Particle Counting Immunoassay for Urinary Cotinine. Comparison with Chromatography, Enzyme-linked Immunoassay and Fluorescence Polarization Immunoassay
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Laurence M. Galanti
Abstract
Urinary cotinine was measured according to its inhibitory activity on the agglutination of cotinine-coated latex particles by anti-cotinine antibodies, the agglutination being measured by optical counting of the remaining non-agglutinated particles (particle counting, PaC). The detection limit was 0.03 μg/ml and the practical range extended from 0.03 to 3.9 μg/ml. The correlation results of 320 urine samples with those of high pressure liquid chromatography, enzyme-linked (Coti-Tracq EIA, Serex Inc., Maywood, NJ, USA), and fluorescence polarization immunoassay (TDX instrument, Abbott, Abbott Park, IL, USA) were r = 0.90, r = 0.69, r = 0.87, respectively, whereas the correlation coefficients between the assays other than particle counting ranged from 0.62 to 0.88. PaC does not require any separation step and can thus be easily automated.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Development of Molecular Genetics
- The Changing Face of Clinical Laboratories
- Analysis of the Gly40Ser Polymorphism in the Glucagon Receptor Gene in a German Non-Insulin-Dependent Diabetes Mellitus Population
- A Relationship between K-ras Gene Mutations and Some Clinical and Histologic Variables in Patients with Primary Colorectal Carcinoma
- Particle Counting Immunoassay for Urinary Cotinine. Comparison with Chromatography, Enzyme-linked Immunoassay and Fluorescence Polarization Immunoassay
- Identification of IgG-specific Oligoclonal Banding in Serum and Cerebrospinal Fluid by Isoelectric Focusing: Description of a Simplified Method for the Diagnosis of Neurological Disorders
- Low Bone Mineral Density after Total Gastrectomy in Males: a Preliminary Report Emphasizing the Possible Significance of Urinary Net Acid Excretion, Serum Gastrin and Phosphorus
- Six Methods for the Determination of C-Peptide Evaluated
- Automation of Urine Sediment Examination: a Comparison of the Sysmex UF-100 Automated Flow Cytometer with Routine Manual Diagnosis (Microscopy, Test Strips, and Bacterial Culture)
- Characterization of the Isoenzyme Profile of β-N-Acetylhexosaminidase in the Urine of Newborns
- Clinical Laboratory Diagnostics. Thomas Lothar, editor
- Structure-based Ligand Design. Klaus Gubernator and Hans-J. Böhm, editors (Methods and Principles in Medicinal Chemistry, vol. 6.)
