Counteracting adriamycin-induced oxidative stress by administration of N-acetyl cysteine and vitamin E
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Periandavan Kalaiselvi
Abstract
Adriamycin (ADR), a cytotoxic antineoplastic drug, is used in the treatment of various solid tumors. However, its efficacy continues to be challenged by significant toxicities including nephrotoxicity. In the present study, the effects of N-acetyl cysteine (NAC) and vitamin E, known antioxidants, were investigated on ADR-induced peroxidative damage in rat kidney. Adult male albino rats of Wistar strain were administered ADR as a single dose (10mg/kg body weight, i.v.). Histopathological studies indicated that ADR-treated kidney sections show focal tubular necrosis and casts. ADR-injected rats showed a significant decline in the activities/levels of enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and glutathione-S-transferase) and non-enzymic antioxidants (thiols, vitamin C and vitamin E) with high malondialdehyde levels. The extent of nephrotoxicity was evident from the increased activities of urinary marker enzymes (alkaline phosphatase, lactate dehydrogenase and γ-glutamyltransferase). Treatment with NAC and vitamin E (50mg/kg b.w., i.p.) 1day prior to ADR administration maintained near normal activities of the enzymes, significantly reduced lipid peroxidation and prevented the necrosis caused by ADR, thereby proving to be an effective thiol replenishing agent and antioxidant.
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©2005 by Walter de Gruyter Berlin New York
Articles in the same Issue
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Articles in the same Issue
- Familial hypercholesterolemia and response to statin therapy according to LDLR genetic background
- Detection of brain injury by fatty acid-binding proteins
- High-throughput scanning of breast tumor surgical specimens for low-level mutations
- Cardiovascular risk-associated allele frequencies for 15 genes in healthy elderly French and Chinese
- Soluble CD30 serum levels before and after treatment with α-interferon in patients with chronic hepatitis C
- Development, validation and evaluation of a homogenous one-step reverse transcriptase-initiated PCR assay with competitive internal control for the detection of hepatitis C virus RNA
- Counteracting adriamycin-induced oxidative stress by administration of N-acetyl cysteine and vitamin E
- Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis
- Thyroglobulin assay 4 weeks after thyroidectomy predicts outcome in low-risk papillary thyroid carcinoma
- Measurement of troponin I 48h after admission as a tool to rule out impaired left ventricular function in patients with a first myocardial infarction
- The efficacy of cystatin C assay in the prediction of glomerular filtration rate. Is it a more reliable marker for renal failure?
- Reference materials (RMs) for analysis of the human factor II (prothrombin) gene G20210A mutation
- Influence of short-term venous stasis on clinical chemistry testing
- Educational intervention together with an on-line quality control program achieve recommended analytical goals for bedside blood glucose monitoring in a 1200-bed university hospital
- The reliability of calculated laboratory results
- Preliminary evaluation of the performance of a new, highly sensitive commercial immunoassay for serum ferritin determination
- Importance of the detection method for intact dimeric human chorionic gonadotropin without interference with the free human chorionic gonadotropin β subunit for pregnancy exclusion before liver transplantation in a woman with cholangiocarcinoma
- Comparison of Liaison N-tact PTH (Diasorin) and N-tact PTH SP IRMA (Diasorin) in hemodialyzed patients
