Automation of Urine Sediment Examination: a Comparison of the Sysmex UF-100 Automated Flow Cytometer with Routine Manual Diagnosis (Microscopy, Test Strips, and Bacterial Culture)
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Kurt Hannemann-Pohl
Abstract
Urine specimens from 438 patients were examined with the UF-100 flow cytometer (Sysmex TOA Medical Electronics (Europe) GmbH, Hamburg, Germany) and by manual microscopy and test strips. One hundred and forty-two of these were also examined bacteriologically. The measurements with the UF-100 were performed on native urine without prior centrifugation. Intraassay imprecision, CV of 1.3 % (547/μl) to 8.5 % (24/μl) for erythrocytes and CV of 2.4 % ( 218/μl) to 5.6 % (10/μl) for leukocytes, are similar to those usual in clinical chemistry, and are very much better than those seen in manual microscopy of sediment. In routine use, overloading the flow cytometer by an excessive concentration of particles was observed in 9% of specimens. Such specimens should be checked visually. The UF-100 is distinctly more sensitive than manual microscopy for determining leukocytes, erythrocytes, epithelial cells and bacteria. Reference ranges were estimated from the results obtained. These enabled the UF-100 to replace routine manual diagnostic methods. Although the sensitivity is improved over manual microscopy of sediment, it is always necessary to perform parallel test strip examination when determining erythrocytes in order to detect haemolysis.
In our opinion the Sysmex UF-100 is a suitable replacement for manual microscopy of urine sediment. In addition it offers an opportunity to improve standardization of basic urinalysis.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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- Automation of Urine Sediment Examination: a Comparison of the Sysmex UF-100 Automated Flow Cytometer with Routine Manual Diagnosis (Microscopy, Test Strips, and Bacterial Culture)
- Characterization of the Isoenzyme Profile of β-N-Acetylhexosaminidase in the Urine of Newborns
- Clinical Laboratory Diagnostics. Thomas Lothar, editor
- Structure-based Ligand Design. Klaus Gubernator and Hans-J. Böhm, editors (Methods and Principles in Medicinal Chemistry, vol. 6.)
Articles in the same Issue
- Development of Molecular Genetics
- The Changing Face of Clinical Laboratories
- Analysis of the Gly40Ser Polymorphism in the Glucagon Receptor Gene in a German Non-Insulin-Dependent Diabetes Mellitus Population
- A Relationship between K-ras Gene Mutations and Some Clinical and Histologic Variables in Patients with Primary Colorectal Carcinoma
- Particle Counting Immunoassay for Urinary Cotinine. Comparison with Chromatography, Enzyme-linked Immunoassay and Fluorescence Polarization Immunoassay
- Identification of IgG-specific Oligoclonal Banding in Serum and Cerebrospinal Fluid by Isoelectric Focusing: Description of a Simplified Method for the Diagnosis of Neurological Disorders
- Low Bone Mineral Density after Total Gastrectomy in Males: a Preliminary Report Emphasizing the Possible Significance of Urinary Net Acid Excretion, Serum Gastrin and Phosphorus
- Six Methods for the Determination of C-Peptide Evaluated
- Automation of Urine Sediment Examination: a Comparison of the Sysmex UF-100 Automated Flow Cytometer with Routine Manual Diagnosis (Microscopy, Test Strips, and Bacterial Culture)
- Characterization of the Isoenzyme Profile of β-N-Acetylhexosaminidase in the Urine of Newborns
- Clinical Laboratory Diagnostics. Thomas Lothar, editor
- Structure-based Ligand Design. Klaus Gubernator and Hans-J. Böhm, editors (Methods and Principles in Medicinal Chemistry, vol. 6.)
