Home Medicine Sensitive Chemiluminescence Immunoassay for the Determination of Rat Serum α1-Acid Glycoprotein
Article
Licensed
Unlicensed Requires Authentication

Sensitive Chemiluminescence Immunoassay for the Determination of Rat Serum α1-Acid Glycoprotein

  • Jochen Metzger , Manfred Blobner and Peter B. Luppa
Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 39 Issue 6

Abstract

We present the establishment of a sensitive immunoassay for the determination of α1-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.

:
Published Online: 2005-06-01
Published in Print: 2001-06-27

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Clinical Laboratoy Science in the Balkan Region
  2. Peroxisome Proliferators: Their Biological and Toxicological Effects
  3. Medical Biochemistry in Macedonia: a Profession for Physicians and Natural Scientists
  4. Stress Tolerance Test and SDS-PAGE for the Analysis of Urinary Proteins in Children and Youths
  5. Atherosclerosis Risk Factors Related to HemodialysisDuration and Erythropoietin Therapy
  6. Maternal Serum Screening for Downs Syndrome, Open Neural Tube Defects and Trisomy 18
  7. The Effect of Escherichia Coli-Derived Lipopolysaccharides on Plasma Levels of Malondialdehyde and 3-Nitrotyrosine
  8. Isolation of Fetal Cells from the Maternal Circu-lation:Prospects for the Non-Invasive Prenatal Diagnosis
  9. Melting Temperature Analysis as Quantitative Method for Detection of Point Mutations
  10. A Novel Missense Mutation C127R (FH Zagreb) in the LDL-Receptor Gene
  11. Plasma Endothelin-1 and Big Endothelin-1 Levels in Superior and Inferior Vena Cava during ProtractedAntiorthostatic Hypokinetic/Hypodynamia in Rats
  12. Sensitive Chemiluminescence Immunoassay for the Determination of Rat Serum α1-Acid Glycoprotein
  13. Increased Serum and Salivary Immunoglobulins against Candida albicans in HIV-Infected Patients with Oral Candidiasis
  14. Elevated Carbohydrate Antigen 19-9 (CA 19-9) in Patients with Echinococcus Infection
  15. Interpretation of Sequential Measurements of Cancer Antigen 125 (CA 125), Carcinoembryonic Antigen (CEA) and Tissue Polypeptide Antigen (TPA) Based on Analytical Imprecision and Biological Variation in the Monitoring of Ovarian Cancer
  16. Inter-Laboratory Evaluation of the COBAS INTEGRA® 400 Analytical System
  17. Educational Letter
  18. Protein G ELISA for the Detection of Circulating Immune Complexes. An Alternative to the Solid-Phase Radioimmunoassay for the Monoclonal Rheumatoid Factor
  19. Book Review: AZ of Medical Writing by Tim Albert
  20. Cecil Essentials of Medicine by T.E. Andreoli, C.C.J. Carpenter, R.C. Griggs and J. Loscalzo, editors
https://doi.org/10.1515/CCLM.2001.086

Articles in the same Issue

  1. Clinical Laboratoy Science in the Balkan Region
  2. Peroxisome Proliferators: Their Biological and Toxicological Effects
  3. Medical Biochemistry in Macedonia: a Profession for Physicians and Natural Scientists
  4. Stress Tolerance Test and SDS-PAGE for the Analysis of Urinary Proteins in Children and Youths
  5. Atherosclerosis Risk Factors Related to HemodialysisDuration and Erythropoietin Therapy
  6. Maternal Serum Screening for Downs Syndrome, Open Neural Tube Defects and Trisomy 18
  7. The Effect of Escherichia Coli-Derived Lipopolysaccharides on Plasma Levels of Malondialdehyde and 3-Nitrotyrosine
  8. Isolation of Fetal Cells from the Maternal Circu-lation:Prospects for the Non-Invasive Prenatal Diagnosis
  9. Melting Temperature Analysis as Quantitative Method for Detection of Point Mutations
  10. A Novel Missense Mutation C127R (FH Zagreb) in the LDL-Receptor Gene
  11. Plasma Endothelin-1 and Big Endothelin-1 Levels in Superior and Inferior Vena Cava during ProtractedAntiorthostatic Hypokinetic/Hypodynamia in Rats
  12. Sensitive Chemiluminescence Immunoassay for the Determination of Rat Serum α1-Acid Glycoprotein
  13. Increased Serum and Salivary Immunoglobulins against Candida albicans in HIV-Infected Patients with Oral Candidiasis
  14. Elevated Carbohydrate Antigen 19-9 (CA 19-9) in Patients with Echinococcus Infection
  15. Interpretation of Sequential Measurements of Cancer Antigen 125 (CA 125), Carcinoembryonic Antigen (CEA) and Tissue Polypeptide Antigen (TPA) Based on Analytical Imprecision and Biological Variation in the Monitoring of Ovarian Cancer
  16. Inter-Laboratory Evaluation of the COBAS INTEGRA® 400 Analytical System
  17. Educational Letter
  18. Protein G ELISA for the Detection of Circulating Immune Complexes. An Alternative to the Solid-Phase Radioimmunoassay for the Monoclonal Rheumatoid Factor
  19. Book Review: AZ of Medical Writing by Tim Albert
  20. Cecil Essentials of Medicine by T.E. Andreoli, C.C.J. Carpenter, R.C. Griggs and J. Loscalzo, editors
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2026 De Gruyter Brill
Downloaded on 21.2.2026 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2001.086/pdf
Scroll to top button