Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease
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Peter Bugert
Abstract
P-selectin and its ligand, PSGL-1, are cell adhesion molecules that facilitate interaction of platelets, leukocytes and endothelial cells. Polymorphisms of these genes have been reported to be associated with coronary heart disease (CHD). In the present study, we characterized the entire coding regions of P-selectin and PSGL-1 genes in CHD patients and healthy controls. The 17 exons of the P-selectin gene and exon 2 of the PSGL-1 gene were screened for single nucleotide polymorphisms (SNPs) by exon re-sequencing in 88 CHD patients and 96 controls. For rapid genotyping of the SNPs we developed PCR techniques with sequence-specific primers (PCR-SSP). By using PCR-SSPs we genotyped 261 CHD patients and 214 controls for 5 SNPs in P-selectin and 2 SNPs in PSGL-1. In addition to the already described SNPs in P-selectin (S290N, N562D, V599L and T715P), we identified a novel SNP in exon 5 (V168M). The P-selectin 715P allele was more frequent among CHD patients with hypercholesterolemia compared to patients with normal cholesterol levels. A SNP (M62I) in the PSGL-1 gene was found close to the P-selectin binding site and the 62I allele revealed a higher prevalence in the control group indicating a protective effect of the mutation. The molecular characterization of P-selectin and PSGL-1 in a case-control study including CHD patients and healthy controls revealed evidence for association of the genes with development of the disease. However, the functional role of the gene variants should be elucidated by further experimental data.
References
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© Walter de Gruyter
Articles in the same Issue
- ARH missense polymorphisms and plasma cholesterol levels
- Genetic and immunological analyses of patients with increased serum butyrylcholinesterase activity and its C5 variant form
- Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease
- Odd-numbered long-chain fatty acids in erythrocyte phospholipids as long-term follow-up parameter in propionic acidemia
- Simplified method for the diameter sizing of serum low-density lipoprotein using polyacrylamide gradient gel electrophoresis
- In vitro inhibition of fibrinolysis by apolipoprotein(a) and lipoprotein(a) is size- and concentration-dependent
- Comparison of cardiac troponin T and I in healthy men and in aortic valve replacement
- Evaluation of a shorter methionine loading test
- Homocysteine, vitamin B12 and folate in vascular dementia and in Alzheimer disease
- The clinical impact of screening for gestational diabetes
- Comparison between creatinine and pregnanediol adjustments in the retrospective analysis of urinary hormone profiles during the human menstrual cycle
- The measurement of complexed prostate-specific antigen has a better performance than total prostate-specific antigen
- Effect of prolonged physical exercise on urinary proANP1-30 and proANP31-67
- Diagnostic efficiency of different amphetamine screening tests – the search for an optimal cutoff
- Evaluation of a new lithium colorimetric assay performed on the Dade Behring Dimension® X-pand™ system
- Reference interval of serum insulin concentrations
- Significant decimals and rounding
- Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers? A comment from the point of view of the ECAT Foundation
- International Swiss MedLab 2004 and 8th Alps Adria Congress / Laboratory Medicine: From Atomic absorption to Zeta-Globin Lucerne, Switzerland, October 5–9, 2004
Articles in the same Issue
- ARH missense polymorphisms and plasma cholesterol levels
- Genetic and immunological analyses of patients with increased serum butyrylcholinesterase activity and its C5 variant form
- Polymorphisms in the P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (PSGL-1) genes and coronary heart disease
- Odd-numbered long-chain fatty acids in erythrocyte phospholipids as long-term follow-up parameter in propionic acidemia
- Simplified method for the diameter sizing of serum low-density lipoprotein using polyacrylamide gradient gel electrophoresis
- In vitro inhibition of fibrinolysis by apolipoprotein(a) and lipoprotein(a) is size- and concentration-dependent
- Comparison of cardiac troponin T and I in healthy men and in aortic valve replacement
- Evaluation of a shorter methionine loading test
- Homocysteine, vitamin B12 and folate in vascular dementia and in Alzheimer disease
- The clinical impact of screening for gestational diabetes
- Comparison between creatinine and pregnanediol adjustments in the retrospective analysis of urinary hormone profiles during the human menstrual cycle
- The measurement of complexed prostate-specific antigen has a better performance than total prostate-specific antigen
- Effect of prolonged physical exercise on urinary proANP1-30 and proANP31-67
- Diagnostic efficiency of different amphetamine screening tests – the search for an optimal cutoff
- Evaluation of a new lithium colorimetric assay performed on the Dade Behring Dimension® X-pand™ system
- Reference interval of serum insulin concentrations
- Significant decimals and rounding
- Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers?
- Wood WG. Questionable results – who directs the EQAS organisers? A comment from the point of view of the ECAT Foundation
- International Swiss MedLab 2004 and 8th Alps Adria Congress / Laboratory Medicine: From Atomic absorption to Zeta-Globin Lucerne, Switzerland, October 5–9, 2004