Article
Licensed
Unlicensed
Requires Authentication
Biochemical markers of particle induced osteolysis in C57BL/6 mice
-
Max D. Kauther
Published/Copyright:
August 13, 2010
Abstract
Background: Micro-structural changes associated with ultra high molecular weight polyethylene particle (UHMWPE) induced osteolysis, the most frequent cause of aseptic loosening, have been intensively investigated in the mammalian calvarian model by histomorphometry and micro-computed tomography. However, little is known regarding the serological changes that occur during this process.
Methods: Serological parameters for bone metabolism [calcium, phosphate, osteocalcin (OCN), deoxypyridinoline (DPD)/creatinine, alkaline phosphatase, osteoprotegerin and receptor activator of nuclear factor-κB] were analyzed in this animal model for particle induced osteolysis. Ten C57BL/6 mice were divided at random into sham operated and UHM-WPE implanted groups. Blood and urine samples were collected prior to and at 14 days after surgery.
Results: Implantation of UHMWPE lead to a significant decrease in bone volume (p=0.027). Both groups (sham/UHMWPE) showed a significant increase in calcium (p=0.004/p=0.027) and phosphate (p=0.001/p=0.001), without correlation to particle implantation. Significantly higher concentrations of DPD/creatinine (p=0.034) and OCN (p=0.022) were found after implantation of UHM-WPE. In addition, parameters could not be correlated to particle induced osteolysis.
Conclusions: DPD can be regarded as a valuable parameter for detecting UHMWPE induced osteolysis in the calvarian model. Further studies of serum parameters should focus on the clinical relevance in aseptic prosthetic loosening.
Clin Chem Lab Med 2010;48:1641–6.
Keywords: laboratory parameters; osteolysis; osteolysis model; polyethylene particles; ultra high molecular weight polyethylene particles
Received: 2010-1-9
Accepted: 2010-5-21
Published Online: 2010-08-13
Published in Print: 2010-11-01
©2010 by Walter de Gruyter Berlin New York
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Editorial
- IFCC Scientific Division
- IFCC Documents
- Nucleic acid reference materials (NARMs): definitions and issues
- An appraisal of statistical procedures used in derivation of reference intervals
- An outline for a vocabulary of nominal properties and examinations – basic and general concepts and associated terms
- Review
- Standardizing plasma protein measurements worldwide: a challenging enterprise
- Status Reports
- Standardization activities in the field of thyroid function tests: a status report
- Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: II. Performance of a laboratory network running the HPLC candidate reference measurement procedure and evaluation of a candidate reference material
- Research Articles
- Common reference intervals for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyl transferase (GGT) in serum: results from an IFCC multicenter study
- Development of a candidate secondary reference procedure (immunoassay based measurement procedure of higher metrological order) for cardiac troponin I: I. Antibody characterization and preliminary validation
- Towards the development of a certified reference material for hemoglobin A2
- Short Communication
- First certified reference material for cystatin C in human serum ERM-DA471/IFCC
- General Clinical Chemistry and Laboratory Medicine
- Increased γ-glutamyltransferase and decreased total bilirubin are associated with metabolic syndrome in Korean postmenopausal women
- Effect of homeostasis model assessment computational method on the definition and associations of insulin resistance
- Association between thyroid hormones, lipids and oxidative stress biomarkers in overt hypothyroidism
- Biochemical markers of particle induced osteolysis in C57BL/6 mice
- An on-line solid phase extraction procedure for the routine quantification of urinary methylmalonic acid by liquid chromatography-tandem mass spectrometry
- Cancer Diagnostics
- Increased integrity of circulating cell-free DNA in plasma of patients with acute leukemia
- The relationship between three heat shock protein 70 gene polymorphisms and susceptibility to lung cancer
- Real-time polymerase chain reaction quantification of free DNA in serum of patients with polyps and colorectal cancers
- Letters to the Editor
- Thyroid hormones are stable even during prolonged frozen storage
- Thyroid hormones after long-term cold storage: stable or unstable, that is the question
- Biomarkers for early detection of mesothelioma in asbestos-exposed subjects
- Mean platelet volume and platelet distribution width are useful in the differential diagnosis of aplastic anemia and idiopathic thrombocytopenic purpura
- Kinetics of highly sensitive troponin I and T after eccentric exercise
- Interferences in red blood cell counting in urinalysis using evacuated tubes
- Congress Abstracts
- 3rd Annual Congress of the Austrian Society for Laboratory Medicine and Clinical Chemistry Salzburg, Austria, 27-30 October 2010
Keywords for this article
laboratory parameters;
osteolysis;
osteolysis model;
polyethylene particles;
ultra high molecular weight polyethylene particles
Articles in the same Issue
- Editorial
- IFCC Scientific Division
- IFCC Documents
- Nucleic acid reference materials (NARMs): definitions and issues
- An appraisal of statistical procedures used in derivation of reference intervals
- An outline for a vocabulary of nominal properties and examinations – basic and general concepts and associated terms
- Review
- Standardizing plasma protein measurements worldwide: a challenging enterprise
- Status Reports
- Standardization activities in the field of thyroid function tests: a status report
- Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: II. Performance of a laboratory network running the HPLC candidate reference measurement procedure and evaluation of a candidate reference material
- Research Articles
- Common reference intervals for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyl transferase (GGT) in serum: results from an IFCC multicenter study
- Development of a candidate secondary reference procedure (immunoassay based measurement procedure of higher metrological order) for cardiac troponin I: I. Antibody characterization and preliminary validation
- Towards the development of a certified reference material for hemoglobin A2
- Short Communication
- First certified reference material for cystatin C in human serum ERM-DA471/IFCC
- General Clinical Chemistry and Laboratory Medicine
- Increased γ-glutamyltransferase and decreased total bilirubin are associated with metabolic syndrome in Korean postmenopausal women
- Effect of homeostasis model assessment computational method on the definition and associations of insulin resistance
- Association between thyroid hormones, lipids and oxidative stress biomarkers in overt hypothyroidism
- Biochemical markers of particle induced osteolysis in C57BL/6 mice
- An on-line solid phase extraction procedure for the routine quantification of urinary methylmalonic acid by liquid chromatography-tandem mass spectrometry
- Cancer Diagnostics
- Increased integrity of circulating cell-free DNA in plasma of patients with acute leukemia
- The relationship between three heat shock protein 70 gene polymorphisms and susceptibility to lung cancer
- Real-time polymerase chain reaction quantification of free DNA in serum of patients with polyps and colorectal cancers
- Letters to the Editor
- Thyroid hormones are stable even during prolonged frozen storage
- Thyroid hormones after long-term cold storage: stable or unstable, that is the question
- Biomarkers for early detection of mesothelioma in asbestos-exposed subjects
- Mean platelet volume and platelet distribution width are useful in the differential diagnosis of aplastic anemia and idiopathic thrombocytopenic purpura
- Kinetics of highly sensitive troponin I and T after eccentric exercise
- Interferences in red blood cell counting in urinalysis using evacuated tubes
- Congress Abstracts
- 3rd Annual Congress of the Austrian Society for Laboratory Medicine and Clinical Chemistry Salzburg, Austria, 27-30 October 2010
