Effect of the Hemoglobin-Based Oxygen Carrier HBOC-201 on Laboratory Instrumentation: Cobas Integra, Chiron Blood Gas Analyzer 840, Sysmex™SE-9000 and BCT
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Albert Wolthuis
Abstract
As part of a clinical trial, we evaluated the effects of the hemoglobin-based oxygen-carrier (HBOC) HBOC-201 (an ultrapurified, stroma-free bovine hemoglobin product, Biopure®, Cambridge, MA, USA) on our routine clinical chemistry analyzer (Cobas Integra, F. Hoffmann-La Roche Ltd, Basel, Switzerland ), blood gas analyzer (Chiron 840, Chiron Diagnostics Corporation, East Walpole, MA, USA), routine hemocytometry analyzer (Sysmex™ SE-9000, TOA Medical Electronics Co Ltd., Kobe, Japan), hemostasis analyzer (BCT, Dade-Behring, Marburg, Germany) and bloodbanking system (Dia-Med-ID Micro Typing System, DiaMed AG, Cressier, Switzerland). The maximum tested concentration of HBOC-201 was 65 g/l. Of the 27 routine clinical chemistry tests challenged with HBOC-201, bilirubin-direct, creatine kinase MB-fraction (CK-MB), creatine kinase (CK), γ-glutamyltransferase (GGT), magnesium and uric acid were influenced by even low concentrations of HBOC-201. These tests were excluded from use on the plasma of patients treated with HBOC-201. Since the non-availability of the cardiac marker CK-MB may lead to problems in acute situations, we introduced the qualitative Trop T-test (Boehringer Mannheim), which was not influenced. The applicability of another nine tests was limited by the concentration of the HBOC-201 in the patients' plasma. No interference of HBOC-201 in routine hemocytometry, hemostasis-analysis and red-blood cell agglutination detection (blood-bank tests) was observed. Although immediate patient-care was not compromised, routine use of hemoglobin-based oxygen carriers will have a strong impact on logistical management. The development of robust laboratory tests free from the interference of the pigmented oxygen carriers should therefore precede its introduction into routine transfusion medicine.
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
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- Structure, Function and Clinical Significance of Transferrin Receptors
- Cardiac Troponin I and Troponin T: Recent Players in the Field of Myocardial Markers
- Determination of Free Apolipoprotein(a) in Serum by Immunoassay and Its Significance for Risk Assessment in Patients with Coronary Artery Disease
- Fecal Tumor Necrosis Factor alpha, Eosinophil Cationic Protein and IgE Levels in Infants with Cows Milk Allergy and Gastrointestinal Manifestations
- Detection of GB Virus C/Hepatitis G Virus RNA by Reverse-Transcription Polymerase Chain Reaction
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- Effect of the Hemoglobin-Based Oxygen Carrier HBOC-201 on Laboratory Instrumentation: Cobas Integra, Chiron Blood Gas Analyzer 840, Sysmex™SE-9000 and BCT
- Current System of Undergraduate and Postgraduate Education in Clinical Chemistry in Croatia
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- Molecular Biology or Steroid and Nuclear Hormone Receptors by Leonard P. Freedman, editor
