Article
Licensed
Unlicensed
Requires Authentication
Mutation C677T of Methylenetetrahydrofolate Reductase Gene Is Not Associated with Coronary Artery Disease, but Possibly with Albuminuria, in Type 2 Diabetic Patients
-
Valtteri Wirta
Published/Copyright:
June 1, 2005
Abstract
The missense mutation in the 677th nucleotide (C677T) of methylenetetrahydrofolate reductase gene causes substitution of valine (V) for alanine (A) resulting in three genotypes VV, VA and AA. The VV genotype causes hyperhomocysteinemia and may be a risk factor for coronary artery disease. We determined genotypes by polymerase chain reaction and subsequent restriction fragment length analysis and compared them in 84 patients with type 2 diabetes and in 115 non-diabetic subjects with and without coronary disease. Fractional urinary excretion rate of albumin was assessed by nephelometry. The VV, VA, and AA frequencies in the diabetic and in the control groups were 0.095, 0.357, 0.548 and 0.061, 0.417, 0.522, respectively (p = NS, diabetic vs. controls, χ2 test). Genotype frequencies did not differ in either diabetic or control subjects between those with or those without coronary disease (χ2 test). The fractional urinary excretion rate of albumin (mean ±SD) in diabetic patients with the VV genotype i.e. 1.59 ± 0.71 was lower (Kruskall-Wallis test p = 0.002) than in the other genotypes i.e. VA 5.98 ± 9.75 and AA 3.75 ± 4.77, respectively (post-hoc Mann-Whitney test VV vs. VA p = 0.005 and VV vs. AA p = 0.054, respectively). We found that in patients with type 2 diabetes the methylenetetrahydrofolate reductase VV genotype was associated with a low urinary albumin excretion but not with coronary artery disease or diabetes per se.
:
Published Online: 2005-06-01
Published in Print: 1998-08-01
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Human Genomics: The Basis of the Medicine of Tomorrow. Recent Progress in Molecular Biology Technology. First IFCC-Roche Conference – Singapore, 15–18 March 1998
- Does Clinical Chemistry Have a Future?
- The Human Genome Project: From Mapping to Sequencing
- The Human Genome Project and the Role of Genetics in Health Care
- Mutation Detection and Mutation Databases
- Population Genomics: Laying the Groundwork for Genetic Disease Modeling and Targeting
- Oligonucleotide-Based Gene Therapy for Cardiovascular Disease
- Signal Transduction Pathways and Modulation of Gene Activity
- Do Overexpressed Oncoproteins Cause Malignant Growth of Cancer Cells? – Studied by Antisense Oligonucleotides
- Ethical and Legal Issues in the Procurement, Storage and Use of DNA
- DNA Extraction and Stability for Epidemiological Studies
- Simple and Broadly Applicable Sample Preparation by Use of Magnetic Glass Particles
- A Multilocus Genotyping Assay for Cardiovascular Disease
- Optimized Non-Radioactive Protein Truncation Test for Mutation Analysis of the Adenomatous Polyposis Coli (APC) Gene
- Monitoring of Inhibitors of Enzymatic Amplification in Polymerase Chain Reaction and Evaluation of Efficacy of RNA Extraction for the Detection of Hepatitis C Virus Using the Internal Control
- Quantification of Relative Expression of Genes with Homologous Sequences Using Fluorescence-Based Single-Strand Conformation Polymorphism Analysis – Application to Lactate Dehydrogenase and Cyclooxygenase Isozymes
- Automation of Polymerase Chain Reaction-Based Systems for Detection of Hepatitis C Virus RNA
- A Simple PCR with Different 3′ Ends of the Third Primer for Detection of Defined Point Mutations: HCV Genotyping as an Example
- Two Quantitative Methods for Imaging Samples in Molecular Biology
- Restriction Digest PCR (RD-PCR) for the Analysis of Gene Mutations. Application to Ki-ras
- Direct Detection of Mycobacterium tuberculosis in Respiratory Specimens Using an Automated DNA Amplification Assay and a Single Tube Nested Polymerase Chain Reaction (PCR)
- Quantitative Detection of Hepatitis B Virus DNA with a New PCR Assay
- Molecular Diagnostics for Cardiovascular Disease
- Association of Apolipoprotein E Polymorphism and Concentration with Serum Lipids and Apo-lipoprotein Level in the Chinese fromShanghai
- Angiotensin Converting Enzyme Gene Polymorphism in Coronary Artery Disease: The Indian Scenario
- Mutation C677T of Methylenetetrahydrofolate Reductase Gene Is Not Associated with Coronary Artery Disease, but Possibly with Albuminuria, in Type 2 Diabetic Patients
- Cholesteryl Ester Transfer Protein Mutations, Protein Activity and HDL-Cholesterol Concentration
- Molecular Biology of Alcohol Dependence, a Complex Polygenic Disorder
- Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR
- The Impact of Molecular Diagnosis on Familial Colorectal Cancer
- Screening for Tumour Suppressor p16(CDKN2A) Germline Mutations in Israeli Melanoma Families
- CGG Repeat Interruptions in the FMR1 Gene in Patients with Infantile Autism
- Detection of CYP2D6*3 and 2D6*4 Allelic Variants by PCR-Restriction Fragment Length Polymorphism
- Genetic Basis of Congenital Hypothyroidism: Abnormalities in the TSHβ Gene, the PIT1 Gene, and the NIS Gene
- Follitropin (FSH) Deficiency in an Infertile Male due to FSHβ Gene Mutation. A Syndrome of Normal Puberty and Virilization but Under-developed Testicles with Azoospermia, Low FSH but High Lutropin and Normal Serum Testosterone Concentrations
- Molecular Analysis and Electromyoneurographic Abnormalities in Croatian Children with Proximal Spinal Muscular Atrophies
Articles in the same Issue
- Human Genomics: The Basis of the Medicine of Tomorrow. Recent Progress in Molecular Biology Technology. First IFCC-Roche Conference – Singapore, 15–18 March 1998
- Does Clinical Chemistry Have a Future?
- The Human Genome Project: From Mapping to Sequencing
- The Human Genome Project and the Role of Genetics in Health Care
- Mutation Detection and Mutation Databases
- Population Genomics: Laying the Groundwork for Genetic Disease Modeling and Targeting
- Oligonucleotide-Based Gene Therapy for Cardiovascular Disease
- Signal Transduction Pathways and Modulation of Gene Activity
- Do Overexpressed Oncoproteins Cause Malignant Growth of Cancer Cells? – Studied by Antisense Oligonucleotides
- Ethical and Legal Issues in the Procurement, Storage and Use of DNA
- DNA Extraction and Stability for Epidemiological Studies
- Simple and Broadly Applicable Sample Preparation by Use of Magnetic Glass Particles
- A Multilocus Genotyping Assay for Cardiovascular Disease
- Optimized Non-Radioactive Protein Truncation Test for Mutation Analysis of the Adenomatous Polyposis Coli (APC) Gene
- Monitoring of Inhibitors of Enzymatic Amplification in Polymerase Chain Reaction and Evaluation of Efficacy of RNA Extraction for the Detection of Hepatitis C Virus Using the Internal Control
- Quantification of Relative Expression of Genes with Homologous Sequences Using Fluorescence-Based Single-Strand Conformation Polymorphism Analysis – Application to Lactate Dehydrogenase and Cyclooxygenase Isozymes
- Automation of Polymerase Chain Reaction-Based Systems for Detection of Hepatitis C Virus RNA
- A Simple PCR with Different 3′ Ends of the Third Primer for Detection of Defined Point Mutations: HCV Genotyping as an Example
- Two Quantitative Methods for Imaging Samples in Molecular Biology
- Restriction Digest PCR (RD-PCR) for the Analysis of Gene Mutations. Application to Ki-ras
- Direct Detection of Mycobacterium tuberculosis in Respiratory Specimens Using an Automated DNA Amplification Assay and a Single Tube Nested Polymerase Chain Reaction (PCR)
- Quantitative Detection of Hepatitis B Virus DNA with a New PCR Assay
- Molecular Diagnostics for Cardiovascular Disease
- Association of Apolipoprotein E Polymorphism and Concentration with Serum Lipids and Apo-lipoprotein Level in the Chinese fromShanghai
- Angiotensin Converting Enzyme Gene Polymorphism in Coronary Artery Disease: The Indian Scenario
- Mutation C677T of Methylenetetrahydrofolate Reductase Gene Is Not Associated with Coronary Artery Disease, but Possibly with Albuminuria, in Type 2 Diabetic Patients
- Cholesteryl Ester Transfer Protein Mutations, Protein Activity and HDL-Cholesterol Concentration
- Molecular Biology of Alcohol Dependence, a Complex Polygenic Disorder
- Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR
- The Impact of Molecular Diagnosis on Familial Colorectal Cancer
- Screening for Tumour Suppressor p16(CDKN2A) Germline Mutations in Israeli Melanoma Families
- CGG Repeat Interruptions in the FMR1 Gene in Patients with Infantile Autism
- Detection of CYP2D6*3 and 2D6*4 Allelic Variants by PCR-Restriction Fragment Length Polymorphism
- Genetic Basis of Congenital Hypothyroidism: Abnormalities in the TSHβ Gene, the PIT1 Gene, and the NIS Gene
- Follitropin (FSH) Deficiency in an Infertile Male due to FSHβ Gene Mutation. A Syndrome of Normal Puberty and Virilization but Under-developed Testicles with Azoospermia, Low FSH but High Lutropin and Normal Serum Testosterone Concentrations
- Molecular Analysis and Electromyoneurographic Abnormalities in Croatian Children with Proximal Spinal Muscular Atrophies
