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Unravelling Genetic Data by Arrayed Primer Extension
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Neeme Tõnisson
Published/Copyright:
June 1, 2005
Abstract
We have developed a method for arrayed primer extension (APEX) on an oligonucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and changes in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphism (SNP) chip for genotyping were used as a model system. Chip surface chemistry, template preparation and APEX reaction conditions were optimised and the assay is ready to be implemented in variety of DNA analysis from SNP testing to DNA resequencing.
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Published Online: 2005-06-01
Published in Print: 2000-02-05
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- IFCC WorldLab '99
- Markers of Endothelial Dysfunction
- Standardization and Quality Control of PCR Analyses
- Histochemical and Immunocytochemical Approaches to the Study of Oxidative Stress
- Sniffing out the Truth: Clinical Diagnosis Using the Electronic Nose
- Oncogenes and Thyroid Cancer
- Paraneoplastic Syndromes of the Nervous System
- Genetic Modifying Factors in β-Thalassemia
- The Genetics of Multiple Sclerosis
- Near-Infrared Reflectance Spectroscopy for Noninvasive Monitoring of Metabolites
- Ribozyme Gene Therapy for Autosomal Dominant Retinal Disease
- Oxidized Lipoproteins and Endothelium
- Experiences with External Quality Assessment (EQA) in Molecular Diagnostics in Clinical Laboratories in Germany
- Unravelling Genetic Data by Arrayed Primer Extension
- Evaluation of RNA Isolation Methods and Reference Genes for RT-PCR Analyses of Rare Target RNA
- IFCC WorldLab Daily News
