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Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus
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Kajohn Boonrod
Published/Copyright:
August 29, 2011
Abstract
The multifunctional helper component proteinase (HC-Pro) of potyviruses contains an autoproteolytic function that, together with the protein 1 (P1) and NIa proteinase, processes the polyprotein into mature proteins. In this study, we analysed the autoproteolytic active domain of zucchini yellow mosaic virus (ZYMV) HC-Pro. Several Escherichia coli-expressed MBP:HC-Pro:GFP mutants containing deletions or point mutations at either the N- or C-terminus of the HC-Pro protein were examined. Our results showed that amino acids essential for the proteolytic activity of ZYMV HC-Pro are distinct from those of the tobacco etch virus HC-Pro, although the amino acid sequences in the proteolytic active domain are conserved among potyviruses.
Received: 2011-7-19
Accepted: 2011-7-20
Published Online: 2011-8-29
Published in Print: 2011-10-1
©2011 by Walter de Gruyter Berlin Boston
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Keywords for this article
autoproteolytic activity;
helper component proteinase;
site-directed mutagenesis
Articles in the same Issue
- REVIEW
- Disruption of virus-host cell interactions and cell signaling pathways as an anti-viral approach against influenza virus infections
- PROTEIN STRUCTURE AND FUNCTION
- Conformational changes of the chaperone SecB upon binding to a model substrate – bovine pancreatic trypsin inhibitor (BPTI)
- Iron-catalyzed oxidation of Trp residues in low-density lipoprotein
- MOLECULAR MEDICINE
- Influenza A virus infection activates cholesterol sulfotransferase (SULT2B1b) in the lung of female C57BL/6 mice
- CELL BIOLOGY AND SIGNALING
- Lack of ADAM15 in mice is associated with increased osteoblast function and bone mass
- Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells
- Ligand-independent activation of the arylhydrocarbon receptor by ETK (Bmx) tyrosine kinase helps MCF10AT1 breast cancer cells to survive in an apoptosis-inducing environment
- Insulin modulates glucose-dependent insulinotropic polypeptide (GIP) secretion from enteroendocrine K cells in rats
- Toxicity of Alzheimer's disease-associated Aβ peptide is ameliorated in a Drosophila model by tight control of zinc and copper availability
- PROTEOLYSIS
- In-cell selectivity profiling of membrane-anchored and replicase-associated hepatitis C virus NS3-4A protease reveals a common, stringent substrate recognition profile
- Analysis of the autoproteolytic activity of the recombinant helper component proteinase from zucchini yellow mosaic virus
