Development of RAPD Markers for Parentage Analysis in Laminaria digitata
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C. Billot
Abstract
Random Amplified Polymorphic DNA (RAPD) markers were developed for genotyping in Laminaria digitata. Reproducible RAPD banding patterns were reliably obtained, using a quick micro-scale DNA extraction procedure. Intraspecific polymorphism was observed, allowing for unambiguous genotyping within sets of 3–4 sporophytes. A controlled crossing indicated that in this kelp RAPD markers are transmitted to the progeny in a Mendelian way. This approach was then applied to parentage analysis within a set of 30 anonymous sporophytes from a random cross experiment involving 4 parents. The parents of 26 offsprings were unambiguously identified, indicating that cross-fertilisation largely, if not totally, prevailed over self-fertilisation.
Copyright (c)1999 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Greetings to Professor Tseng Cheng-Kwei on the Occasion of His 90th Birthday
- On the Gracilaria in the Western Pacific and the Southeastern Asia Region
- Liagora tsengii sp. nov. (Liagoraceae, Nemaliales) from the Lesser Antilles, West Indies
- A Screening for the Antiviral Effect of Extracts from Brazilian Marine Algae against Acyclovir Resistant Herpes Simplex Virus Type 1
- Seasonal Patterns in the Photosynthetic Capacity, Tissue Pigment and Nutrient Content of Different Developmental Stages of Undaria pinnatifida (Phaeophyta: Laminariales) in Port Phillip Bay, South-Eastern Australia
- Intertidal Fungi in Mauritian Mangroves
- Chaetocerus furcillatus Bailey in the Canadian Maritimes
- Intertidal Macroalgal Community Structure in Southwestern Prince William Sound, Alaska
- In Vitro Carpospore Release and Germination in Kappaphycus alvarezii (Doty) Doty from Tawi-Tawi, Philippines
- On the Distribution and Continuous Expansion of the Japanese Seaweed - Sargassum muticum - in Sweden
- Spatial and Seasonal Variations in the Biological Characteristics of Two Invasive Brown Algae, Turbinaria ornata (Turner) J. Agardh and Sargassum mangarevense (Grunow) Setchell (Sargassaceae, Fucales) Spreading on the Reefs of Tahiti (French Polynesia)
- Development of RAPD Markers for Parentage Analysis in Laminaria digitata
