Determination of Nitrite Plus Nitrate and Malondialdehyde in Human Plasma: Analytical Performance and the Effect of Smoking and Exercise
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Cristina Vassalle
Abstract
The aim of this study was to evaluate the analytical performance and clinical usefulness of spectrophotometric assays for the measurement of the plasma levels of nitrite plus nitrate (NOx), and malondialdehyde (MDA), as an index of nitric oxide release and lipid peroxidation, respectively. We studied 30 healthy sedentary volunteers, 12 endurance athletes and 12 regular heavy smokers. The lower limit of quantification for plasma NOx concentration was 1 μmol/l, and linearity was observed from 1 to 40 μmol/l of NOx concentration. Variation in replicate samples within or between days was always below 5%. NOx levels were significantly higher in athletes compared to both control subjects and smokers (p<0.05 and p<0.001, respectively), as well as in healthy subjects compared to smokers (p<0.05). The analytical limit of quantification for plasma MDA concentration was 0.03 μmol/l, and linearity was observed from 0.03 to 20 μmol/l of MDA concentration. Variation in replicate samples within or between runs was <5%. Mean MDA concentration was significantly higher in smokers compared to control subjects and athletes (p<0.001). A significant inverse relationship (p<0.001) was observed when comparing NOx with MDA (r= −0.49) or LDL levels (r= −0.30) in the total population. The assays evaluated in this study proved to be sensitive, specific and practicable, and therefore suitable for routine application in clinical chemistry laboratories and/or physiopathological studies involving human blood
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- SIBioC 2002 - 34th National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology, Rimini, Italy, 17-20 September 2002
- Santorini Biologie Prospective Conference 2002 From Genetic Variations to Risk Prediction and Pharmacogenomics Santorini, Greece, 25-28 September 2002
- CCLM Special Issue on Diabetes Mellitus - Call for Papers
- Pathophysiological and Clinical Relevance of Circulating Levels of Cardiac Natriuretic Hormones: Are They Merely Markers of Cardiac Disease?
- Comparative Measurement of N-Terminal Pro-Brain Natriuretic Peptide and Brain Natriuretic Peptide in Ambulatory Patients with Heart Failure
- Tumor Necrosis Factor-α-Induced Accumulation of Tumor Suppressor Protein p53 and Cyclin-dependent Protein Kinase Inhibitory Protein p21 Is Inhibited by Insulin in ME-180S Cells
- Inflammatory Response to Coronary Stent Implantation in Patients with Unstable Angina
- Immature Granulocyte Count after Liver Transplantation
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- Defining the Period of Recovery of the Glucose Concentration after Its Local Perturbation by the Implantation of a Miniature Sensor
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- Quantitative Determination of Faecal Fatty Acids and Triglycerides by Fourier Transform Infrared Analysis with a Sodium Chloride Transmission Flow Cell
- Rapid Identification of Atypical Variant of Plasma Butyrylcholinesterase by PCR
- Determination of Nitrite Plus Nitrate and Malondialdehyde in Human Plasma: Analytical Performance and the Effect of Smoking and Exercise
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- A Novel Explanation for Low Alanine Aminotransferase Activity in Human Serum Caused by IgG Inhibitor
- Serum Total Antioxidant Status Is Higher in Postmenopausal Women and after Estrogen Replacement Therapy
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