Phosphoenolypyruvate Carboxykinase from the Marine Diatom Skeletonema costatum and the Phaeophyte Laminaria setchellii. II. Immunological Characterization and Subcellular Localization
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A. Cabello-Pasini
Abstract
Phosphoenolpyruvate carboxykinase (PEPCK) is responsible for light-independent carbon fixation (LICF) processes in a wide range of marine algae, however, little is known about the intracellular localization of the enzyme among different algal groups. Antibodies against PEPCK recognized polypeptides in electrophoresed samples of cell-free extracts from only non-green phytoplankton and macrophytic Phaeophyta while antibodies against phosphoenolpyruvate carboxylase (PEPC) recognized polypeptides in samples of only chlorophytes. This supports the hypothesis that PEPCK is the dominant enzyme in LICF processes in chromophytes while PEPC is the enzyme responsible for non-photosynthetic processes in the chlorophytes. The enzyme PEPCK was immuno-localized in the chloroplasts of the diatom Skeletonema costatum and the kelp Laminaria setchellii which differs from the localization of the enzymes in most vascular plants. While LICF processes occur in the cytoplasm of most vascular plants, the results of this study suggest that in marine chromophytes, LICF processes are catalyzed in the chloroplasts and that the flow of carbon varies between these groups of autotrophs.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Phosphoenolypyruvate Carboxykinase from the Marine Diatom Skeletonema costatum and the Phaeophyte Laminaria setchellii. II. Immunological Characterization and Subcellular Localization
- Dispersal of Caulerpa racemosa Fragments in the Mediterranean: Lack of Detachment Time Effect on Establishment
- Additions to the Marine Algal (Seaweed) Flora of the Azores
- Effect of Nutrient Availability on Macrocystis pyrifera Recruitment and Survival near Its Southern Limit off Baja California
- Reproductive Allocation and Strategy of Sargassum elegans Suhr and Sargassum incisifolium (Turner) C. Agardh from Reunion Rocks, KwaZulu-Natal, South Africa
- Heydrichia homalopasta sp. nov. (Sporolithaceae, Rhodophyta) from Australia
- The Structure of the Polysaccharide from Hymenocladia sanguinea through 13C NMR Spectroscopy
- Evaluation of the Structure of the Polysaccharide from Myriogramme denticulata as Determined by 13C NMR Spectroscopy
- Ultrastructure of Asci and Ascospores of Massarina velatospora from Intertidal Mangrove Wood
- Two Species of Sphacelaria (Sphacelariales, Phaeophyceae), S. solitaria (Pringsheim) Kylin and S. recurva sp. nov. from Korea
- Spore-Expulsion Rates and Extents of Blade Occupation by Ascomycetes of the Smooth-Cordgrass Standing-Decay System
- Lipid and Fatty Acid Composition during Vegetative and Resting Stages of the Marine Diatom Chaetoceros salsugineus
- The Change in Macroalgal Assemblages through the Saldanha Bay/Langebaan Lagoon Ecosystem (South Africa)
