Advanced oxidation protein products (AOPP) for monitoring oxidative stress in critically ill patients: a simple, fast and inexpensive automated technique
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László Selmeci
Abstract
Oxidative stress is known to be involved in many human pathological processes. Although there are numerous methods available for the assessment of oxidative stress, most of them are still not easily applicable in a routine clinical laboratory due to the complex methodology and/or lack of automation. In research into human oxidative stress, the simplification and automation of techniques represent a key issue from a laboratory point of view at present. In 1996 a novel oxidative stress biomarker, referred to as advanced oxidation protein products (AOPP), was detected in the plasma of chronic uremic patients. Here we describe in detail an automated version of the originally published microplate-based technique that we adapted for a Cobas Mira Plus clinical chemistry analyzer. AOPP reference values were measured in plasma samples from 266 apparently healthy volunteers (university students; 81 male and 185 female subjects) with a mean age of 21.3years (range 18–33). Over a period of 18months we determined AOPP concentrations in more than 300 patients in our department. Our experiences appear to demonstrate that this technique is especially suitable for monitoring oxidative stress in critically ill patients (sepsis, reperfusion injury, heart failure) even at daily intervals, since AOPP exhibited rapid responses in both directions. We believe that the well-established relationship between AOPP response and induced damage makes this simple, fast and inexpensive automated technique applicable in daily routine laboratory practice for assessing and monitoring oxidative stress in critically ill or other patients.
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©2005 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Effect of stress doses of hydrocortisone on S-100B vs. interleukin-8 and polymorphonuclear elastase levels in human septic shock
- Conjugated linoleic acid suppresses the secretion of atherogenic lipoproteins from human HepG2 liver cells
- Determination of debrisoquine and 4-hydroxydebrisoquine by high-performance liquid chromatography: application to the evaluation of CYP2D6 genotype and debrisoquine metabolic ratio relationship
- Assessment of iodine intake in mildly iodine-deficient pregnant women by a new automated kinetic urinary iodine determination method
- Butyrylcholinesterase activity and metabolic syndrome in obese patients
- The non-thyroidal illness syndrome after coronary artery bypass grafting: a 6-month follow-up study
- Advanced oxidation protein products (AOPP) for monitoring oxidative stress in critically ill patients: a simple, fast and inexpensive automated technique
- Quality control in urinary stone analysis: results of 44 ring trials (1980–2001)
- External Quality Assessment in The Netherlands: time to introduce commutable survey specimens. Lessons from the Dutch “Calibration 2000” project
- Evaluation of a high-sensitivity turbidimetric immunoassay for serum C-reactive protein: application to the study of longitudinal changes throughout normal pregnancy
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- Elevated plasma cysteinylglycine levels caused by cilastatin-associated antibiotic treatment
- The number of consultant clinical chemists in the 15-nation European Union
- Biological variation of vascular endothelial growth factor
- Terminology, categories and representation of examinations in laboratory medicine
- Reply to W.G. Wood. Questionable results – who directs the EQAS organisers? Clin Chem Lab Med 2004;42:1073
- The predictive power of serum κ/λ ratios for discrimination between monoclonal gammopathy of undetermined significance and multiple myeloma
- 4th Conference on Hyperhomocysteinemia: Saarbrücken, Germany, April, 14–16, 2005, Saarland University
