Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates
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Chinnarjan Ravindran
Abstract
Alkaline cellulases were screened from 54 fungal isolates obtained from mangrove leaves and mangrove wood litters as endophytes and wood litter fungi, respectively. Only three fungal isolates tested positive for alkaline cellulases under plate assay, among which Chaetomium sp. (NIOCC 36) was found to grow in a wide range of pH (between 4 and 12). This alkaline tolerant fungus was further tested for production of alkaline cellulases (β-endoglucanase, β-exoglucanase, β-glucosidase) using agricultural and industrial wastes as substrates in submerged fermentation (SMF) and solid state fermentation (SSF) processes. Cotton seed under SSF conditions had maximum enzyme production at high alkaline pH. Cellulase enzymes produced under alkaline cotton seed SSF conditions at 50°C had higher stability and activity than under non-alkaline conditions (range tested: pH 5, 7, 12). The agricultural and industrial wastes used as carbon sources were economical for commercial production of cellulose. The high specific activity, and stability of cellulases under neutral to alkaline pH and high temperature will be of use in various industrial and biotechnological applications.
©2010 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Research articles
- A tropical assemblage of benthic macroalgae on rocky reefs in a temperate zone on the western Baja California peninsula, Mexico
- Dynamics of seagrass and macroalgal assemblages in Saipan Lagoon, Western Pacific Ocean: disturbances, pollution, and seasonal cycles
- Salinity tolerance of Rhizophora mucronata Lam. from Gujarat coasts of India
- Life history and morphological studies of Punctaria tenuissima (Chordariaceae, Phaeophyceae), a new record for the Azores
- Symphyocladia lithophila sp. nov. (Rhodomelaceae, Ceramiales), a new Korean red algal species based on morphology and rbcL sequences
- First occurrence of the genus Ostreopsis (Dinophyceae) in the Sea of Japan
- Antioxidant activity of seagrasses and seaweeds
- Anti-inflammatory, antioxidant and antimicrobial activities of aqueous and organic extracts from Dictyopteris membranacea
- A potent antitumor polysaccharide from the edible brown seaweed Hydroclathrus clathratus
- Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates
- Marine fungi from two sandy Mediterranean beaches on the Egyptian north coast
