A Relationship between K-ras Gene Mutations and Some Clinical and Histologic Variables in Patients with Primary Colorectal Carcinoma
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Martin Beránek
Abstract
Mutations in the Kirsten ras 2 (K-ras) gene were described as early events in the process of colorectal carcinogenesis. The aim of this study was to find a possible relationship between the presence of K-ras mutation in samples of primary colorectal carcinomas and the clinico-pathological data of the investigated patients. Mutation in codon 12 of the K-ras gene was determined in 18 of 53 colorectal carcinomas (34 %) in our group of patients. The presence of K-ras gene mutations was not related to gender, age of subject at diagnosis, staging or cancer location (p > 0.05). Sixteen of the 42 (38 %) moderately differentiated carcinomas, and two of the eight (25 %) well differentiated carcinomas contained K-ras mutation in codon 12, but none of the three poorly differentiated carcinomas contained the mutation. Moderately differentiated tumours contained an aspartate code GAT (in eight cases), a valine code GTT (in six cases), an alanine code GCT (in one case) and a serine code AGT (in one case) in codon 12. Well differentiated tumours contained only the valine code GTT (two cases).
Our results show that the frequency of mutations in the K-ras gene in carcinomas in Central Europe is not different from the frequencies found in other parts of the world. The homogeneous incidence of K-ras mutation does not seem to be related to ethnic factors, dietary habits, or the composition of the diet.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Development of Molecular Genetics
- The Changing Face of Clinical Laboratories
- Analysis of the Gly40Ser Polymorphism in the Glucagon Receptor Gene in a German Non-Insulin-Dependent Diabetes Mellitus Population
- A Relationship between K-ras Gene Mutations and Some Clinical and Histologic Variables in Patients with Primary Colorectal Carcinoma
- Particle Counting Immunoassay for Urinary Cotinine. Comparison with Chromatography, Enzyme-linked Immunoassay and Fluorescence Polarization Immunoassay
- Identification of IgG-specific Oligoclonal Banding in Serum and Cerebrospinal Fluid by Isoelectric Focusing: Description of a Simplified Method for the Diagnosis of Neurological Disorders
- Low Bone Mineral Density after Total Gastrectomy in Males: a Preliminary Report Emphasizing the Possible Significance of Urinary Net Acid Excretion, Serum Gastrin and Phosphorus
- Six Methods for the Determination of C-Peptide Evaluated
- Automation of Urine Sediment Examination: a Comparison of the Sysmex UF-100 Automated Flow Cytometer with Routine Manual Diagnosis (Microscopy, Test Strips, and Bacterial Culture)
- Characterization of the Isoenzyme Profile of β-N-Acetylhexosaminidase in the Urine of Newborns
- Clinical Laboratory Diagnostics. Thomas Lothar, editor
- Structure-based Ligand Design. Klaus Gubernator and Hans-J. Böhm, editors (Methods and Principles in Medicinal Chemistry, vol. 6.)
