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Development of a fluorescent microsphere immunoassay for cystatin B (CSTB) in serum of patients with hepatocellular carcinoma
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Na Young Ji
Published/Copyright:
October 20, 2010
Abstract
Background: Serum cystatin B (CSTB) concentrations have been reported to be increased in patients with hepatocellular carcinoma compared to concentrations seen in normal subjects. In this study, we developed a “fluorescent microsphere immunoassay” (FMI) capable of specifically detecting CSTB in serum.
Methods: The FMI used a microparticle conjugated polyclonal antibody to CSTB and biotinylated monoclonal antibody as capture protein and probe protein, respectively. The results were obtained using the Bio-Plex200 system.
Results: The dose-response relationship between CSTB and fluorescent intensity showed linearity in the range 0–1000 pg/mL and 7 pg/mL, sensitivity lower than 11.2 pg/mL. This result revealed that the FMI system was more sensitive than enzyme-linked immunoassay (ELISA). Additionally, the FMI system used smaller sample volumes compared to ELISA.
Conclusions: We measured CSTB with both the FMI and an ELISA procedure and compared the two methods. The CSTB concentrations in serum specimens as measured with the FMI assay system were similar to those measured with ELISA. Thus, the new FMI using the Bio-Plex system may be useful for detection of CSTB in human serum.
Received: 2010-3-18
Accepted: 2010-7-2
Published Online: 2010-10-20
Published in Print: 2011-01-01
©2011 by Walter de Gruyter Berlin New York
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Keywords for this article
cystatin B;
ELISA;
fluorescent microsphere immunoassay;
hepatocellular carcinoma;
serum
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- Publisher's Note
- Editorials
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- Pharmacogenomics of alcohol metabolism: implications for legal testing
- Reviews
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- Opinion Paper
- Harmonization of free thyroid hormone tests: a mission impossible?
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- A high-performance liquid chromatographic method for benznidazole quantitation in plasma of patients with Chagas disease
- Use of serum free light chain analysis and urine protein electrophoresis for detection of monoclonal gammopathies
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