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Analysis of inflammatory response in human plasma samples by an automated multicapillary electrophoresis system

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Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Clinical Chemistry and Laboratory Medicine (CCLM) Volume 42 Issue 12

Abstract

A new automated multicapillary zone electrophoresis instrument with a new high-resolution (HR) buffer (Capillarys™ with HR buffer) for analysis of human plasma proteins was evaluated. Albumin, α1-antitrypsin, α1-acid glycoprotein, haptoglobin, fibrinogen, immunoglobulin (Ig)A, IgG and IgM were determined nephelometrically in 200 patient plasma samples. The same samples were then analyzed on the Capillarys™ system (Sebia, Paris, France). The albumin concentration from the nephelometric determination was used for quantification of the individual peaks in the capillary electrophoresis (CE) electropherogram. There was strong linear correlation between the nephelometric and electrophoretic determination of α1-antitrypsin (R2=0.906), α1-acid glycoprotein (R2=0.894) and haptoglobin (R2=0.913). There was also good correlation between the two determinations of γ-globulins (R2=0.883), while the correlation was weaker for fibrinogen (R2=0.377). The Capillarys™ instrument is a reliable system for plasma protein analysis, combining the advantages of full automation, good analytical performance and high throughput. The HR buffer in combination with albumin quantification allows the simultaneous quantification of inflammatory markers in plasma samples without the need for nephelometric determination of these proteins.

Keywords: agarose gel electrophoresis; capillary electrophoresis; human; inflammatory response; nephelometry; plasma
Corresponding author: Anders Larsson, Department of Medical Sciences, University Hospital, 751 85 Uppsala, Sweden Phone: +46-18-6114271, Fax: +46-18-552562,

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Received: 2004-7-30
Accepted: 2004-9-28
Published Online: 2005-6-1
Published in Print: 2004-12-1

©2004 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/CCLM.2004.260
Keywords for this article
agarose gel electrophoresis; capillary electrophoresis; human; inflammatory response; nephelometry; plasma

Articles in the same Issue

  1. Contents
  2. Author Index
  3. Subject Index
  4. Hematopoietic cytokines as tumor markers
  5. The apoB/apoA-I ratio is better than the cholesterol ratios to estimate the balance between plasma proatherogenic and antiatherogenic lipoproteins and to predict coronary risk
  6. Rapid combined genotyping of factor V, prothrombin and methylenetetrahydrofolate reductase single nucleotide polymorphisms using minor groove binding DNA oligonucleotides (MGB probes) and real-time polymerase chain reaction
  7. Methylenetetrahydrofolate reductase gene A222V polymorphism and risk of ischemic stroke
  8. Determination of asymmetric dimethylarginine (ADMA) using a novel ELISA assay
  9. The assessment of bone metabolism in female elite endurance athletes by biochemical bone markers
  10. Declines in serum free and bound choline concentrations in humans after three different types of major surgery
  11. Analysis of inflammatory response in human plasma samples by an automated multicapillary electrophoresis system
  12. Multicenter evaluation of the commutability of a potential reference material for harmonization of enzyme activities
  13. HDL and clinical and biochemical correlates in Italian non-smoker women
  14. Hyperhomocysteinaemia is associated with uveitis but not with deep venous thrombosis in Behçet's disease
  15. Production of gender-specific morning salivary cortisol reference intervals using internationally accepted procedures
  16. Concentrations of N-terminal pro-brain natriuretic peptide and troponin T in plasma of 75-year-old apparently healthy persons
  17. External Quality Assessment for biochemical markers of myocardial damage: an Italian experience
  18. Further evaluation of the immunonephelometric assay of soluble transferrin receptor and assessment of pediatric values in southern Italy
  19. Exercise raises serum heat-shock protein 70 (Hsp70) levels
  20. Hepatitis C-associated complement cold activation
  21. The relationship between cryoglobulins and HCV-RNA in the serum of patients infected with different HCV genotypes
  22. Artificial neural network-based algorithm for the evaluation of serum protein capillary electrophoresis
  23. State-of-the-art systematic error for therapeutic drug monitoring
  24. Peculiarities and problems with the EXCEL® F-test
  25. Questionable results – should EQA results be accepted as submitted or should ‘obvious mistakes’ be corrected?. Reply to Wood WG. Questionable results – who directs the EQAS organisers? CCLM 2004;42(9):1073
  26. Erratum
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