference procedure for the measurement of free thyroxine in serum
  12. Genetics and Molecular Diagnostics
  13. Improving clinical laboratory efficiency: a time-motion evaluation of the Abbott m2000 RealTime and Roche COBAS AmpliPrep/COBAS TaqMan PCR systems for the simultaneous quantitation of HIV-1 RNA and HCV RNA
  14. Enhanced frequency of CFTR gene variants in couples who are candidates for assisted reproductive technology treatment
  15. Customer satisfaction survey to improve the European cystic fibrosis external quality assessment scheme
  16. General Clinical Chemistry and Laboratory Medicine
  17. Effect of sample collection, temperature and time of storage on β-galactosidase and total hexosaminidase activities in dried blood collected on filter paper
  18. Agreement between paired blood gas values in samples transported either by a pneumatic system or by human courier
  19. Golgi protein 73(GP73), a useful serum marker in liver diseases
  20. The utility of six over-the-counter (home) pregnancy tests
  21. N Latex FLC – new monoclonal high-performance assays for the determination of free light chain kappa and lambda
  22. Alterations of the preoperative coagulation profile in patients with acute appendicitis
  23. Cerebrospinal fluid findings in infants with pertussis or parapertussis1)
  24. Calcium pyrophosphate and monosodium urate crystals in synovial fluid as a cause of pseudoeosinophilia
  25. Cardiovascular Disease
  26. Dual activity of serum lipoprotein-associated phospholipase A2 yielding positive and inverse associations with cardiometabolic risk
  27. The value of N-terminal proB-type natriuretic peptide for early identification of myocardial infarction in patients with high-risk non-ST-elevation acute coronary syndromes
  28. Infectious Diseases
  29. Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)
  30. Evaluation of two automated chemiluminescence immunoassays, the LIAISON Treponema Screen and the ARCHITECT Syphilis TP, and the Treponema pallidum particle agglutination test for laboratory diagnosis of syphilis
  31. Letters to the Editor
  32. The preanalytical influence of two different mechanical transport systems on laboratory analysis
  33. Commutability of the ERM-DA470k Reference Material for two assays measuring serum albumin using immunochemical principles
  34. Performance evaluation of the Vitros®3600 immunodiagnostic system for the determination of free thyroid hormones
  35. Towards laboratory knowledge, not data, in 70% of clinical decision-making. What “knowledge management” can add to clinical practice?
  36. How to obtain DNA from injection drug users?
https://doi.org/10.1515/CCLM.2011.178
Keywords for this article
biosensor; malaria; merozoite surface protein 2; molecular genotyping; quartz crystal microbalance; recrudescence

Articles in the same Issue

  1. Editorial
  2. Pneumatic tube delivery systems for patient samples: evidence of quality and quality of evidence
  3. Reviews
  4. Translating pharmacogenetics into clinical practice: interleukin (IL)28B and inosine triphosphatase (ITPA) polymophisms in hepatitis C virus (HCV) infection
  5. New biomarkers for acute renal injury
  6. Minireview
  7. Post-mortem biochemistry of vitreous humor and glucose metabolism: an update
  8. Opinion Paper
  9. International Osteoporosis Foundation and International Federation of Clinical Chemistry and Laboratory Medicine Position on bone marker standards in osteoporosis
  10. Guidelines and Recommendations
  11. IFCC international conventional reference procedure for the measurement of free thyroxine in serum
  12. Genetics and Molecular Diagnostics
  13. Improving clinical laboratory efficiency: a time-motion evaluation of the Abbott m2000 RealTime and Roche COBAS AmpliPrep/COBAS TaqMan PCR systems for the simultaneous quantitation of HIV-1 RNA and HCV RNA
  14. Enhanced frequency of CFTR gene variants in couples who are candidates for assisted reproductive technology treatment
  15. Customer satisfaction survey to improve the European cystic fibrosis external quality assessment scheme
  16. General Clinical Chemistry and Laboratory Medicine
  17. Effect of sample collection, temperature and time of storage on β-galactosidase and total hexosaminidase activities in dried blood collected on filter paper
  18. Agreement between paired blood gas values in samples transported either by a pneumatic system or by human courier
  19. Golgi protein 73(GP73), a useful serum marker in liver diseases
  20. The utility of six over-the-counter (home) pregnancy tests
  21. N Latex FLC – new monoclonal high-performance assays for the determination of free light chain kappa and lambda
  22. Alterations of the preoperative coagulation profile in patients with acute appendicitis
  23. Cerebrospinal fluid findings in infants with pertussis or parapertussis1)
  24. Calcium pyrophosphate and monosodium urate crystals in synovial fluid as a cause of pseudoeosinophilia
  25. Cardiovascular Disease
  26. Dual activity of serum lipoprotein-associated phospholipase A2 yielding positive and inverse associations with cardiometabolic risk
  27. The value of N-terminal proB-type natriuretic peptide for early identification of myocardial infarction in patients with high-risk non-ST-elevation acute coronary syndromes
  28. Infectious Diseases
  29. Diagnosis and genotyping of Plasmodium falciparum by a DNA biosensor based on quartz crystal microbalance (QCM)
  30. Evaluation of two automated chemiluminescence immunoassays, the LIAISON Treponema Screen and the ARCHITECT Syphilis TP, and the Treponema pallidum particle agglutination test for laboratory diagnosis of syphilis
  31. Letters to the Editor
  32. The preanalytical influence of two different mechanical transport systems on laboratory analysis
  33. Commutability of the ERM-DA470k Reference Material for two assays measuring serum albumin using immunochemical principles
  34. Performance evaluation of the Vitros®3600 immunodiagnostic system for the determination of free thyroid hormones
  35. Towards laboratory knowledge, not data, in 70% of clinical decision-making. What “knowledge management” can add to clinical practice?
  36. How to obtain DNA from injection drug users?
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 28.9.2025 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2011.178/html
Scroll to top button