FITC-Phalloidin Staining of F-Actin in Aglaothamnion oosumiense and Griffithsia japonica (Rhodophyta)
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G. H. Kim
Abstract
Fluorescein isothiocyanate (FITC) conjugated phalloidin was used to label F-actin in Aglaothamnion oosumiense Itono, and Griffithsia japonica Okamura. Labelling was achieved without treatment by wall-digesting enzymes. Formaldehyde fixation followed by Triton X-100 wash gave good preservation of F-actin and cell components. Extensive arrays of F-actin were observed in the peripheral cytoplasm and nuclear area of the two species. Actin was strongly associated with the pit plugs in both species. Quantitative analysis using confocal microscopy showed that the amount of F-actin increased during cell division in Aglaothamnion oosumiense. Actin also appeared to play an important role in cytokinesis and karyokinesis during tetrasporogenesis.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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- Checklist of Mediterranean Seaweeds. III. Rhodophyceae Rabenh. 1. Ceramiales Oltm.
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- Distribution and Dominance of Two Introduced Turf-forming Macroalgae on the Coast of Tuscany, Italy, Northwestern Mediterranean Sea in Relation to Different Habitats and Sedimentation
